重组人白介素-2(Human IL-2)

Prospec (蛋白质专家) 是一家领先的生物科技公司，有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子，生长因子，趋化因子，激素，酶，病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利，所以Prospec以生产成本低，生产效率高，产品纯度高，有竞争力的价格，使Prospec产品在市场上立于不败之地（占有很大优势）。
Prospec为满足顾客的需要，还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构，政府研究部门，生物科技和药物公司以及医院和参照实

IL 2 Human
Interleukin-2 Human Recombinant
Catalog No. CYT-209 
Synonyms:
T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Introduction:
IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

Description:
Interleukin-2 Human Recombinant produced in E.Coli is a single, non-glycosylated mutein (variant form) of human IL-2 polypeptide chain containing 134 amino acids and having a molecular mass of 15517 Dalton.
Our Interleukin -2 has an Ser substitute for Cysteine at position 126.
The IL-2 is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein (1.1mg/ml) was lyophilized after extensive dialysis against 0.17mg sodium monobasic & 0.89mg dibasic sodium phosphate buffer to a pH=7.5.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser.

Biological Activity:
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is < 0.0645 ng/ml, corresponding to a Specific Activity of 16.9 MIU/mg.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.614 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
Related Products

» IL 2 Antibody
» IL 2 Antibody
» IL 2 Human, His
» IL 2 Human, Yeast
» IL 2 Mouse
» IL 2 Mouse, His
» IL 2 Porcine
» IL 2 Rat
» IL 2r Antibody
» IL 2r Antibody
» sIL 2R Human
» sIL 2R Human, GST