【优品分享】Quantifoil-UltrAuFoil®

UltrAuFoil® R 1.2/1.3

For high-resolution

transmission electron

microscopy

So you benefit from

So you benefit from Up to 0.5 Å increase in resolution on replacing traditional holey carbon supports with UltrAuFoil®.¹

The highest resolution data from your initial movie frames before radiation damage accumulates, due to reduced beam-induced motion.² ³

Reduced image distortion because of lowered static and semi-mobile charge accumulation as gold is highly conductive at liquid nitrogen temperatures.²

Samples protected from damage due to accumulating positive charge by neutralization with secondary electrons generated by irradiating adjacent gold.²

Biomolecules retaining native structure and not being put under mechanical strain from foil crinkling.

Improved particle distribution as the gold foil

is even flatter than carbon.²

Easier grid surveying due to the high contrast of the gold foil.²

References

Danev, R. et al. Routine sub-2.5 Å cryo-EM structure determination of GPCRs. Nat. Commun. 12: 4333 (2021).

Russo, C. R. & Passmore, L. A. Ultrastable gold substrates: Properties of a support for high-resolution electron cryomicroscopy of biological specimens. J. Struct. Biol. 193: 33-44 (2016).

Russo, C. R. & Passmore, L. A. Ultrastable gold substrates for electron cryomicroscopy. Science 346: 1377-1380 (2014).

Amil, S. M. et al & Fernandez-Leiro, R. The cryo-EM Structure of Thermotoga maritima β-Galactosidase: Quaternary Structure Guides Protein Engineering. ACS Chem. Biol. 15: 179-188 (2020).

The optimum grid and support geometry depends on many variables, but the following general guidelines will help you select the perfect UltrAuFoil® Holey Gold support.

Larger particle sizes require a larger hole size to ensure sufficient numbers of particles in an image:

Three combinations of grid mesh size and foil geometry have been chosen to maximize compatibility with high-resolution cryo-EM data collection.