美国加利福尼亚大学伯克利分校的科学家,利用单细胞Western技术在期刊SCIENCE ADVANCES(IF:12.804)发表文章:Assessing heterogeneity among single embryos and single blastomeres using open microfluidic design, Sci. Adv. 2020 6: eaay1751 22 April 2020. 本文利用单细胞Western技术与现有的工作流程整合在一起,为评估植入前胚胎发育固有的细胞间分子异质性开辟了新途径。
During biosysnthesis, various post-translational modifi cations (PTMs) will lead to charge heterogeneity in monoclonal antibody (mAb) products. Due to these modifi cations, charge variants can affect the biological properties of mAbs as biotherapeutics. Hence it is very important to monitor this quality attribute during bioprocessing for lot-to-lot consistency and stability. Capillary electrophoresis (CE) with charge to mass based separation mechanism, is best situated for separation of charged molecules. Due to difference in net charge of the charge variants, their electrophoretic mobility differs, resulting in capillary zone electrophoresis (CZE) separation1,2. This Application Note demonstrates the feasibility of using CZE for monitoring the charge variants of innovator and biosimilar rituximab on Agilent 7100 CE system. Further, the basic charge variants of biosimilar rituximab were characterized by CZE/MS.