AMPK beta 1(PRKAB1)antibody
Cat.#: 1604-1
Rabbit Monoclonal Antibody
Clone ID: Y367
Swiss Prot: Q9Y478
Mol Weight: 38kDa
Size: 100ul
Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1). AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).
Recommended Applications
WB, IHC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100-250
IP: 1:80
FC: 1:50
Species Reactivity
Human, Mouse, Rat
Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
Specificity
A synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
PRKAB1 antibody, AMPK antibody, HAMPKb antibody, MGC17785 antibody, 5'-AMP-activated protein kinase subunit beta-1 antibody, antibody
Description References
1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364
2. Woods, A.; Cheung, P. C. F.; Smith, F. C.; Davison, M. D.; Scott, J.; Beri, R. K.; Carling, D.: J. Biol. Chem. 271: 10282-10290, 1996.
3. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136
4. Thornton, C.; Snowden, M. A.; Carling, D.: J. Biol. Chem. 273: 12443-12450, 1998.
Product References
1. Bendayan M, Londono I, Kemp BE, et al, Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immuno-electron Microscopy ,J. Histochem. Cytochem. 57 (2009) 963 - 971
[Application: WB]
2. Anderson KA, Ribar TJ, Lin F, et al, Hypothalamic CaMKK2 Contributes to the Regulation of Energy Balance ,Cell Metab 7 (2008) 377-388
[Application: WB]
3. Lessard SJ, Rivas DA, Chen ZP, et al, Impaired Skeletal Muscle β-Adrenergic Activation and Lipolysis Are Associated with Whole-Body Insulin Resistance in Rats Bred for Low Intrinsic Exercise Capacity ,Endocrinology 150 (2009) 4883 - 4891
[Application: WB]
4. Dzamko N, van Denderen BJ, Hevener AL, et al, AMPK 1 Deletion Reduces Appetite, Preventing Obesity and Hepatic Insulin Resistance ,J Biol Chem 285 (2010) 115-122
[Application: WB]
5. Bendayan M, Londono I, Kemp BE, et al, Association of AMP-activated Protein Kinase Subunits With Glycogen Particles as Revealed In Situ by Immunoelectron Microscopy ,J. Histochem. Cytochem. 57 (2009) 963 - 971
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企业名称
上海希美化学有限公司
企业信息已认证
企业类型
信用代码
310228001203103
成立日期
2009-05-31
注册资本
10
经营范围
化工原料及产品(除危险化学品、监控化学品、烟花爆竹、民用爆炸物品、易制毒化学品),实验室设备,仪器仪表,金属制品,电子产品,机电产品销售,从事化工科技、计算机科技领域内技术开发、技术咨询、技术服务。【依法须经批准的项目,经相关部门批准后方可开展经营活动】
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