MAbPac Protein A LC 色谱柱
Thermo Scientific? MAbPac? Protein A LC 色谱柱用于收集细胞培养物，可以实现快速而准确的单克隆抗体 (MAb) 滴度分析。 该色谱柱将低反压和高效的优点相结合，可以实现高通量和高准确性的分析。 独特的规格使其适用于生物相容系统，可以快速而便捷的实现加载、结合、淋洗和收集自动化。 该色谱柱为非多孔、聚合物树脂，具有疏水的二乙烯基苯核及亲水表面，专为亲和分离而优化，具有出色的稳健性和可重现性。
快速而准确的 MAb 滴度分析
完整循环时间（包括平衡）仅 2 分钟
较宽的浓度线性范围 (0.025 - 5 mg/mL)，无残留效应
柱压低，在 2mL/min 流速下柱压低于 250 psi
出色的样品回收率
MAbPac Protein A LC 色谱柱可用于滴度分析或浓缩与纯化 IgG 材料，以供后续实验或二维 (2D) 色谱分析使用。 该色谱柱规格为 HPLC 生物惰性、4×35mm PEEK 柱身。
MAbPac Protein A LC 色谱柱
订货信息：
| MAbPac Protein A, 12 μm, Analytical Column (4 x 35mm) | 082539 |
MAbPac Protein A Column for Monoclonal Antibody Titer Analysis
The Thermo Scientific? MAbPac? Protein A column is a high-performance, affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody (MAb) concentration in the harvest cell culture (HCC). Early in the development of recombinant MAbs, a large of number of HCC samples must be screened for IgG titer. Affinity chromatography employing a Protein A ligand is often used to determine the MAb concentration. The MAbPac Protein A column is a high-pressure liquid chromatography (HPLC) column designed to provide fast, accurate, titer analysis with linearity over a wide concentration range without carryover.
The MAbPac Protein A column is based on a novel nonporous polymeric resin consisting of a hydrophobic divinylbenzene core and a hydrophilic surface. This proprietary resin is designed for fast mass transfer, resulting in efficient separations without carryover. The recombinant Protein A ligands are covalently attached onto the hydrophilic resin surface through their amine groups to provide a very rugged, reproducible column. The column is available in a HPLC bioinert, 4 × 35 mm PEEK column body.
Product Highlights:
? A novel nonporous polymeric resin with a hydrophilic surface
?
Functionalized with the recombinant protein A ligands
? Wide range of MAb
quantitation: 0.025–5 mg/mL
? Fast analysis: 2 min/analysis
? Rugged
?
Low pressure: column pressure is less than 250 psi at 2 mL/min
The MAbPac Protein A column can be used for both titer analysis or concentrating and purifying IgG material for later experiments or two-dimensional (2D) chromatography.
| MAbPac Protein A Column Specifications | |
|---|---|
| Column Dimension | 4 × 35 mm |
| Base Matrix Material | Hydrophilic nonporous resin |
| Surface Ligand | Protein A |
| Particle Size | 12 μm |
| Binding capacity | ~3.5 mg IgG/g resin |
| Dynamic Loading Capacity | ≤100 μg IgG/column at 2 mL/min flow rate |
| pH Range | 1.9–7.5 |
| Maximum Flow rate | Up to 2 mL/min |
| Maximum Pressure | ≤1000 psi |
| Operating Temperature | <30 ?C |
| Data Sheets | |
|---|---|
 | Thermo Scientific MAbPac Protein A Column |
| Manuals | |
|---|---|
| Thermo Scientific MAbPac Protein A Column Quickstart |
| Thermo Scientific MAbPac Protein A Column Product Manual |
| Posters | |
|---|---|
| Protein A Affinity Column for Monoclonal Antibody (MAb) Titer Analysis |
| Applications | |
|---|---|
| A Novel Affinity Protein A Column for Monoclonal Antibody (MAb) Titer Analysis |
| Brochures | |
|---|---|
| Thermo Scientific BioLC Columns |
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