Alpha-Tubulin(TUBA1B)antibody

Alpha-Tubulin(TUBA1B)antibody
Cat.#: 1878-1
Rabbit Monoclonal Antibody
Clone ID: EP1332Y
Swiss Prot: P68363
Mol Weight: 50kDa
Size: 100ul

Description
Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis. It consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). For both type of tubulin, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at microtubule surface (3). A mutation on alpha tubulin has been linked to abnormal neuronal migration and neurodevelopmental disorders in humans (4).

Recommended Applications
WB, IHC, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IHC: 1:250 - 500
ICC: 1:250 - 500
IP: 1:10 - 100
FC: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

Specificity
A synthetic peptide corresponding to residues near the N-term of human α-Tubulin was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
TUBA1B antibody, K-ALPHA-1 antibody, Tubulin alpha-1B chain antibody, Alpha-tubulin ubiquitous antibody, Tubulin K-alpha-1 antibody, Tubulin alpha-ubiquitous chain antibody

Description References
1. Wang HW et al. Nature 435: 911-915, 2005.
2. Cleveland DW et al. Cell 20: 95-105, 1980.
3. Westermann S, Weber K, Nat. Rev. Mol. Cell Biol. 4:938 -947, 2003.
4. Keays D, et al. Cell 128:45-47, 2007.

Product References
1. Gendron TF, McCartney S, Causevic E, et al, Ethanol enhances tau accumulation in neuroblastoma cells that inducibly express tau ,Neuroscience Letters 443 (2008) 67-71
[Application: WB]
2. Wang XL, Su B, Siedlaka SL, et al, Amyloid-β overproduction causes abnormal mitochondrial dynamics via differential modulation of mitochondrial fission/fusion proteins ,PNAS 105 (2008) 19318 - 19323
[Application: WB, ICC]
3. Wang XL, Su B, Lee HG, et al, Impaired Balance of Mitochondrial Fission and Fusion in Alzheimer's Disease ,J. Neurosci. 29 (2009) 9090 - 9103
4. Ko LW, Ko HHC, Lin WL, et al, Aggregates Assembled From Overexpression of Wild-Type α-Synuclein are not Toxic to Human Neuronal Cells ,J Neuropathol Exp Neurol 67 (2008) 1084-1096
[Application: WB]