Acclaim 300 C18 蛋白质和羧氨酸色谱柱
Acclaim 300 C18 蛋白质和羧氨酸色谱柱 蛋白质和羧氨酸的高分辨率分离High-Resolution Separation of Proteins and Peptides概要:The Acclaim 300 features 3 µ m silica particles for rapid analysis of complex protein digests. The Acclaim 300&rsquo s stable bonding make these columns compatible with a wide variety of applications, including LC/MS methods.Designed for high-resolution peptide mapping and protein separationsHigh-efficiency 3 µ m spherical silica substrateHigh-performance bonding chemistry on 300 Å pore silicaApplication tested for suitablility in peptide mappingReproducible for dependable resultsLC/MS compatibleManufacture of these columns starts with ultrapure silica that contains minimal concentrations of metal contaminants, minimizing the tailing effects of residual, exposed silanol groups. Exhaustive bonding and end-capping techniques result in stable bonding and columns that exhibit predictable reversed-phase separations with minimal secondary interactions. The use of a 3 µ m silica particle reduces limits for diffusion of the analytes into the stationary phase, resulting in fast, high-resolution separations. Compared to 5 µ m column packings, the smaller particles support increased flow rates and shallower gradients on shorter columns, for faster separations. Dionex tests each bonded silica lot for peptide selectivity to ensure reproducible performance.规格:Acclaim PepMap for TFA-Free Peptide Mapping SpecificationsColumn typeNanoCapillaryMicroColumn i.d.75 µ m300 µ m1.0 mmLength50, 150, 250, and 500 mm50, 150, and 250 mm50, 150, and 250 mmRecommended Flow Rate200 nL/min4 µ L/min40 µ L/minPacking MaterialC18, 100 Å ,3 µ mC18, 100 Å ,3 µ mC18, 100 Å ,3 µ mC18, 300 Å ,5 µ mC18, 300 Å ,5 µ mC18, 300 Å ,5 µ mC8, 100 Å ,3 µ m C8, 100 Å ,3 µ m C8, 100 Å ,3 µ m C8, 100 Å ,5 µ m C8, 100 Å ,5 µ m C8, 100 Å ,5 µ m C4, 300 Å ,5 µ m C4, 300 Å ,5 µ m C4, 300 Å ,5 µ m ColumnFused-silicaGlass Lined TubingStainless steelColumn Inlet1/16-in.PEEK sleeve adapter(provided)nanoViper (optional)1/16-in.universal fittingnano Viper (optional) 50µ m i.d. PEEK sheathed fused silica with nanoViper fittings1/16-in.PEEK tubingnano Viper (optional) 75µ m i.d. PEEK sheathed fused silica with nanoViper fittingsColumn OutletFused-silica 25-µ m i.d./ 280-µ m o.d.Fused-silica 75-µ m i.d./ 280-µ m o.d1/16-in.PEEK tubing nanoViper (optional) 75µ m i.d. PEEK sheathed fused silica with nanoViper fittingsMS Outlet1-m Length订购信息：The standard particle size is nominally 3 µ m. Analytical columns are available in 2.1, 3.0, and 4.6 mm dimeters standard lengths are 50 and 150 mm. Capillary formats are available in 75 and 300 µ m, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Guard columns in both 2.0 and 4.3 mm sizes are recommended to protect analytical columns.Analytical ColumnsAcclaim 300, C18, 3 µ m Analytical (2.1 x 50 mm)60263Acclaim 300, C18, 3 µ m Analytical (2.1 x 150 mm)60264Acclaim 300, C18, 3 µ m (3.0 x 150 mm)63684Acclaim 300, C18, 3 µ m Analytical (4.6 x 50 mm)60265Acclaim 300, C18, 3 µ m Analytical (4.6 x 150 mm)60266Guard ColumnsAcclaim 300,C18, 3µ m, Guard Cartridges, (2.1 x 10mm), 2 ea requires holder 06958069690PROD,COL,ACCLAIM,300,C18,3UM,4.6X10MM,GRD,2EA69697HardwareAcclaim SST Guard Cartridge Holder V-159456Guard to Analytical Column Coupler59457Guard Kit (Holder and Coupler)59526Acclaim SST Guard Cartridge Holder V-269580Acclaim Guard Kit (Holder and coupler) V-269707Guard to Analytical Column Coupler V-274188Micro and Nano ColumnsAcclaim 300, C18, 3 µ m, 300 Å , 75 µ m i.d. x 5 cm162223Acclaim 300, C18, 3 µ m, 300 Å , 75 µ m i.d. x 15 cm162224Acclaim 300, C18, 3 µ m, 300 Å , 300 µ m i.d. x 5 cm162221Acclaim 300, C18, 3 µ m, 300 Å , 300 µ m i.d. x 15 cm162222Acclaim 120, C18, 3 µ m, 120 Å , 1.0 mm i.d. x 5 cm162219Acclaim 300, C18, 3 µ m, 300 Å , 1.0 mm i.d. x 15 cm162220应用谱图：
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