哈巴俄苷

产品特点：推杆组件，PTFE-Tipped（Hamilton）Replacement Plunger Assembly, PTFE-Tipped (Hamilton)订货号：24919用于Agilent 7670,7671和7672 ALS自动进样器进样针注意：Restek销售的进样针和针头仅供科学研究和实验室使用，不适合人体内使用。产品名称：推杆组件，PTFE-Tipped（Replacement Plunger Assembly, PTFE-Tipped）类似：Agilent 5181-3358,5181-3365体积：10μL终止：N，RN，LT / LTN供应商：Hamilton 供应商Cat. #：13205

9MBA旋转杆品牌：美国PINE型号：AFE 9MBA产地：美国用途：连接工作电极与伺服电机，传输旋转动力参数材质：不锈钢材质外径：15mm 外径配套：原装进口，可连接一个RCE圆柱电极，与参比电极、对电极构建三电极体系。

Horiba EMGA-821/921Nickel capsules for LiquidsDescriptionHoriba #Alpha #Nickel Capsules 11 x 5mm 502-183 Pack of 100905.201.330.001AR2183Nickel Capsules 6.5 x 10mm Pack of 100905.201.060.001AR568Tin capsules for LiquidsDescriptionHoriba #Alpha #Smooth Wall Tin Capsules Round Base 5mm x 18mm Pack of 100905.201-020.001AR2166Chemical ReagentsDescriptionHoriba #Alpha #Copper Oxide Fine Wires 4 x 0.5mm 100g905.120.240.001AEB1002Copper Oxide Fine Wires 4 x 0.5mm 250gAR01039Copper Wires Fine Wires Reduced 4 x 0.5mm 100g905.201.360.001AR189Copper Wires Pound Pack Fine Wires Reduced 4 x 0.5mm 454gAR2304-500AlphaDri Magnesium Perchlorate 501-171 454g905.200.550.001 905.200.550.001ANAR171AlphaSolve A Granular 0.8 to 1.6mm 14 to 25 Mesh 502-174 500g905.200.970.001 905.200.970.001ANAR2174Silica (Quartz) Wool Very Fine 502-177 50g905.201.660.001 905.201.660.001ANAR177Tin Metal Accelerator 501-076 908g905.201.250.001AN 905.201.340.001AR076Tin Metal Accelerator 762-695 11.35 kgAR695Crucibles and BoatsDescriptionHoriba #Alpha #Graphite Crucible Hydrogen Horiba pack of 1000905.200.020.001AR200010Graphite Crucible Hydrogen Horiba pack of 1000905.200.010.001AR200020Inner Crucible Horiba Pack of 1000905.200.050.010Inner Graphite Crucible Hydrogen Horiba Pack of 100905.201.790.001AR79001Nickel Basket 1.5g Each 763-029 Pack of 100AR2345Nickel Basket 1 g Each 763-065 Pack of 100905.201.000.001AR2344Inorganic StandardsDescriptionHoriba #Alpha #See Alpha' s Current List of StandardsO Rings and Operational AccessoriesDescriptionHoriba #Alpha #Silicone Grease 501/241 100g905.200.080.001AR241Wire Brush Bs Wire Flat Type Brush for Dust Removal Horiba905.120.320.001AR14027B

产品名称：Ketone-Based Organic Solvents仪器厂商：Agilent/美国 安捷伦价格：面议库存：是DescriptionUse WithPart No.Organics tubing kit 5 &ndash axialSeaspray nebulizer and double-passcyclonic spray chamber9910125000Organics tubing kit 5 &ndash radialSeaspray nebulizer and double-passcyclonic spray chamber9910125100Organics tubing kit 6 &ndash axialV-groove nebulizer and Sturman-Mastersspray chamber9910125200Organics tubing kit 6 &ndash radialV-groove nebulizer and Sturman-Mastersspray chamber9910125300

Hamilton哈美顿实验室用一次性注射器PP/PE、无橡胶活塞、偏头、无针头QBAA-002013【结构性能】本品注射器由外套和推杆组成，外套采用优质聚丙烯（PP）原料制成，推杆采用聚乙烯（PE）原料制成，化学稳定性好，避免了传统的一次性带活塞性注射器由于推杆上的橡胶与有机溶剂接触时产生的溶质析出而污染样品。本产品可广泛应用于液相、气相和元素分析时样品的前处理，和一次性针头式滤器连用，专门用于色谱分析中样品的过滤和净化。【产品用途】仅供科研实验用，不得用于其他用途！【特殊说明】本注射器不可用于人体，仅限于科研客户实验研究使用。

产品特点：Agilent 6500 系列精确质量数四极杆-飞行 时间液质联用系统* 低于 1 ppm 的 MS 和 2-4 ppm 的 MS/MS 质量准确度提高了小分子鉴定的可信度，降低了蛋白质数据库检索的假阳性率* 埃托摩尔到低飞摩尔的超高灵敏度有助于您分析痕量化合物* 每秒高达 20 张谱图的数据采集速率保证了与快速 LC 和高通量方法的最大兼容性* 25-20,000 m/z 的宽质量范围适用于小分子、多肽或全蛋白的分析* 在 6530 精确质量数四极杆-飞行时间液质联用系统上具有安捷伦的喷雾流技术，为多种应用提供最高的灵敏度, 包括候选药物分析和痕量食品污染物、代谢产物或生物标志物的分析* MassHunter 工作站软件的完全自动化数据分析方法可以充分发掘来自安捷伦四极杆-飞行 时间液质联用系统的精确质量数质谱数据的海量信息订购信息：Agilent 6500 系列精确质量数四极杆-飞行时间液质联用系统说明部件号6520 精确质量数四极杆-飞行时间液质联用系统G6520BA6530 精确质量数四极杆-飞行时间液质联用系统G6530AA

产品名称：Ketone-Based Organic Solvents仪器厂商：Agilent/美国 安捷伦价格：面议库存：是DescriptionNebulizer, Spray ChamberPart No.Organics kit 5 &ndash axialGlass concentric (Conikal) nebulizer, double-passglass cyclonic spray chamber9910127100Organics kit 5 &ndash radialGlass concentric (Conikal) nebulizer, double-passglass cyclonic spray chamber, annealed torch9910127200Organics kit 6 &ndash axialV-groove nebulizer, double-pass Sturman-Mastersspray chamber9910127300Organics kit 6 &ndash radialV-groove nebulizer, double-pass Sturman-Mastersspray chamber, annealed torch9910127400

产品特点：增强型推杆微升进样针（Hamilton）Reinforced Plunger Microliter Syringes (Hamilton)● 加强可延长易碎，小直径推杆的使用寿命。● 经济实惠，因为寿命更长 - 即使在恶劣的应用中也是如此。● 手工搭接的推杆确保与针筒完美密封。可更换RN / R针头。注意：Restek销售的进样针和针头仅供科学研究和实验室使用，不适合人体内使用。订货信息：Reinforced Plunger Microliter Syringes (Hamilton)Catalog #VolumeNeedle TerminationNeedle GaugeNeedle LengthNeedle Point StyleVendor Cat. #Vendor ModelUnits245415 μLN26s2"/51 mm28792095ea.245425 μLRN26s2"/51 mm28792595ea.2454310 μLN26s2"/51 mm280360901ea.2454410 μLRN26s2"/51 mm280370901ea.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。Product Description:bioGenousTMHepatocellular Carcinoma Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human hepatocellular carcinoma organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMHepatocellular Carcinoma Organoid Basal MediumK2105-HCC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMHepatocellular Carcinoma Organoid Supplement B (50x)K2105-HCC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMHepatocellular Carcinoma Organoid Supplement C (250x)K2105-HCC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Hepatocellular Carcinoma Organoid Complete MediumUse a sterile technique to prepare the hepatocellular carcinoma organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Hepatocellular Carcinoma Organoid Supplement B(50x) and Hepatocellular Carcinoma Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Hepatocellular Carcinoma Organoid Supplement B(50x) and 40μL Hepatocellular Carcinoma Organoid Supplement C(250x) to 9.76mL Hepatocellular Carcinoma Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Hepatocellular Carcinoma Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Hepatocellular Carcinoma OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human hepatocellular carcinoma tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 15 min to 45 min. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of hepatocellular carcinoma organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived hepatocellular carcinoma organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥5 times every 1 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 3 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm hepatocellular carcinoma organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMCholangiocarcinoma Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human cholangiocarcinoma organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMCholangiocarcinoma Organoid Basal MediumK2104-LB-A100/A500100mL/500mL4℃，12 monthsbioGenousTMCholangiocarcinoma Organoid Supplement B (50x)K2104-LB-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMCholangiocarcinoma Organoid Supplement C (250x)K2104-LB-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Cholangiocarcinoma Organoid Complete MediumUse a sterile technique to prepare the cholangiocarcinoma organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1. Thaw Cholangiocarcinoma Organoid Supplement B(50x) and Cholangiocarcinoma Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Cholangiocarcinoma Organoid Supplement B(50x) and 40μL Cholangiocarcinoma Organoid Supplement C(250x) to 9.76mL Cholangiocarcinoma Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Cholangiocarcinoma Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Cholangiocarcinoma OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human cholangiocarcinoma tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1.5 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestion mixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 min to let the Matrigel solidify.12.Prepare the required amount of cholangiocarcinoma organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived cholangiocarcinoma organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 5 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm cholangiocarcinoma organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

产品特点：Agilent 6400 系列三重串联四极杆 LC/MS 系统* 飞克级灵敏度，适合各种应用 – 最大的离子化效率和全质量范围的离子传输效率，保证了各种样品分析的最低检测限和定量限* 快速灵敏的多反应监测 (MRM) – 创新的碰撞池设计消除了交叉污染，使得多种成分同时分析成为可能，如食品中的农药或目标蛋白质定量* 自动方法开发和优化软件使得每个组分都具有最佳的参数，从而获得最高的灵敏度* MassHunter 采集软件具有全面数据相关和目标 MS/MS 功能，以及自动多反应监测方法开发软件（MassHunter 优化软件），为 6400 系列三重串联四极杆 LC/MS 提供定期的多反应监测和 21 CFR Part 11 法规遵循支持订购信息：Agilent 6400 系列三重串联四极杆 LC/MS 系统说明部件号6410 三重串联四极杆 LC/MS 系统G6410BA6460 三重串联四极杆 LC/MS 系统G6460AA

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMMouse Liver Ductal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of Mouse ductal organoids(mLDs) derived from adult stem cells. Self-renewal of the ductal epithelium is driven by the proliferation of stem cells and their progenitors located in liver. mLDs display all hallmarks of the ductal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of Mouse liver development and disease, mLDs may also have applications in regenerative biology through ex vivo expansion of the ductal epithelia.技术参数Product Information:ComponentComponent Cat#VolumeStorage& StabilitybioGenousTMMouse Liver Ductal Organoid Basal Medium（Expansion）K2006-MLD -A100/A500100mL/500mL4℃，12 monthsbioGenousTMMouse Liver Ductal Organoid Supplement B(50x)（Expansion）K2006-MLD –B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMMouse Liver Ductal Organoid Supplement C(250x)（Expansion）K2006-MLD –C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Mouse Liver Ductal Organoid Expansion Medium and Maintenance MediumUse sterile technique to prepare the mouse liver ductal organoid expansion medium and maintenance medium. mLDs grown in Mouse Liver Ductal Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. The following examples are for preparing 10 mL of Expansion Medium and Maintenance Medium. If preparing other volumes, adjust accordingly.1.Thaw Mouse Liver Ductal Organoid Supplement B(50x) (Expansion), Mouse Liver Ductal Organoid Supplement C(250x) (Expansion) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.For Mouse Liver Ductal Organoid Expansion Medium. Add 200 μL Mouse Liver Ductal Organoid Supplement B(50x)(Expansion), 40 μL Mouse Liver Ductal Organoid Supplement C(250x) (Expansion) to 10 mL Mouse Liver Ductal Organoid Basal Medium (Expansion). Mix thoroughly.NOTE:If not use immediately, store complete medium at 2-8°C for not more than 2 weeks. bioGenousTMMouse Liver Ductal Organoid Supplement B (Expansion) contains fungicide and antibiotics(50x).Protocol for Establishment of Mouse Liver Ductal OrganoidsEstablishment of Organoids from Primary Tissue1.Collect primary Mouse liver tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Rinse the liver tissuewith Epithelial Organoid Basal Medium(B213151) or DPBSuntil the supernatant is clear.3.Before ducts isolation, thaw Matrigel on ice and keep it cold. Add 5 mL of FBS to 45 mL of Epithelial Organoid Basal Medium to prepare 10% (vol/vol) FBS medium.4.Mince the tissue into small fragments of 5 mm3in a cell culture dish using surgical scissors or scalpels.CRITICALThe dissected samples must be small enough to pass through the tip of a 10 mL pipette.5.Place the dissected pieces of sample into a 15 mL concial tube containing 10 mL of cold Epithelial Organoid Basal Medium with 1% FBS.6.Wash the samples by pipetting with a 10 mL pipette at least ten times.CRITICALFor the subsequent steps, coat the inner surface of every 10 mL pipette with 10% (vol/vol) FBS medium before use to avoid adherence of the samples on the pipette wall.7.Stand the tube still until the samples settle at the bottom. Aspirate the supernatant with a 10 mL pipette and add 10 mL of pre-warmed Tissue Digestion Solution (K601008).8.Digest the tissue fractions in 37℃with rotation at the speed of 200 rpm. The digestion time should not exceed 30 mins.CRITICALTo prevent over-digestion, one should examine under the microscope if the duct structure appear during digestion.9.Once the duct structure appears, stop digestion by transfer the digestion solution into 50mL tube and add 40 ml Epithelial Organoid Basal Medium with 1% FBS.10.Stand the tube for 2 min. Transfer the supernatant into a new tube.11.Spin the supernatant at 4°C at 300g for 3 min. Aspirate and discard the supernatant.12.Re-suspend the pellet with 10 ml Epithelial Organoid Basal Medium with 1%FBS, and spin at 4°C at 300g for 3 min.13.Repeat last step for 2 times.14.Aspirate the supernatant and resuspend the pellet in Matrigel. Matrigel should be kept on ice to prevent it from solidifying thus, work quickly. The amount of Matrigel depends on the size of the pellet. Approximately 20-100 ducts should be plated in 25 μL of Matrigel.CRITICALDo not dilute Matrigel too much (Matrigel should be 70% (Matrigel vol/Total vol)) to ensure formation of solid droplets.15.Plate the Matrigel containing organoids on the bottom of 24-well cell culture plates in droplets of ~30 μL each around the center of the well.CRITICALOnce the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.16.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.17.Prepare the required amount of bioGenousTMMouse Liver Ductal Organoid Expansion Medium.18.Once the Matrigel droplets are solidified (15-25 min), open the plate and carefully add 500 μL of Organoid Complete Medium to each well.CRITICALDo not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.19.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.20.Change the medium every 3 days by carefully aspirating medium from the wells and replacing it with fresh, pre-warmed Organoid Complete Medium.21.Closely monitor the organoid formation. Ideally, Mouse Liver Ductal Organoids should be passaged for the first time between 5 and 8 days after initial plating.Splitting and Passaging of Organoids22.Pipette up and down to disrupt the Matrigel, and transfer the organoid suspension into a 1.5 mL conical tube.23.Pipette the organoid suspension up and down to mix thoroughly.Use the bottom of the tube to create pressure, which will aid the removal of Matrigel.24.Centrifuge organoids at 200g for 3 min at room temperature.25.Aspirate the supernatant, and split organoids using either mechanical disruption or Organoid Dissociation Solution (E238001). For mechanical disruption, resuspend the pellet in 1 mL of Organoid Basal Medium. Use a pipette tip to pipette the organoid suspension up and down 30 times. Use the bottom of the tube to create pressure, which will aid organoid disruption. In case of Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥10 times every 1 min to aid in the disruption of the organoids. Monitor digestion closely to keep the incubation time inOrganoid Dissociation Solution to a minimum.CRITICALDo not dissociate in Organoid Dissociation Solution for 3 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.26.After shearing is complete, wash once by topping up with 1 mL ofOrganoid Basal Medium, and centrifuge at 200g for 3 min at room temperature.27.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets on the bottom of a culture plate as describedin Steps 12. After plating is complete, transfer the plate into ahumidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.28.Pre-warm Mouse Liver Ductal Organoid Maintenance Medium at 37 °C.29.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.30.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

产品名称：Inorganic Anion Solutions Kit仪器厂商：Agilent/美国 安捷伦价格：面议库存：是DescriptionUnitPart No.Inorganic Anion Solutions Kit5063-6511Inorganic anion buffer250 mL8500-6797Ultra pure CE water500 mL5062-85780.1 N sodium hydroxide250 mL5062-85751.0 N sodium hydroxide250 mL5062-8576Bare fused-silica capillary, 50 &mu m ID, 72 cm long2/pkG1600-62211Inorganic anion test mixtureIncludes 1000 ppm each of fluoride, chloride, bromide, nitrite,sulfate and 3000 ppm phosphate10 mL5062-8524

产品特点：Acclaim™ Organic Acid LC 色谱柱使用 Thermo Scientific™ Acclaim™ Organic Acid LC 色谱柱分离亲水、脂族和芳族有机酸可实现前所未有的性能。 这些高效、反相硅胶色谱柱兼容 100% 水流动相，在低 pH 值时拥有卓越的水解稳定性，在分离有机酸时具有出色的选择性。 在快速运行时间内实现高分离度分离和最少的样品制备。高效、高通量有机酸分析为小分子亲水有机酸、C1 至 C7 脂族酸和亲水芳族酸提供理想选择性分析有机酸类时柱效及峰形出色在低 pH 值条件下具有水解稳定性，非常适合有机酸的反相保留经过使用测试，以确保获得一致的亲水性有机酸分离包装在不含金属的 PEEK 柱体内，消除分析物和柱体之间发生有害的相互作用可选粒径，3.0μm 和 5.0μm；可选直径，2.1mm、3.0mm 和 4.0mm；可选长度，150mm 和 250mm推荐用于食品、饮料、制药、化学半成品和环境样品的分析和质量检验。关于 Acclaim Specialty HPLC 色谱柱系列Acclaim 专用色谱柱专为满足具体应用的分离需求而进行开发、制造和测试。 这些色谱柱基于新式、独特的化学结构，可提供出色的分离度和易用性。经过测试可确保一致的分离具有理想选择性，适用于分离特定的靶向分析物出色的峰形其性能经过测试，可确保高质量和可靠性的特定色谱分离Acclaim Organic AcidOptimized and application-tested for the analysis of hydrophilic organic acids* Tested to guarantee consistent hydrophilic organic acid separations *Compatible with 100% aqueous mobile phases* Hydrolytic stability at low-pH conditions*Ideal selectivity for separating a wide spectrum of organic acids* Excellent column efficiency and peak shapes for organic acidsThe Acclaim Organic Acid (OA) is a silica-based reversed-phase column designed for high-efficiency, high-throughput organic acids analysis.It offers unparalleled performance for separating hydroxyl aliphatic and aromatic organic acids.The Acclaim OA is the recommended column for determining small hydrophilic organic acids, C1 to C7 aliphatic acids, and hydrophilic aromatic acid and is also valuable for the analysis and quality assurance of food and beverage products, pharmaceutical preparations, plating baths, and manufacturing chemicals, chemical intermediates, and environmental samples.订购信息：Acclaim Organic AcidParticle Size (μm)FormatLength (mm)2.1mm ID3.0mm ID4.0mm ID3HPLC Column150070087070086–5Guard Cartridges(2/pk)10–071987069700HPLC Column150––062903250––062902Acclaim Guard HolderFormatCat.No.Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188

TSKgel Alpha-4000色谱柱TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble PolymersTSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. Figure 1 shows the calibration curves of all Alpha columns.Figure 1: Calibration Curves of TSKgel Alpha ColumnsUnlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. Suitable solvents are shown in Figure 2.Figure 2: Solvent Compatibility for TSKgel Alpha-3000 for Organic SolventsMixed-bed TSKgel Alpha ColumnThe mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.Base Material:hydrophilic methacylateParticle Size (mean):10 μm Pore size (mean):45 nm Exclusion Limit (Polyethylene oxide - water): 400,000 DaExclusion Limit (Polystyrene - THF):1 million DaShipping SolventwaterMax. Pressure Drop:3 MPa订货信息：TSKgel Alpha-4000色谱柱货号描述粒径材质内径 (mm)长度 (cm)18341TSKgel Alpha-400010 μmStainless Steel7.83018345Guard Column for P/N 001834113 μmStainless Steel64

TSKgel Alpha-6000色谱柱TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble PolymersTSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. Figure 1 shows the calibration curves of all Alpha columns.Figure 1: Calibration Curves of TSKgel Alpha ColumnsUnlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. Suitable solvents are shown in Figure 2.Figure 2: Solvent Compatibility for TSKgel Alpha-3000 for Organic SolventsMixed-bed TSKgel Alpha ColumnThe mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.Base Material:hydrophilic methacylateParticle Size (mean):13 μmPore size (mean):ND Exclusion Limit (Polyethylene oxide - water): 107 Da Exclusion Limit (Polystyrene - THF): 107 DaShipping SolventwaterMax. Pressure Drop:2 MPa订货信息：TSKgel Alpha-6000色谱柱货号描述粒径材质内径 (mm)长度 (cm)18343TSKgel Alpha-600013 μmStainless Steel7.83018345Guard Column for P/N 001834313 μmStainless Steel64

TSKgel Alpha-2500色谱柱TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble PolymersTSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. Figure 1 shows the calibration curves of all Alpha columns.Figure 1: Calibration Curves of TSKgel Alpha ColumnsUnlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. Suitable solvents are shown in Figure 2.Figure 2: Solvent Compatibility for TSKgel Alpha-3000 for Organic SolventsMixed-bed TSKgel Alpha ColumnThe mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.Base Material:hydrophilic methacylateParticle Size (mean):7 μmPore size (mean):2.5 nmExclusion Limit (Polyethylene oxide - Water): 5,000 DaExclusion Limit (Polystyrene - THF):10,000 DaShipping SolventwaterMax. Pressure Drop:4 MPa订货信息：TSKgel Alpha-2500色谱柱货号描述粒径材质内径 (mm)长度 (cm)18339TSKgel Alpha-25007 μmStainless Steel7.83018345Guard Column for P/N 001833913 μmStainless Steel64

TSKgel Alpha-M色谱柱TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble PolymersTSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. Figure 1 shows the calibration curves of all Alpha columns.Figure 1: Calibration Curves of TSKgel Alpha ColumnsUnlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. Suitable solvents are shown in Figure 2.Figure 2: Solvent Compatibility for TSKgel Alpha-3000 for Organic SolventsMixed-bed TSKgel Alpha ColumnThe mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.Base Material:hydrophilic methacylateParticle Size (mean):13 μmExclusion Limit (Polyethylene oxide - water): 107 DaExclusion Limit (Polystyrene - THF): 107 DaShipping SolventwaterMax. Pressure Drop:2 MPa订货信息：TSKgel Alpha-M色谱柱货号描述粒径材质内径 (mm)长度 (cm)18344TSKgel Alpha-M13 μmStainless Steel7.83018345Guard Column for P/N 001834413 μmStainless Steel64

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMBreast Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human breast cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMBreast Cancer Organoid Basal MediumK2147-BC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMBreast Cancer Organoid Supplement B (50x)K2147-BC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMBreast Cancer Organoid Supplement C (250x)K2147-BC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Breast Cancer Organoid Complete MediumUse a sterile technique to prepare the breast cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Breast Cancer Organoid Supplement B(50x) and Breast Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Breast Cancer Organoid Supplement B(50x) and 40μL Breast Cancer Organoid Supplement C(250x) to 9.76mL Breast Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Breast Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Breast Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human breast cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1.5 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well.CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of breast cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived breast cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm breast cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMOvarian Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human Ovarian Cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMOvarian Cancer Organoid Basal MediumK2168-OC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMOvarian Cancer Organoid Supplement B (50x)K2168-OC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMOvarian Cancer Organoid Supplement C (250x)K2168-OC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Ovarian Cancer Organoid Complete MediumUse a sterile technique to prepare the ovarian cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Ovarian Cancer Organoid Supplement B(50x) and Ovarian Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Ovarian Cancer Organoid Supplement B(50x) and 40μL Ovarian Cancer Organoid Supplement C(250x) to 9.76mL Ovarian Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Ovarian Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Ovarian Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human ovarian cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1.5 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of ovarian cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived ovarian cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm ovarian cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMGastric Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human gastric cancer organoid s. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMGastric Cancer Organoid Basal MediumK2179-GC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMGastric Cancer Organoid Supplement B (50x)K2179-GC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMGastric Cancer Organoid Supplement C (250x)K2179-GC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Gastric Cancer Organoid Complete MediumUse a sterile technique to prepare the gastric cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Gastric Cancer Organoid Supplement B(50x) and Gastric Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Gastric Cancer Organoid Supplement B(50x) and 40μL Gastric Cancer Organoid Supplement C(250x) to 9.76mL Gastric Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Gastric Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Gastric Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human gastric cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of gastric cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived gastric cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 5 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm gastric cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

TSKgel Alpha-5000色谱柱TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble PolymersTSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. Figure 1 shows the calibration curves of all Alpha columns.Figure 1: Calibration Curves of TSKgel Alpha ColumnsUnlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. Suitable solvents are shown in Figure 2.Figure 2: Solvent Compatibility for TSKgel Alpha-3000 for Organic SolventsMixed-bed TSKgel Alpha ColumnThe mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.Base Material:hydrophilic methacylateParticle Size (mean):10 μmPore size (mean):100 nmExclusion Limit (Polyethylene oxide - water): 1 million DaExclusion Limit (Polystyrene - THF):7 million DaShipping SolventwaterMax. Pressure Drop:3 MPa订货信息：TSKgel Alpha-5000色谱柱货号描述粒径材质内径 (mm)长度 (cm)18342TSKgel Alpha-500010 μmStainless Steel7.83018345Guard column for P/N 001834213 μmStainless Steel64

TSKgel Alpha-3000色谱柱TSKgel Alpha Columns for the Analysis of Polar Organic-Soluble PolymersTSKgel Alpha columns are composed of spherical, hydrophilic methacrylate beads. The main application area for TSKgel Alpha columns is the analysis of polymers that are soluble in polar organic solvents. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in DMF with 10mmol/L DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in HFIP. Figure 1 shows the calibration curves of all Alpha columns.Figure 1: Calibration Curves of TSKgel Alpha ColumnsUnlike TSKgel PW-type columns, which are stable at most to a 50% organic mixed with water, TSKgel Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. Suitable solvents are shown in Figure 2.Figure 2: Solvent Compatibility for TSKgel Alpha-3000 for Organic SolventsMixed-bed TSKgel Alpha ColumnThe mixed-bed column, TSKgel Alpha-M, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.Base Material:hydrophilic methacylateParticle Size (mean):7 μmPore size (mean):15 nmExclusion Limit (Polyethylene oxide - water): 90,000 DaExclusion Limit (Polystyrene - THF):100,000 DaShipping SolventwaterMax. Pressure Drop:4 MPa订货信息：TSKgel Alpha-3000色谱柱货号描述粒径材质内径 (mm)长度 (cm)18340TSKgel Alpha-30007 μmStainless Steel7.83018345Guard Column for P/N 001834013 μmStainless Steel64

horiba堀场碳硫仪备件消耗品Horiba EMIA-320V2/920V2Chemical ReagentsDescriptionHoriba #Alpha #Copper Metal Accelerator 501-263 3lb905.202.150.001 905.202.150.001ANAR263AlphaCel III Tungsten 763-263 2.5kg905.110.140.001 905.110.140.001ANAR027AlphaDri Magnesium Perchlorate 501-171 454g905.200.550.001 905.200.550.001ANAR171AlphaSolve A Granular 0.8 to 1.6mm 14 to 25 Mesh 502-174 500g905.200.970.001 905.200.970.001ANAR2174Iron Chip Accelerator 501-077 908 g905.110.300.001 905.110.300.001ANAR077Iron Chip High Purity 502-231 454gAR673Iron Chip Standard Grade 763-467 11.35 kgAR467Silica (Quartz) Wool Very Fine 502-177 50g905.201.660.001 905.201.660.001ANAR177Tin Metal Accelerator 501-076 908g905.201.250.001AN 905.201.340.001AR076Crucibles and BoatsDescriptionHoriba #Alpha #Ceramic Crucible 528-018 Pack of 1000905.200.380.001 905.200.380.001ANAR3818Ceramic Crucible (528-018) 528-050 Pack of 500905.202.200.001AR3818BCrucible Cover with 4mm Hole for use with C4500 & C4501 528-040 Pack of 1000905.130.200.001AR040Inorganic StandardsDescriptionHoriba #Alpha #See Alpha' s Current List of StandardsO Rings and Operational AccessoriesDescriptionHoriba #Alpha #Brush 34mm dia. Cylindrical Brush for Dust Removal Pack of 2905.201.210.001AR022BCrucible Tongs 761-929AR929Silicone Grease 501/241 100g905.200.080.001AR241Spoon for Weighing Tungsten905.200.2320.001AR936Tweezer 760-138AR138Wire Brush Bs Wire Flat Type Brush for Dust Removal905.120.320.001AR14027BQuartz, Glassware and Furnace TubesDescriptionHoriba #Alpha #Combustion Tube Transparent Quartz 35mm dia. X 148 Length HF Furnace905.200.560.001ANAR117Crucible Stand Ceramic H50mm905.202.100.001AR5202LE

产品介绍Product Description:bioGenousTMHuman Intestinal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of human intestinal organoids(hIOs) derived from adult stem cells. Self-renewal of the intestinal epithelium is driven by the proliferation of stem cells and their progenitors located in crypts. Human intestinal organoids display all hallmarks of the intestinal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of human intestinal development and disease, human intestinal organoids may also have applications in regenerative biology through ex vivo expansion of the intestinal epithelium.技术参数Product Information:ComponentComponent Cat#VolumeStorage& StabilitybioGenousTMHuman Intestinal Organoid Basal MediumK2002-HI-A100/A500100mL/500 mL4℃，12 monthsbioGenousTMHuman Intestinal Organoid Supplement B(50x)K2002-HI-B100/B5002mL/10 mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMHuman Intestinal Organoid Supplement C(250x)K2002-HI-C100/C5000.4mL/2 mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMHuman Intestinal Organoid Supplement D(250x)K2002-HI-D100/D5000.1mL/0.5 mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Human Intestinal Organoid Expansion Medium and Maintenance MediumUse sterile technique to prepare the human intestinal organoid expansion medium and maintenance medium. hIOs grown in Human Intestinal Organoid Expansion Medium overwhelmingly consisted ofLGR5+stem cells, cycling transit amplifying (TA) cells, early enterocytes and a small number of goblet cells. Organoids grown in Human Intestinal Organoid Maintenance Medium contain LGR5+stem cells, TA cells, early and mature enterocytes, goblet cells, M cells and enteroendocrine cells, as well as a low number of Paneth cells and tuft cells. The following examples are for preparing 10 mL of Expansion Medium and Maintenance Medium. If preparing other volumes, adjust accordingly.1.Thaw Human Intestinal Organoid Supplement B(50x), Human Intestinal Organoid Supplement C(250x) and Human Intestinal Organoid Supplement D(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.For Human Intestinal Organoid Expansion Medium. Add 200 μL Human Intestinal Organoid Supplement B(50x), 40 μL Human Intestinal Organoid Supplement C(250x) and 40 μL Human Intestinal Organoid Supplement D(250x) to 9.72 mL Human Intestinal Organoid Basal Medium. Mix thoroughly.3.For Human Intestinal Organoid Maintenance Medium. Add 200 μL Human Intestinal Organoid Supplement B(50x) and 40 μL Human Intestinal Organoid Supplement C(250x) to 9.76 mL Human Intestinal Organoid Basal Medium. Mix thoroughly.NOTE:If not use immediately, store complete medium at 2-8°C for not more than 2 weeks. bioGenousTMHuman Intestinal Organoid Supplement B contains fungicide and antibiotics(50x).Protocol for Establishment of Human Intestinal OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and governmental regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human intestinal tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium or if they also contain fat or muscle tissue. If so, remove non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissue are present, continue to the next step immediately.3.Rinse the intestinal tissuewith Epithelial Organoid Basal Medium(B213151) or DPBSuntil the supernatant is clear.4.Before crypt isolation, thaw Matrigel on ice and keep it cold. Add 5 mL of FBS to 45 mL of Epithelial Organoid Basal Medium to prepare 10% (vol/vol) FBS medium.5.Mince the tissue into small fragments of 5 mm3in a cell culture dish using surgical scissors or scalpels.CRITICALThe dissected samples must be small enough to pass through the tip of a 10 mL pipette.6.Place the dissected pieces of sample into a 15 mL conical tube containing 10 mL of cold DPBS.7.Wash the samples by pipetting with a 10 mL pipette at least ten times.CRITICALFor the subsequent steps, coat the inner surface of every 10 mL pipette with 10% (vol/vol) FBS medium before use to avoid adherence of the samples on the pipette wall.8.Stand the tube still until the samples settle at the bottom. Aspirate the supernatant with a 10 mL pipette and add 10 mL of cold DPBS.9.Repeat Steps 7 and 8 3–5 times until the supernatant is free of debris.CRITICALThorough washing of the sample is crucial to avoid bacterial contamination.10.Add 10 mL of cold DPBS supplemented with 2.5 mM EDTA (E219121) to the tube. Place the tube on a rocking shaker and rock it gently at 4 °C for 40 min.11.After treatment with EDTA, stand the tube still until the samples settle to the bottom of the tube, and then aspirate the supernatant with a 10 mL pipette and add 10 mL of cold DPBS.12.Stand the tube still until the samples settle at the bottom. Aspirate the supernatant with a 10 mL pipette.13.Add 10 mL of cold DPBS and pipette up and down at least ten times with a 10 mL pipette. Allowed the tissue fragments to settle down under normal gravity for at least 30s. The crypts will be released into the supernatant by pipetting. Place the supernatant containing the isolated crypts into a new 15 mL tube.14.Spin the crypts at 4°C at 400g for 3 min. Remove the supernatant and place the tube on ice.15.Resuspend the pellet in 1 mL of DPBS and transfer the crypt suspension into a new 1.5 mL tube. Drop 20 μL of the crypt suspension on a petri dish. Count the number of crypts under a stereomicroscope and calculate the total number of crypts.16.Spin the crypts at 4°C at 400g for 3 min. Aspirate and discard the supernatant.17.Aspirate the supernatant and resuspend the pellet in Matrigel. Matrigel should be kept on ice to prevent it from solidifying thus, work quickly. The amount of Matrigel depends on the size of the pellet. Approximately 50-250 crypts should be plated in 25 μL of Matrigel.CRITICALDo not dilute Matrigel too much (Matrigel should be 70% (Matrigel vol/Total vol)) to ensure formation of solid droplets.18.Plate the Matrigel containing organoids on the bottom of 24-well cell culture plates in droplets of ~30 μL each around the center of the well.CRITICALOnce the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.19.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.20.Prepare the required amount of bioGenousTMHuman Intestinal Organoid Expansion Medium.21.Once the Matrigel droplets are solidified (15-25 min), open the plate and carefully add 500 μL of Organoid Complete Medium to each well.CRITICALDo not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.22.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.23.Change the medium every 3 days by carefully aspirating medium from the wells and replacing it with fresh, pre-warmed Organoid Complete Medium.24.Closely monitor the organoid formation. Ideally, human intestinal organoids should be passaged for the first time between 5 and 8 days after initial plating.Splitting and Passaging of Organoids25.Pipette up and down to disrupt the Matrigel, and transfer the organoid suspension into a 1.5 mL conical tube.26.Pipette the organoid suspension up and down to mix thoroughly.Use the bottom of the tube to create pressure, which will aid the removal of Matrigel.27.Centrifuge organoids at 200g for 3 min at room temperature.28.Aspirate the supernatant, and split organoids using either mechanical disruption or Organoid Dissociation Solution (E238001). For mechanical disruption, resuspend the pellet in 1 mL of Organoid Basal Medium. Use a pipette tip to pipette the organoid suspension up and down 30 times. Use the bottom of the tube to create pressure, which will aid organoid disruption. In case of Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥10 times every 1 min to aid in the disruption of the organoids. Monitor digestion closely to keep the incubation time inOrganoid Dissociation Solution to a minimum.CRITICALDo not dissociate in Organoid Dissociation Solution for 3 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.29.After shearing is complete, wash once by topping up with 1 mL ofOrganoid Basal Medium, and centrifuge at 200g for 3 min at room temperature.30.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets on the bottom of a culture plate as describedin Steps 12. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.31.Pre-warm Human Intestinal Organoid Maintenance Medium at 37 °C.32.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.33.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2 until the organoids are needed for further experiments.

上海析信为荷兰Prosense公司在中国地区的总代理，可以提供符合各种法规、匹配各品牌厂家的溶出仪的全系列规格耗材配件。我们可提供Agilent, Distek, Erweka, Hanson, Pharmatest, Sotax的溶出度仪转篮，详请如下图： Agilent Compatible BasketsPart No.DescriptoinOEM RefPSBSK010-0110 Mesh Stainless Steel Basket 12-2125PSBSK020-0120 Mesh Stainless Steel Basket 12-2120PSBSK040-0140 Mesh Stainless Steel Basket 12-2100PSBSK040-01G40 Mesh Stainless Steel Basket, Gold coated 12-2105PSBSK040-01PL40 Mesh Precision Length Stainless Ste Basket –PSBSKPTV-4040 Mesh PTFE Coated Basket12-2110PSBSK060-0160 mesh Stainless steel Basket–PSBSK100-01100 Mesh Stainless Steel Basket12-2154PSBSK150-01150 mesh Stainless steel Basket, supported with 20 mesh12-2152PSBSK200-01200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-01400 mesh Stainless steel Basket, sup- ported with 20 mesh–PSBSKSUP-01Suppository Basket, Plastic12-2130 PSSTABSK-10Stationary Basket Assembly with Ba Shaft and 10 Mesh Basket12-2061PSSTABSK-20Stationary Basket Assembly with Ba Shaft and 20 Mesh Basket12-2060PSSTABSK-40Stationary Basket Assembly with Ba Shaft and 40 Mesh Basket12-2063 Agilent Stationary Basket Caleva Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-CA10 Mesh Stainless Steel Basket 0810123PSBSK020-CA20 Mesh Stainless Steel Basket 0810021PSBSK040-CA40 Mesh Stainless Steel Basket 0810019PSBSK150-CA150 Mesh Stainless Steel Basket, supported with 20 mesh–PSBSKUP-CASuppository Basket, Plastic – Distek Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-DK10 Mesh Stainless Steel Basket 2821-0160 PSBSK010-DK 10 Mesh Stainless Steel Basket, Gold coated 2821-0160 -G PSBSK010-DKC 10 Mesh Stainless Steel Basket, Evolution Series 2800-0041 PSBSK020-DK 20 Mesh Stainless Steel Basket 2821-0159 PSBSK020-DKG 20 Mesh Stainless Steel Basket, Gold coated 2821-0159-G PSBSK020-DKC 20 Mesh Stainless Steel Basket, Evolution Series2800-0042 PSBSK040-DK 40 Mesh Stainless Steel Basket2821-0072 PSBSK040-DKG 40 Mesh Stainless Steel Basket, Gold coated2821-0072-G PSBSK040-DKC 40 Mesh Stainless Steel Basket, Evolution Series2800-0032 PSBSKPTD-4040 Mesh PTFE Coated Basket, Evolution2800-0032-TPSBSK060-DKC60 mesh Stainless steel Basket–PSBSK150-DKC150 mesh Stainless steel Basket, supported with 20 mesh2800-0044PSBSK200-DKC200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-DKC400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-DKSuppository Basket, Plastic3250-0445 Electrolab Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-EL10 Mesh Stainless Steel Basket 0103A00054PSBSK020-EL20 Mesh Stainless Steel Basket0103A00055PSBSK040-EL40 Mesh Stainless Steel Basket0103A00057PSBSK040-ELG40 Mesh Stainless Steel Basket, Gold Coated0103A00061PSBSK040-ELD40 Mesh Stainles Steel Basket, "D" -type0103A00177PSBSK100-EL100 Mesh Stainless Steel Basket0103A00060PSBSK150-EL150 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-ELSuppository Basket, Plastic– Erweka Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-EW10 Mesh Stainless Steel Basket90-000-6010 18391PSBSK010-EWG10 Mesh Stainless Steel Basket, Gold coated95-000-6110PSBSK020-EW20 Mesh Stainless Steel Basket90-000-6020 18392PSBSK040-EW40 Mesh Stainless Steel Basket90-000-6040 18393PSBSK040-EWG40 Mesh Stainless Steel Basket, Gold coated90-000-6140PSBSK150-EW150 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-EWSuppository Basket, Plastic70-000-5004 18394 Hanson Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-HR10 Mesh Stainless Steel Basket65-220-010PSBSK010-HRG10 Mesh Stainless Steel Basket, Gold coated–PSBSK020-HR20 Mesh Stainless Steel Basket65-220-020PSBSK040-HR40 Mesh Stainless Steel Basket65-220-000 74-105-252PSBSKPTH-4040 Mesh PTFE Coated Basket–PSBSK040-HRG40 Mesh Stainless Steel Basket, Gold coated–PSBSK060-HR60 mesh Stainless steel Basket–PSBSK150-HR150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-HR200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-HR400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-HRSuppository Basket, Plastic65-700-048 Logan Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-LG10 Mesh Stainless Steel Basket800-0159PSBSK020-LG20 Mesh Stainless Steel Basket800-0155PSBSK040-LG40 Mesh Stainless Steel Basket800-0154PSBSK100-LG100 mesh Stainless steel Basket–PSBSK150-LG150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-LG200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-LG400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-LGSuppository Basket, Palstic800-0170 Pharmatest Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-PT10 Mesh Stainless Steel Basket311-2804PSBSK020-PT20 Mesh Stainless Steel Basket–PSBSK040-PT40 Mesh Stainless Steel Basket311-2801-6PSBSK150-PT150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-PT200 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-PTSuppository Basket, Plastic311-3000-6PSBSK010-PTG10 Mesh Stainless Steel Basket, Gold coated– Sotax Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-ST10 Mesh Stainless Steel Basket–PSBSK010-STG10 Mesh Stainless Steel Basket, Gold coated–PSBSK020-ST20 Mesh Stainless Steel Basket9353PSBSK040-ST40 Mesh Stainless Steel Basket2830PSBSK040-STG40 Mesh Stainless Steel Basket, Gold coated–PSBSK060-ST60 mesh Stainless steel Basket–PSBSK150-ST150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-ST200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-ST400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-STSuppository Basket, Plastic– Zymark Compatible Baskets Part No.DescriptoinOEM RefPSBSK040-ZM40 Mesh Stainless Steel Basket63127PSBSK040-ZM240 Mesh Precision Length Stainless Steel Basket– Dabigatran Etexilate Mesylate The basket is wider than the standard USP basket and therefore it will not fit on to a standard USP1 basket hub and a special hub/basket shaft must be used.Part No.DescriptoinOEM Ref PSBSK040-DEM Dabigatran Etexilate Mesylate, 40 mesh, Stainless Steel –PSBSKSHT-16DEM16" suited for Hanson Research SR6/ SR8–PSSPNBSK-HRDEMSpin On/Off suited for Hanson Vision–PSBSKSHT-DKDEM16.5" suited for Distek–PSBSKSHT-19DEM19" suited for Agilent–PSSPNBSK-01DEMSpin On/Off suited for Agilent–PSBSKSHT-AT7DEMSuited for Sotax AT7–PSBSKHUB-DEMBasket adapter for Distek, DEM– Mini baskets For small volume applications.Part No.DescriptoinOEM RefPSMINBSK-40Universal mini basket 40 mesh–PSMINBSK15-0115" basket shaft, 316S.S., Agilent–PSMINBSK-DK16.5" basket shaft, 316S.S., Distek–PSMINBSK-HR16" basket shaft, 316S.S., Hanson SR6/SR8–PSSPNMINBSK-HRVSpin On/Off basket shaft, 316S.S., Hanson Vision74-105-220PSSPNMINBSK-01Spin On/Off basket shaft, 316S.S., Agilent–溶出度仪取样装置信息由上海析信仪器科技有限公司为您提供，如您想了解更多关于溶出度仪配件报价、型号、参数等信息，欢迎来电或留言咨询！

上海析信为荷兰Prosense公司在中国地区的总代理，可以提供符合各种法规、匹配各品牌厂家的溶出仪的全系列规格耗材配件。我们可提供Agilent, Distek, Erweka, Hanson, Pharmatest, Sotax的溶出度仪转篮，详请如下图： Agilent Compatible BasketsPart No.DescriptoinOEM RefPSBSK010-0110 Mesh Stainless Steel Basket 12-2125PSBSK020-0120 Mesh Stainless Steel Basket 12-2120PSBSK040-0140 Mesh Stainless Steel Basket 12-2100PSBSK040-01G40 Mesh Stainless Steel Basket, Gold coated 12-2105PSBSK040-01PL40 Mesh Precision Length Stainless Ste Basket –PSBSKPTV-4040 Mesh PTFE Coated Basket12-2110PSBSK060-0160 mesh Stainless steel Basket–PSBSK100-01100 Mesh Stainless Steel Basket12-2154PSBSK150-01150 mesh Stainless steel Basket, supported with 20 mesh12-2152PSBSK200-01200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-01400 mesh Stainless steel Basket, sup- ported with 20 mesh–PSBSKSUP-01Suppository Basket, Plastic12-2130 PSSTABSK-10Stationary Basket Assembly with Ba Shaft and 10 Mesh Basket12-2061PSSTABSK-20Stationary Basket Assembly with Ba Shaft and 20 Mesh Basket12-2060PSSTABSK-40Stationary Basket Assembly with Ba Shaft and 40 Mesh Basket12-2063 Agilent Stationary Basket Caleva Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-CA10 Mesh Stainless Steel Basket 0810123PSBSK020-CA20 Mesh Stainless Steel Basket 0810021PSBSK040-CA40 Mesh Stainless Steel Basket 0810019PSBSK150-CA150 Mesh Stainless Steel Basket, supported with 20 mesh–PSBSKUP-CASuppository Basket, Plastic – Distek Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-DK10 Mesh Stainless Steel Basket 2821-0160 PSBSK010-DK 10 Mesh Stainless Steel Basket, Gold coated 2821-0160 -G PSBSK010-DKC 10 Mesh Stainless Steel Basket, Evolution Series 2800-0041 PSBSK020-DK 20 Mesh Stainless Steel Basket 2821-0159 PSBSK020-DKG 20 Mesh Stainless Steel Basket, Gold coated 2821-0159-G PSBSK020-DKC 20 Mesh Stainless Steel Basket, Evolution Series2800-0042 PSBSK040-DK 40 Mesh Stainless Steel Basket2821-0072 PSBSK040-DKG 40 Mesh Stainless Steel Basket, Gold coated2821-0072-G PSBSK040-DKC 40 Mesh Stainless Steel Basket, Evolution Series2800-0032 PSBSKPTD-4040 Mesh PTFE Coated Basket, Evolution2800-0032-TPSBSK060-DKC60 mesh Stainless steel Basket–PSBSK150-DKC150 mesh Stainless steel Basket, supported with 20 mesh2800-0044PSBSK200-DKC200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-DKC400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-DKSuppository Basket, Plastic3250-0445 Electrolab Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-EL10 Mesh Stainless Steel Basket 0103A00054PSBSK020-EL20 Mesh Stainless Steel Basket0103A00055PSBSK040-EL40 Mesh Stainless Steel Basket0103A00057PSBSK040-ELG40 Mesh Stainless Steel Basket, Gold Coated0103A00061PSBSK040-ELD40 Mesh Stainles Steel Basket, "D" -type0103A00177PSBSK100-EL100 Mesh Stainless Steel Basket0103A00060PSBSK150-EL150 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-ELSuppository Basket, Plastic– Erweka Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-EW10 Mesh Stainless Steel Basket90-000-6010 18391PSBSK010-EWG10 Mesh Stainless Steel Basket, Gold coated95-000-6110PSBSK020-EW20 Mesh Stainless Steel Basket90-000-6020 18392PSBSK040-EW40 Mesh Stainless Steel Basket90-000-6040 18393PSBSK040-EWG40 Mesh Stainless Steel Basket, Gold coated90-000-6140PSBSK150-EW150 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-EWSuppository Basket, Plastic70-000-5004 18394 Hanson Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-HR10 Mesh Stainless Steel Basket65-220-010PSBSK010-HRG10 Mesh Stainless Steel Basket, Gold coated–PSBSK020-HR20 Mesh Stainless Steel Basket65-220-020PSBSK040-HR40 Mesh Stainless Steel Basket65-220-000 74-105-252PSBSKPTH-4040 Mesh PTFE Coated Basket–PSBSK040-HRG40 Mesh Stainless Steel Basket, Gold coated–PSBSK060-HR60 mesh Stainless steel Basket–PSBSK150-HR150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-HR200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-HR400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-HRSuppository Basket, Plastic65-700-048 Logan Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-LG10 Mesh Stainless Steel Basket800-0159PSBSK020-LG20 Mesh Stainless Steel Basket800-0155PSBSK040-LG40 Mesh Stainless Steel Basket800-0154PSBSK100-LG100 mesh Stainless steel Basket–PSBSK150-LG150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-LG200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-LG400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-LGSuppository Basket, Palstic800-0170 Pharmatest Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-PT10 Mesh Stainless Steel Basket311-2804PSBSK020-PT20 Mesh Stainless Steel Basket–PSBSK040-PT40 Mesh Stainless Steel Basket311-2801-6PSBSK150-PT150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-PT200 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-PTSuppository Basket, Plastic311-3000-6PSBSK010-PTG10 Mesh Stainless Steel Basket, Gold coated– Sotax Compatible Baskets Part No.DescriptoinOEM RefPSBSK010-ST10 Mesh Stainless Steel Basket–PSBSK010-STG10 Mesh Stainless Steel Basket, Gold coated–PSBSK020-ST20 Mesh Stainless Steel Basket9353PSBSK040-ST40 Mesh Stainless Steel Basket2830PSBSK040-STG40 Mesh Stainless Steel Basket, Gold coated–PSBSK060-ST60 mesh Stainless steel Basket–PSBSK150-ST150 mesh Stainless steel Basket, supported with 20 mesh–PSBSK200-ST200 mesh Stainless steel Basket, supported with 20 mesh–PSBSK400-ST400 mesh Stainless steel Basket, supported with 20 mesh–PSBSKSUP-STSuppository Basket, Plastic– Zymark Compatible Baskets Part No.DescriptoinOEM RefPSBSK040-ZM40 Mesh Stainless Steel Basket63127PSBSK040-ZM240 Mesh Precision Length Stainless Steel Basket– Dabigatran Etexilate Mesylate The basket is wider than the standard USP basket and therefore it will not fit on to a standard USP1 basket hub and a special hub/basket shaft must be used.Part No.DescriptoinOEM Ref PSBSK040-DEM Dabigatran Etexilate Mesylate, 40 mesh, Stainless Steel –PSBSKSHT-16DEM16" suited for Hanson Research SR6/ SR8–PSSPNBSK-HRDEMSpin On/Off suited for Hanson Vision–PSBSKSHT-DKDEM16.5" suited for Distek–PSBSKSHT-19DEM19" suited for Agilent–PSSPNBSK-01DEMSpin On/Off suited for Agilent–PSBSKSHT-AT7DEMSuited for Sotax AT7–PSBSKHUB-DEMBasket adapter for Distek, DEM– Mini baskets For small volume applications.Part No.DescriptoinOEM RefPSMINBSK-40Universal mini basket 40 mesh–PSMINBSK15-0115" basket shaft, 316S.S., Agilent–PSMINBSK-DK16.5" basket shaft, 316S.S., Distek–PSMINBSK-HR16" basket shaft, 316S.S., Hanson SR6/SR8–PSSPNMINBSK-HRVSpin On/Off basket shaft, 316S.S., Hanson Vision74-105-220PSSPNMINBSK-01Spin On/Off basket shaft, 316S.S., Agilent–溶出度仪取样装置信息由上海析信仪器科技有限公司为您提供，如您想了解更多关于溶出度仪配件报价、型号、参数等信息，欢迎来电或留言咨询！

【产品名称】：MID芽孢杆菌鉴定系统（MID-66）【英文名称】：MID Bacillus【产品说明】：MID Bacillus是用于鉴定从食品和食品原料中分离出来的嗜常温芽孢杆菌 (Mesophilic Bacillus spp)的鉴定系统。【储存方式】：未开封的试验条以及芽孢杆菌悬浮肉汤储存于2-8℃条件，可保藏至使用有效期。【产品原理】： MID Bacillus由两条生化反应条组成（标明 BAC 1 和 BAC 2），每条有12个反应小管，小管中装有干燥底物，用于碳水化合物发酵反应和其他反应试验。第二条鉴定条最后一个反应管作为糖发酵空白标准对照。该鉴定条所选择的底物是经过电脑分析选择适用于鉴定和鉴别此菌群的底物进行设计的。 在30℃条件下培养，分别在24、48小时记录结果，并在48小时添加配套试剂（Indole, Nitrate 和 VP tests），记录最后的结果。 将记录结果输入MID鉴定系统软件 (MID-60) 进行分析产品特点： 专为芽孢杆菌属设定的鉴定系统。 鉴定试剂依据传统方法，并已在大量科学论文中得到充分验证 24个生化反应的测试条，操作简单，易于使用 48h获得鉴定结果 使用Mircogen公司的鉴定系统软件分析结果鉴定试剂：铝箔袋独立包装的20套鉴定条（MID66c）（BAC1 和 BAC2）， 20瓶MID66b芽孢杆菌悬浮培养基，反应条培养槽，结果记录报告单，使用说明书。其它必备品：MID鉴定系统软件 (MID-60) 1.1.16.19版本或以上， 矿物油（或无菌液体石蜡油）， VP I 和 VP II 试剂， Nitrate A&B试剂， Kovac试剂， 血琼脂/营养琼脂平板， 革兰氏染液， 触酶反应试剂。鉴定流程:Bacillus ID系统是用于鉴定从食物和食物原料中分离出来的嗜常温芽孢杆菌 (Mesophilic Bacillus spp)。注：了解产品更多资料信息请查阅《MID-66芽孢杆菌鉴定使用说明书（MID Bacillus-ID）》！！！

Acclaim Organic Acid LC ColumnOptimized and Application-Tested for the Analysis of Hydrophilic Organic AcidsThe Acclaim OA column offers unparalleled separations of hydrophilic aliphatic and aromatic organic acids using 100% aqueous mobile phase and at low pH, using UV detection. The Acclaim OA column line features a patented polar-embedded stationary phase that is optimized and use-tested for hydroxyl aliphatic organic acid separations.Use-tested to guarantee consistent hydrophilic organic acid separationsCompatible with 100% aqueous mobile phases, optimum for hydrophilic organic acid retentionHydrolytic stability at low-pH conditions, optimum for reversed-phase retention of organic acidsIdeal selectivity for separating a wide spectrum of organic acidsExcellent peak shapes for organic acidsThe Acclaim OA organic acid columns can be operated at low pH and at 100% aqueous mobile phases, conditions ideal for retaining and separating a wide spectrum of organic acids. Acclaim OA columns undergo extensive testing to ensure column-to-column reproducibility, and are shipped with certificates of analysis detailing these tests. The Acclaim OA columns are packed in metal-free PEEK™ column bodies, to eliminate unwanted interaction between the analytes and the column body.Acclaim OA is the recommended column for determining small hydrophilic organic acids, C1 to C7 aliphatic acids, and hydrophilic aromatic acid and is also valuable for the analysis and quality assurance of food and beverage products, pharmaceutical preparations, plating baths, and manufacturing chemicals and chemical intermediates.Acclaim OA Column ApplicationsApplications include analysis of foods, beverages, pharmaceuticals, chemical intermediates and environmental samples. For example: aliphatic organic acids in foods (juice, wine, drinks), organic acids in drug preparations, acrylic acid and its oligomers, hydroxybenzoic acids, hydroxyphenylacetic acids, arylacetic acids, benzenpolycarboxylic acids and selected amino acids.Acclaim OA Silica-Based Columns for the Separation of Organic Acids SpecificationsParticle size3μm and 5 μmParticle shapeSphericalParticle size distribution (40/90)1.3Total carbon content17%Surface coverage2.7 μmol/m2End-cappedYesMetal impurity (ppm), Na, Fe, Al10.0 ppm Total Metal3.0 ppm Individual MetalPore volume1.0 mL/gAverage pore diameter120 ?Surface area300 m2/gpH range2–8Temperature60 °CStarting materialUltrapure silicaAnalytical ColumnsAcclaim OA, 3μm Analytical, (3.0 x 150mm)070086Acclaim OA, 3μm Analytical, (2.1 x 150mm)070087Acclaim OA, 5 μm Analytical (4 x 250 mm)062902Acclaim OA, 5 μm Analytical (4 x 150 mm)062903Guard ColumnsAcclaim OA, 5 μm Guard Cartridge (4.6 x 10 mm), 2 ea (use V-2 Holder)069700Acclaim OA, 5μm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580071987Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188

Acclaim Organic Acid LC ColumnOptimized and Application-Tested for the Analysis of Hydrophilic Organic AcidsThe Acclaim OA column offers unparalleled separations of hydrophilic aliphatic and aromatic organic acids using 100% aqueous mobile phase and at low pH, using UV detection. The Acclaim OA column line features a patented polar-embedded stationary phase that is optimized and use-tested for hydroxyl aliphatic organic acid separations.Use-tested to guarantee consistent hydrophilic organic acid separationsCompatible with 100% aqueous mobile phases, optimum for hydrophilic organic acid retentionHydrolytic stability at low-pH conditions, optimum for reversed-phase retention of organic acidsIdeal selectivity for separating a wide spectrum of organic acidsExcellent peak shapes for organic acidsThe Acclaim OA organic acid columns can be operated at low pH and at 100% aqueous mobile phases, conditions ideal for retaining and separating a wide spectrum of organic acids. Acclaim OA columns undergo extensive testing to ensure column-to-column reproducibility, and are shipped with certificates of analysis detailing these tests. The Acclaim OA columns are packed in metal-free PEEK™ column bodies, to eliminate unwanted interaction between the analytes and the column body.Acclaim OA is the recommended column for determining small hydrophilic organic acids, C1 to C7 aliphatic acids, and hydrophilic aromatic acid and is also valuable for the analysis and quality assurance of food and beverage products, pharmaceutical preparations, plating baths, and manufacturing chemicals and chemical intermediates.Acclaim OA Column ApplicationsApplications include analysis of foods, beverages, pharmaceuticals, chemical intermediates and environmental samples. For example: aliphatic organic acids in foods (juice, wine, drinks), organic acids in drug preparations, acrylic acid and its oligomers, hydroxybenzoic acids, hydroxyphenylacetic acids, arylacetic acids, benzenpolycarboxylic acids and selected amino acids.Acclaim OA Silica-Based Columns for the Separation of Organic Acids SpecificationsParticle size3μm and 5 μmParticle shapeSphericalParticle size distribution (40/90)1.3Total carbon content17%Surface coverage2.7 μmol/m2End-cappedYesMetal impurity (ppm), Na, Fe, Al10.0 ppm Total Metal3.0 ppm Individual MetalPore volume1.0 mL/gAverage pore diameter120 ?Surface area300 m2/gpH range2–8Temperature60 °CStarting materialUltrapure silicaAnalytical ColumnsAcclaim OA, 3μm Analytical, (3.0 x 150mm)070086Acclaim OA, 3μm Analytical, (2.1 x 150mm)070087Acclaim OA, 5 μm Analytical (4 x 250 mm)062902Acclaim OA, 5 μm Analytical (4 x 150 mm)062903Guard ColumnsAcclaim OA, 5 μm Guard Cartridge (4.6 x 10 mm), 2 ea (use V-2 Holder)069700Acclaim OA, 5μm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580071987Acclaim SST Guard Cartridge Holder V-2069580Acclaim Guard Kit (Holder and coupler) V-2069707Guard to Analytical Column Coupler V-2074188