类器官Human Liver Ductal Organoid Kit (人胆管类器官分化试)
类器官(Organoids)是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致,从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMHuman Liver Ductal Organoid Kit is a chemically defined cell culture medium for establishment and maintenance of human liver ductal organoids(hLDs) derived from adult stem cells. Self-renewal of the liver ductal epithelium is driven by the proliferation of stem cells and their progenitors located in liver. hLDs display all hallmarks of the liver ductal epithelium in terms of architecture, cell type composition, and self-renewal dynamics, therefore hold great promise for unprecedented studies of Human liver development and disease, hLDs could differentiate into hepatocyte like cell under the induction of differentiation medium.技术参数Product Information:ComponentComponent Cat#VolumeStorage& StabilitybioGenousTMHuman Liver Ductal Organoid Basal Medium(Differentiation)K2008-HLH –A100/A500100mL/500mL4℃,12 monthsbioGenousTMHuman Liver Ductal Organoid Supplement B(50x)(Differentiation)K2008-HLH –B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMHuman Liver Ductal Organoid Supplement C(250x)(Differentiation)K2008-HLH –C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMHuman Liver Ductal Organoid Supplement D(250x)(Differentiation)K2008-HLH –D100/D5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Human Liver Ductal Organoid Differentiation MediumUse sterile technique to prepare the human liver ductal organoid differentiation medium. hLDs grown in Human Liver Ductal Organoid Expansion Medium overwhelmingly consisted of cholangiocytes. After changing the Expansion Medium to differentiation medium, the hLDs could differentiate into hepatocyte like cells, which display the markers of hepatocytes, includingALBUMIN,TTRandCYP3A4. The following examples are for preparing 10 mL of Differentiation I Medium and Differentiation II Medium. If preparing other volumes, adjust accordingly.1.Thaw Human Liver Ductal Organoid Supplement B(50x) (Differentiation), Human Liver Ductal Organoid Supplement C(250x) (Differentiation) and Human Liver Ductal Organoid Supplement D(250x) (Differentiation) on ice.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.For Human Liver Ductal Organoid Differentiation Medium I. Add 40 μL Human Liver Ductal Organoid Supplement D(250x) (Differentiation) to 10 mL Human Liver Ductal Organoid Medium(Expansion) (K2008-HLD). Mix thoroughly.3.For Human Liver Ductal Organoid Differentiation Medium II. Add 200 μL Human Liver Ductal Organoid Supplement B(50x) (Differentiation) and 40 μL Human Liver Ductal Organoid Supplement C(250x)(Differentiation)to 10 mL Human Liver Ductal Organoid Basal Medium (Differentiation). Mix thoroughly.NOTE:If not use immediately, store complete medium at 2-8°C for not more than 2 weeks. bioGenousTMHuman Liver Ductal Organoid Supplement B (Differentiation) contains fungicide and antibiotics(50x).