重组人泛素结合酶E2K(Human UBE2K)

UBE2K Human
Ubiquitin-Conjugating Enzyme E2K Human Recombinant
Catalog No. ENZ-339

Synonyms:
Ubiquitin-conjugating enzyme E2-25 kDa, EC 6.3.2.19, Ubiquitin-protein ligase, Ubiquitin carrier protein, E2(25K), Huntingtin-interacting protein 2, HIP-2, LIG, HYPG, UBE2K, UbcH1.

Introduction:
Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, also known as HIP2, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyse the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM. Recently, HIP2 (or UbcH1) has been shown to bind to the N terminus of Huntington and may play a role in Huntington disease.

Description:
Ubiquitin-Conjugating Enzyme E2K Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 208 amino acids and having a molecular mass of 23.4 kDa.
The UBE2K protein contains 6xHis tag and is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
Lyophilized from a 0.2μm filtered concentrated (1 mg/ml) solution in
1X PBS and 1mM DTT, pH 7.5.

Solubility:
It is recommended to reconstitute the lyophilized HIP-2 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized HIP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2K should be stored at 4°C between 2-7 days and for future use below
-18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
MHHHHHHAMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRG
EIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLD
ILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFK
QTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDAVIVALSSKSWDVETA
TELLLSN.

Usage:
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY.They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

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