重组人肝细胞生长因子CHO(Human HGF CHO)

Prospec (蛋白质专家) 是一家领先的生物科技公司，有16年生产细菌重组蛋白方面的专业经验。Prospec还生产细胞因子，生长因子，趋化因子，激素，酶，病毒抗原及其它的重组蛋白。
Prospec的蛋白质提纯技术申请了专利，所以Prospec以生产成本低，生产效率高，产品纯度高，有竞争力的价格，使Prospec产品在市场上立于不败之地（占有很大优势）。
Prospec为满足顾客的需要，还根据顾客需求进行重组蛋白生产服务。
Prospec为全球的学术机构，政府研究部门，生物科技和药物公司以及医院和参照实

HGF Human CHO
Hepatocyte Growth Factor Human Recombinant CHO
Catalog No. CYT-251 
Synonyms:
Scatter Factor (SF), Hepatopoietin (HPTA), HGF.

Introduction:
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3and GM-CSFto stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.


Description:
Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an α-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa.
The HGF is purified by proprietary chromatographic techniques.


Source:
Chinese Hamster Ovary Cells


Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.


Solubility:
It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.





Purity:
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Amino acid sequence:
Agrees with the sequence of native human HGF.

Biological Activity:
The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard.


Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. 
Related Products

» HGF A Human
» HGF B Human
» HGF Human
» pHGF Porcine