Catalogue Number |
PRO-032 |
Synonyms |
ADP-ribosylation factor 6 |
Introduction |
ARF6 is a part of the ADP ribosylation factor family of GTP-binding proteins. ARF6 is restricted to the plasma membrane, and controls vesicular trafficking, remodeling of membrane lipids, and signaling pathways which lead to actin remodeling. Furthermore, ARF6 is a key player in conservation of organelle integrity, assembly of coat proteins and activation of phospholipase D. |
Description |
ARF6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 195 amino acids (1-175a.a.) and having a molecular mass of 22.2kDa.
ARF6 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile Filtered clear solution. |
Formulation |
The ARF6 protein solution (1mg/1ml) is formulated in In 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 0.2mM PMSF and 20% glycerol. |
Amino Acid Sequence |
MGSSHHHHHH SSGLVPRGSH MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS |
Purity |
Greater than 95% as determined by SDS-PAGE. |
Storage |
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
Please avoid freeze thaw cycles. |
Usage |
ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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