菌落总数测定是食品微生物检验中应用得最为广泛一个检测方法，其国标方法文本屡经修订，不断完善。历经了1984版、1994版、2003版、2008版、2010版、2016版到今天的2022版，在38年间已经陆续修订发布了7个版本，平均5年修订一次，这应该是GB4789系列里之最了，恐怕在国家标准中也不多见，足见其重要性。

Energy digestibility in dog and cat food can be predicted by the linear regression of the fiber content on a dry matter (DM) basis. Fiber is the independent variable and in vivo measured energy digestibility the dependent variable. This has been demonstrated for crude fiber (CF) in both species and in dogs for total fiber as analyzed by the Englyst and Cummings method (1,2). To date, however, few observations have been published measuring both total dietary fiber (TDF, according to the method of Prosky et al. [3]) and energy digestibility in dogs and cats. From the existing data points, it is thus not possible to 1) calculate a regression equation as for crude fiber or total fiber that is likely to predict digestibility more accurately in pet foods and 2) compare the accuracy of prediction of energy digestibility by CF and TDF methods.