ATF-2 Human
Activating Transcription Factor-2 Human Recombinant
Catalog No. PKA-306
Synonyms:
Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, cAMP response element-binding protein CRE-BP1, HB16, CREB2, TREB7, MGC111558, ATF2.
Introduction:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. The protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. Additional transcript variants have been identified but their biological validity has not been determined.
Description:
Activating Transcription Factor-2Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain encoding 19-96 amino acids and having a molecular mass of 57 kDa.
The ATF-2 is manufactured as a maltose binding protein (MBP) fusion protein with an amino terminal polyhistidine tag.
ATF-2 is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Formulation:
ATF-2 is supplied as lyophilized freeze dry powder without additives.
Unit Definition:
ATF2 is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with immunoprecipitated JNK from stimulated cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to ATF2 [pT71], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Please note: Kinase activity may vary depending on the substrate and reaction conditions.
Storage Conditions:
Store at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Purity:
Greater than 90% as determined by SDS-PAGE.
Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
上海希美化学有限公司
公司地址
上海市松江区陆家圈路168弄113号
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