摘 要 为适应生命科学对高精度分离分析方法的要求，发展高选择性、高灵敏度的检测技术用于毛细管电泳 (CE) 一直是分析科学的研究热点。近年来，我们实验室对于CE 三联吡啶钌 [Ru(bpy)32+] 电化学发光 (ECL)，即CE-ECL 方法进行了系统研究，研制成ECL仪、CE-ECL仪，发展了ECL试剂固定化检测及ECL试剂衍化检测等技术，并应用于临床药物分析。本文对CE-ECL的检测原理、仪器装置、新型检测池、CE-ECL最新发展、在药物和临床分析等方面的应用予以介绍，并对CE-ECL在生命科学中的基因与蛋白质分析、重大疾病标识物的筛选与检测、临床诊断的应用及其微全分析等领域进行展望。 关键词 ：毛细管电泳 电化学发光 生化分析

This article presents the state of the art of analytical applications of the electrochemiluminescence (ECL) of tris (2,20-bipyridyl) ruthenium(Ru(bpy) 2þ 3 ) and its derivatives. In the last seven years, Ru(bpy) 2þ 3 ECL has attracted much interest from analysts and been successfully exploited as a detector of flow injection analysis (FIA), high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and micro total analysis systems (TAS). Immobilization of Ru(bpy) 2þ 3 on a solid surface provides several advantages over the solution-phase ECL procedure, such as the simplicity of experimental design and cost-effectiveness. After a brief discussion of the mechanism of Ru(bpy) 2þ 3 ECL, we discuss its applications in FIA, HPLC, CE and TAS and give special attention to the design of Ru(bpy) 2þ 3 ECL cells and some immobilization techniques of Ru(bpy) 2þ 3 ; we focus on papers published after 1997.