Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.
Based on 5 μm ultrahigh purity spherical silica gel particles, with 300 ? pores
Proteins separated under non-denaturing conditions
High protein loading capacity for protein purification applications
Proprietary multidentate silica bonding for enhanced hydrolytic stability
High-resolution HPLC separation of proteins and peptides
Wide range of applications
The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.
ProPac HIC Columns Specifications | |
---|---|
Protein Loading Capacity | 340 mg Lysozyme per 7.8 x 75 mm column |
Starting Material | Ultrapure Spherical Silica |
Particle Size | 5 μm |
Pressure Limit | 4,000 psi |
Column Construction | Stainless Steel |
Phase | HIC |
Average Pore Diameter | 300 ? |
Endcapped | No |
Mobile Phase Compatibility | 2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup |
pH range | pH 2.5 – 7.5 |
Temperature Limit | < 40 °C |
Analytical Columns | |
---|---|
ProPac HIC-10 Column (2.1 x 100 mm) | 063653 |
ProPac HIC-10 Column (4.6 x 100 mm) | 063655 |
ProPac HIC-10 (4.6 x 250mm) | 074197 |
ProPac HIC-10 Column (7.8 x 75 mm) | 063665 |
企业名称
上海希言科学仪器有限公司
企业信息已认证
企业类型
有限责任公司(自然人投资或控股)
信用代码
91310117301434080L
成立日期
2014-05-26
注册资本
人民币100.0000万元整
经营范围
一般项目:技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广;仪器仪表销售;实验分析仪器销售;光学仪器销售;货物进出口;技术进出口。(除依法须经批准的项目外,凭营业执照依法自主开展经营活动)
上海希言科学仪器有限公司
公司地址
上海市松江区九亭镇涞寅路1025号立同国际商务大厦3067室
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