Recombinant Human BMP-2 CHO(重组人骨形态发生蛋白-2 CHO)

BMP 2 Human, CHO
Bone Morphogenetic Protein-2 Human Recombinant, CHO
Catalog No. CYT-650 
Synonyms:
BMP-2, BMP2A, Bone morphogenetic protein 2, BMP2, BMP-2A.


Introduction:
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.


Description:
Bone Morphogenetic Protein-2 Human Recombinant produced in CHO is a homodimeric, glycosylated, Polypeptide chain containing 115 amino acids and having a calculated molecular mass of 26018 Dalton. Due to its glycosilation, BMP-2 migrates on SDS-PAGE under non-reducing conditions at 36kDa and 18kDa under reducing conditions.
The BMP-2 CHO derived protein is purified by proprietary chromatographic techniques.


Source:
Chinese Hamster Ovary Cell




Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation:
1mcg of BMP-2 was lyophilized from a sterile solution containing 0.2% sucrose, 2.5% glycine, 0.01% tween-80, 0.01% NaCl and 3% mannitol.

Solubility:
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile water at a concentration of 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity:
The ED50 as determined by cytolysis of ATDC-5 cell is less than 50ng/ml.

Usage:
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.