AMPK gamma-1(C-term)(PRKAG1)antibody
Cat.#: 1592-1
Rabbit Monoclonal Antibody
Clone ID: Y308
Swiss Prot: P54619
Mol Weight: 38kDa
Size: 100ul
Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (2). AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro (3).
Recommended Applications
WB, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000
IP: 1:50
FC: 1:20
Species Reactivity
Human, Mouse, Rat
Cross reactivity determined by western blot only.
Products Data
A. Western blot analysis on Jurkat cell lysate using anti-AMPK gamma-1 (C-term) RabMAb (cat. # 1592-1), dilution 1:1,000.
Specificity
A synthetic peptide corresponding to residues in the C-term of human AMPK gamma-1 subunit was used as an immunogen
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
PRKAG1 antibody, AMPKG antibody, MGC8666 antibody, 5'-AMP-activated protein kinase subunit gamma-1 antibody, antibody
Description References
1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136
3. Carling, D., Zammit, V. A,, and Hardie, D. G. (1987) FEES Lett. 223,217-222
Product References
1. Anderson KA, Ribar TJ, Lin F, et al, Hypothalamic CaMKK2 Contributes to the Regulation of Energy Balance ,Cell Metab 7 (2008) 377-388
[Application: WB]
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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