Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.
Based on 5 μm ultrahigh purity spherical silica gel particles, with 300 ? pores
Proteins separated under non-denaturing conditions
High protein loading capacity for protein purification applications
Proprietary multidentate silica bonding for enhanced hydrolytic stability
High-resolution HPLC separation of proteins and peptides
Wide range of applications
The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.
| ProPac HIC Columns Specifications | |
|---|---|
| Protein Loading Capacity | 340 mg Lysozyme per 7.8 x 75 mm column |
| Starting Material | Ultrapure Spherical Silica |
| Particle Size | 5 μm |
| Pressure Limit | 4,000 psi |
| Column Construction | Stainless Steel |
| Phase | HIC |
| Average Pore Diameter | 300 ? |
| Endcapped | No |
| Mobile Phase Compatibility | 2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup |
| pH range | pH 2.5 – 7.5 |
| Temperature Limit | < 40 °C |
| Analytical Columns | |
|---|---|
| ProPac HIC-10 Column (2.1 x 100 mm) | 063653 |
| ProPac HIC-10 Column (4.6 x 100 mm) | 063655 |
| ProPac HIC-10 (4.6 x 250mm) | 074197 |
| ProPac HIC-10 Column (7.8 x 75 mm) | 063665 |
ProPac HIC蛋白质和肽柱063665信息由上海希言科学仪器有限公司为您提供，如您想了解更多关于ProPac HIC蛋白质和肽柱063665报价、型号、参数等信息，欢迎来电或留言咨询。
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