Catalogue Number |
PRO-867 |
Synonyms |
ARF-1, ADP-ribosylation factor 1. |
Introduction |
ARF1 is a small guanine nucleotide-binding protein that increases the enzymatic activities of cholera toxin. ARF1 is necessary and ubiquitous in eukaryotes. ARF1 takes part in vesicular transport and functioning via phospholipase D activation. ARF1 is involved in membrane traffic and organelle integrity which are closely tied to their reversible association with membranes and distinct interactions with membrane phospholipids. |
Description |
ARF1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The ARF1 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile filtered colorless solution. |
Formulation |
ARF1 Human solution containing 20mM Tris pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol. |
Stability |
ARF1 Human although stable at 4°C for 1 week, should be stored desiccated
below -18°C.
Please prevent freeze thaw cycles. |
Purity |
Greater than 90.0% as determined by SDS-PAGE. |
Amino acid sequence |
MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K. |
Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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