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RF-O2荧光光纤氧气测量技术——氧气测量全面解决方案
RF-O2荧光光纤氧气测量技术是基于REDFLASH光极传感器技术的最先进的氧气测量技术,由欧洲Pyroscience公司及Graz大学等科学家研制生产,由光极氧气传感器、测量仪及软件组成,广泛应用于环境科学、生态科学、植物科学、动物科学、海洋科学、生物医学、生物技术、食品科学等各个领域,其主要功能特点如下
1) REDFLASH光极氧气传感器技术,高精确度、高稳定性、高时空解析度、低能耗、无耗氧、无交叉敏感性
2) 传感器类型灵活多样,有探头式、探针式、非接触式(sensor spot)及纳米微粒式等,适应于液体和气体不同条件下的O2测量
3) 有内置sensor spot的流通管和呼吸瓶,非接触式测量流动液体的溶解氧及呼吸瓶内液体或气体中氧气含量
4) 轻便紧凑型FireStingO2测量仪,内置水汽、气压传感器,有1、2、4通道供选配,可分别接1个、2个、4个光极氧气传感器,另有Mini型FireStingO2-mini供选配
5) U盘式PiccolO2测量仪——世界上最小的O2测量仪,可连接一个O2传感器,USB口连接电脑,即插即用
测量原理:
REDFLASH光极O2传感器技术,利用独特的O2敏感REDFLASH指示剂,通过610-630nm调制红光激发,REDFLASH指示剂发出760-790nm红外荧光,荧光强度随接触的O2分子浓度升高而发生荧光淬灭,这种荧光动态通过光纤传输到测量仪,测量仪灵敏地检测其相位漂移并据此换算成O2浓度
应用领域:
1) 水体溶解氧测量监测、藻类及藻类生物膜光合作用与呼吸作用测量监测
2) 植物光合作用与呼吸作用测量监测
3) 水生动物(鱼类、水生昆虫等无脊椎动物、浮游动物等呼吸代谢测量
4) 陆生动物、实验动物、动物组织、血液等呼吸代谢测量
5) 土壤、湿地、海洋沉积、河湖沉积剖面O2测量
6) 生物反应器、发酵过程、酶动力学、细胞培养等O2测量监测
7) 粮食食品储运、葡萄酒等O2测量监测
8) 污水处理、沼气、垃圾填埋场、有机物降解等O2测量监测
技术指标:
1) FireStingO2(FSO2)测量仪:
a) 有1通道、2通道、4通道可供选配,分别可接1个、2个和4个O2传感器,可并联组成8通道甚至更多通道;另具备一个温度传感器通道(可选配4通道温度传感器)
b) 激发光源620nm,监测器760nm(NIR)
c) 采样频率:每秒4次
d) 内置气压传感器,300-1100mbar,0.06mbar分辨率,精确度±3mbar
e) 内置湿度传感器,0-100%,分辨率0.04%,精确度±0.2%
f) 内置温度传感器,-40-125°C,分辨率0.01°C,精确度±0.3°C
g) 具模拟输出和自动模式,0-2.5VDC
h) USB接口,通过USB口PC供电
i) 大小:68x120x30mm,重350g
2) PiccolO2 U盘式测量仪:大小仅15x15x54mm,重量约20g,单通道,激发光620nm,检测器760nm,采样频率每秒20次。可并联组成多通道测量系统。可通过PiccoTHP测量温湿度和气压并进行补偿
3) 探头式O2传感器:直径3mm,测量范围0-50%(0-23mg/l)(可选配其它范围),检测极限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2,精确度±0.2%(0.1mg/l)@20% O2,最低使用寿命1千万数据点,存储时间大于3年(室温暗处储放)
4) 探针式O2传感器:有固定探针式、可伸缩探针式、尖头式及圆头式等不同类型供选配;探针直径有50μm、230μm、430μm等规格;测量范围0-50%(0-23mg/l)(可选配其它范围),检测极限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2,精确度±0.2%(0.1mg/l)@20% O2,最快响应时间小于1s(与探针粗细有关),最低使用寿命1百万数据点,存储时间大于3年(室温暗处储放)
5) 非接触式(sensor spot)O2传感器(见下左图):用于非接触性测量监测透明容器中的氧气含量,传感器贴用硅胶等贴附在容器内壁,通过固定在外壁的光纤将荧光动态信号传输到测量仪以检测O2浓度;测量范围0-50%(0-23mg/l)(可选配其它范围),检测极限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2,精确度±0.2%(0.1mg/l)@20% O2,最低使用寿命2千万数据点,存储时间大于3年(室温暗处储放)
6) 纳米微粒传感器(参见上右图):纳米技术,用于非接触性测量微量液体中O2含量,即时响应,测量范围0-50%(0-23mg/l),检测极限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2,存储时间大于3年(室温暗处储放)
7) 流通管:内置非接触式O2传感器,用于流动液体O2测量监测(如鱼类呼吸代谢测量等),测量范围0-50%(0-23mg/l)(可选配其它范围),检测极限0.02%(0.01mg/l),分辨率0.05%(0.025mg/l)@20% O2,精确度±0.2%(0.1mg/l)@20% O2,最低使用寿命1千万数据点,存储时间大于3年(室温暗处储放)
8) 呼吸瓶:内置非接触式O2传感器,用于生物呼吸测量(如藻类、小型鱼类、鱼卵、昆虫等),标准配置有4ml和20ml两种规格
9) Pyro Oxygen Logger软件用于参数设置、校准、数据显示包括图表显示、数据输出等功能
应用案例:
案例1:法国Bordeaux大学利用FSO2 4通道荧光光纤氧气测量仪,对Aquitaine海岸沉积样芯耗氧进行了测量分析,以研究海洋底栖动物活动(bioirrigation)对海岸带生态系统生态过程及生物地理化学功能(如沉积有机物的再矿化)的影响。
案例2:芬兰Turku大学利用FSO2和430μm光极氧探针,对南瓜类囊体悬浮液光合放氧进行了测量分析。
案例3:美国Woods Hole海洋学研究所,利用RF-O2非接触式光极氧气传感器(sensor spot),对海洋无脊椎动物呼吸代谢进行了测量分析,以研究其固有的生物钟与环境胁迫的关系,这些海洋无脊椎动物体重只有0.5-50mg。图中为翼足类软体动物在不同浓度CO2条件下的耗氧率。
案例4:澳大利亚海洋科学研究所、瑞典Gothenburg大学等组成的科学小组,利用Pyroscience的REDFLASH氧气测量技术,对河鲈(Perca fluviatilis)呼吸代谢进行测量分析,以研究其热耐受性和适应性的生理机制。他们选择波罗的海核电站附近的一个泻湖,核电站排出的热水进入该泻湖,在过去30年大量鱼类因为不适应水温升高而灭绝,但河鲈却得以繁盛,该地成为理想的研究气候变暖对鱼类种群影响的“天然实验室”。他们测量河鲈呼吸代谢率的同时,还测量其静脉血液在温度升高状态下的氧分压,静脉血是河鲈心脏供氧的主要来源,高温条件下静脉血氧气含量被认为是其心脏功能的重要限制因子。
案例5:德国Ulm大学利用FSO2测量仪和50μm可伸缩式RFO2探针,对患者脑脊髓液(CSF)样品溶解氧进行测量分析,以研究探讨神经紊乱及神经炎等疾病的生理和诊断。
案例6:德国农业科学与景观研究机构,利用FSO2测量仪和RFO2探针,对土壤氧气进行测量,以评估不同种类蚯蚓在低氧条件下对土壤改良的效率。
案例7:西班牙Valladolid大学利用RFO2荧光光纤氧气测量技术,监测葡萄酒橡木桶O2吸收——对葡萄酒品质至关重要但一直以来缺乏科学的了解。葡萄酒在橡木桶内(3-24个月)的过程溶解氧至关重要,因为O2调节了葡萄酒整个的熟化过程。
近期部分参考文献:
2015
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2014
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氧氮分析仪RF-O2的工作原理介绍
氧氮分析仪RF-O2的使用方法?
RF-O2多少钱一台?
氧氮分析仪RF-O2可以检测什么?
氧氮分析仪RF-O2使用的注意事项?
RF-O2的说明书有吗?
氧氮分析仪RF-O2的操作规程有吗?
氧氮分析仪RF-O2报价含票含运吗?
RF-O2有现货吗?
最多添加5台