大鼠柠檬酸合酶(CS)检测试剂盒说明上写封闭液用蔗糖溶液,一般是用BSA或酪蛋白的蛋白分子封闭空白位点,蔗糖封闭的原理:
1、大鼠柠檬酸合酶(CS)检测试剂盒实验中,蔗糖本身没有封闭作用;
2、封闭液一般是BSA、酪蛋白等或者是Tween等。
3、加蔗糖的主要目的是保护ELISA板子吸附(包被)的蛋白,起到“保护剂”的作用。
4、蔗糖溶液一般在ELISA板子经过BSA封闭后加,当然也有将蔗糖加到封闭液中同时进行处理;那一种更好需要你实验后来验证。但多数选择前者。
封闭剂还可用5%的脱脂乳(市售即可),0.4%的明胶,但是实验表明前者比较好,后者不容易洗干净未结合上的明胶。
大鼠柠檬酸合酶(CS)检测试剂盒封闭剂的原理就是与酶联板上未结合抗原或抗体的微孔中用封闭剂封闭上,结合抗体或抗原的时候就不会产生非特异结合了,不会影响实验的准确度。
tween的作用:虽然不能单独封闭,但和BSA在封闭时会起到清洗非特异性结合或结合不牢固的蛋白的作用,假如抗体包被在板上会出现YY-
附件:大鼠柠檬酸合酶(CS)检测试剂盒实验中缓冲溶液等试剂的配置
ELISA相关产品,详情见:封闭试剂、终止溶液、封闭肽、ELISA稀释液、ELISA试剂盒、ELISA缓冲液、ELISA洗脱缓冲液
为了保证大鼠柠檬酸合酶(CS)检测试剂盒实验的准确性,实验操作中加入的缓冲液和各组分一定要精确,将洗涤液加入酶标板中的时候防止加入的试剂混入另外一个酶标孔,严防交叉污染。
HZ-EL-RB0109c兔神经营养性酪氨酸激酶2型受体(NTRK2)检测试剂盒Rabbit NTRK2 (Neurotrophic Tyrosine Kinase Receptor Type 2 ) ELISA Kit
HZ-EL-RB0110c兔III型胶原α1(COL3α1)检测试剂盒 Rabbit COL3α1 (Collagen Type III Alpha 1) ELISA Kit
HZ-EL-RB0111c兔乳脂球表皮生长因子8 (MFG-E8)检测试剂盒 Rabbit MFG-E8 (Milk Fat Globule EGF Factor 8) ELISA Kit
HZ-EL-RB0112c兔补体因子4 (C4)检测试剂盒Rabbit C4 (Complement Component 4) ELISA Kit
HZ-EL-RB0113c兔血小板衍生生长因子受体α(PDGFRα)检测试剂盒Rabbit PDGFRα (Platelet Derived Growth Factor Receptor Alpha) ELISA Kit
HZ-EL-RB0114c兔血浆五聚蛋白3 (PTX 3/TSG-14)检测试剂盒Rabbit PTX 3/TSG-14 (Pentraxin 3, Long) ELISA Kit
HZ-EL-RB0115c兔6酮前列腺素(6-K-PG)检测试剂盒 Rabbit 6-K-PG (6-Keto-prostaglandin) ELISA Kit
HZ-EL-RB0116c兔甲状旁腺激素相关蛋白(PTHrP)检测试剂盒Rabbit PTHrP (Parathyroid Hormone Related Protein) ELISA Kit
HZ-EL-RB0117c兔降钙素(CT)检测试剂盒 Rabbit CT (Calcitonin) ELISA Kit
HZ-EL-RB0118c兔纤调蛋白(FMOD)检测试剂盒 Rabbit FMOD (Fibromodulin) ELISA Kit
HZ-EL-RB0119c兔胰蛋白酶原激活肽(TAP)检测试剂盒Rabbit TAP (Trypsinogen Activation Peptide) ELISA Kit
HZ-EL-RB0120c兔分泌型卷曲相关蛋白2 (SFRP2)检测试剂盒Rabbit SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit
HZ-EL-RB0121c兔可溶性半乳糖凝集素3(LGALS3)检测试剂盒Rabbit LGALS3 (Lectin-Galactoside Binding Soluble 3) ELISA Kit
HZ-EL-RB0122c兔凝血因子Ⅸ(F9)检测试剂盒Rabbit F9 (Coagulation Factor Ⅸ) ELISA Kit
HZ-EL-RB0123c兔β-黑色素细胞刺激素(βMSH)检测试剂盒 Rabbit βMSH (beta-Melanocyte Stimulating Hormone) ELISA Kit
HZ-EL-RB0124c兔钙调磷酸酶结合蛋白1(CABIN1)检测试剂盒 Rabbit CABIN1 (Calcineurin Binding Protein 1) ELISA Kit
HZ-EL-RB0125c兔丝裂原活化蛋白激酶14(MAPK14)检测试剂盒Rabbit MAPK14 (Mitogen Activated Protein Kinase 14) ELISA Kit
HZ-EL-RB0126c兔桩蛋白(Pax)检测试剂盒Rabbit Pax (Paxillin) ELISA Kit
HZ-EL-RB0127c兔嗜酸性白血球相关之RNA水解酵素家族成员12(EAR12)检测试剂盒 Rabbit EAR12 (Eosinophil Associated Ribonuclease A family Member 12) ELISA Kit
HZ-EL-RB0129c兔生长分化因子15(GDF15)检测试剂盒Rabbit GDF15 (Growth Differentiation Factor 15) ELISA Kit
HZ-EL-RB0130c兔前列腺素I合酶(PTGIS)检测试剂盒Rabbit PTGIS (Prostaglandin I Synthase) ELISA Kit
HZ-EL-RB0131c兔α1酸性糖蛋白(α1-AGP)检测试剂盒"Rabbit α1-AGP (Alpha-1-Acid Glycoprotein) ELISA Kit
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HZ-EL-RB0132c兔B细胞淋巴瘤因子3(Bcl-3)检测试剂盒 Rabbit Bcl-3 (B-cell Leukemia/Lymphoma 3) ELISA Kit
HZ-EL-RB0133c兔质膜膜泡关联蛋白 (PLVAP)检测试剂盒Rabbit PLVAP (Plasmalemma Vesicle Associated Protein) ELISA Kit
HZ-EL-RB0134c兔血小板衍生生长因子受体样蛋白(PDGFRL)检测试剂盒Rabbit PDGFRL (Platelet Derived Growth Factor Receptor Like Protein) ELISA Kit