使用 Thermo Scientific™ Acclaim™ 300 C18 液相色谱柱，可进行高分离度肽图分析和蛋白质分离。 3μm 超纯硅胶颗粒可降低分析物进入固定相的扩散限值，从而可快速进行分离度分离。 与 5μm 色谱柱填料相比，较小的颗粒支持在更短的色谱柱中增加流速并采用更浅的梯度，从而加快分离速度。
高分辨率分离蛋白质和肽
300? 微孔硅胶上的高性能 C18 化学键合
经过应用测试，适用于肽图分析
重现性好，结果可靠
流失率极低，与质谱完全兼容
在 pH 值 2.5 - 7.5 之间保持稳定
关于 Acclaim HPLC 色谱柱系列
选择性互补、柱效高、分离度超高
固定相多种多样，包括常规的 C8 和 C18 固定相、极性嵌入键合相和专用键合相（包括大孔径）
经过性能测试，可确保高质量和良好的可靠性
| Acclaim PepMap for TFA-Free Peptide Mapping Specifications | |||
|---|---|---|---|
| Column type | Nano | Capillary | Micro |
| Column i.d. | 75 μm | 300 μm | 1.0 mm |
| Length | 50, 150, 250, and 500 mm | 50, 150, and 250 mm | 50, 150, and 250 mm |
| Recommended Flow Rate | 200 nL/min | 4 μL/min | 40 μL/min |
| Packing Material | C18, 100 ?, 3 μm | C18, 100 ?, 3 μm | C18, 100 ?, 3 μm |
| C18, 300 ?, 5 μm | C18, 300 ?, 5 μm | C18, 300 ?, 5 μm | |
| C8, 100 ?, 3 μm | C8, 100 ?, 3 μm | C8, 100 ?, 3 μm | |
| C8, 100 ?, 5 μm | C8, 100 ?, 5 μm | C8, 100 ?, 5 μm | |
| C4, 300 ?, 5 μm | C4, 300 ?, 5 μm | C4, 300 ?, 5 μm | |
| Column | Fused-silica | Glass Lined Tubing | Stainless steel |
| Column Inlet | 1/16-in. PEEK sleeve adapter (provided) nanoViper (optional) | 1/16-in. universal fitting nanoViper (optional) 50μm i.d. PEEK sheathed fused silica with nanoViper fittings | 1/16-in. PEEK tubing nanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittings |
| Column Outlet | Fused-silica 25-μm i.d./ 280-μm o.d. | Fused-silica 75-μm i.d./ 280-μm o.d | 1/16-in. PEEK tubing nanoViper (optional) 75μm i.d. PEEK sheathed fused silica with nanoViper fittings |
| MS Outlet | 1-m Length | ||
Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
| Analytical Columns | |
|---|---|
| Acclaim 300, C18, 3 μm Analytical (2.1 x 50 mm) | 060263 |
| Acclaim 300, C18, 3 μm Analytical (2.1 x 150 mm) | 060264 |
| Acclaim 300, C18, 3 μm (3.0 x 150 mm) | 063684 |
| Acclaim 300, C18, 3 μm Analytical (4.6 x 50 mm) | 060265 |
| Acclaim 300, C18, 3 μm Analytical (4.6 x 150 mm) | 060266 |
| Guard Columns | |
|---|---|
| Acclaim 300,C18, 3μm, Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 | 069690 |
| PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA | 069697 |
| Hardware | |
|---|---|
| Acclaim SST Guard Cartridge Holder V-1 | 059456 |
| Guard to Analytical Column Coupler | 059457 |
| Guard Kit (Holder and Coupler) | 059526 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
| Micro and Nano Columns | |
|---|---|
| Acclaim PepMap100, C18, 3 μm, 100 ?, 75 μm i.d. × 5 cm, nanoViper | 164567 |
| 75 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper | 164708 |
| 300 μm i.d. x 5 cm, packed with Acclaim PepMap100 C18, 3 μm, 100?, nanoViper | 164716 |
| 300 μm i.d. x 15 cm, packed with Acclaim PepMap300 C18, 5 μm, 300?, nanoViper | 164718 |
| Acclaim PepMap300, C18, 5 μm, 300 ? Wide Pore, 1.0 mm x 5 cm | 163584 |
| Acclaim PepMap100, C18, 3 μm, 100 ?, 1 mm i.d. × 15 cm, nanoViper | 164572 |
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