µ-Slide ibiPore Transwell细胞侵袭
货号产品名称规格(个/盒)85016μ-Slide ibiPore培养载玻片,可视化Transwell 0.5μm/20%, ibiTreat底部处理1085026μ-Slide ibiPore培养载玻片,可视化Transwell 3.0μm/5%, ibiTreat底部处理1085036μ-Slide ibiPore培养载玻片,可视化Transwell 5.0μm/5%, ibiTreat底部处理1085046μ-Slide ibiPore培养载玻片,可视化Transwell 8.0μm/5%, ibiTreat底部处理10850?6-S 以上μ-Slide ibiPore培养载玻片,小包装, ibiTreat底部处理2应用:1.流动状态下跨内皮细胞迁移2.2D或者3D凝胶内细胞层共培养和运输3.顶部-基底细胞急性分析4.液体-空气接触的皮肤和肺部模型5.顶部-基底梯度的细胞屏障模型6.基于过滤系统和孔状膜的细胞迁移分析技术特点:1.SiMPore’s G-FLAT? 微孔径玻璃膜2.中间具有玻璃光学膜的跨通道结构3.与盖玻片相比,具有优秀的光学特质4.孔径大小0.5μm,3μm,5μm,8μm5.膜厚度0.3μm(300nm)6.物镜工作距离0.5mm7.完全兼容ibidi泵系统8.特定剪切力大小和剪切力速率水平(详情点击: Application Note 11)Endothelial cells on 3 μm ibiPore membrane. Immunofluorescence overlay image of phase contrast, DAPI (blue), VE-Cadherins (green), F-Actin (red).Fibroblast cells on 3 μm ibiPore membrane. Phase contrast image, objective lens 4x. 应用建议: 孔径 & 孔密度膜类型应用细胞种类案例0.5 μm pores, high porosity (20%)Permeability and transport studies, co-culture models. With this model, cell migration is not possible.Lung cells, epithelial cells3.0 μm pores, low porosity (5%)Transendothelial migrationLeukocytes (neutrophils, T-cells)5.0 μm pores, low porosity (5%)Invasion, migrationMonocytes, macrophages, lymphocytes8.0 μm pores, low porosity (5%)Invasion, migrationTumor and cancer cells, endothelial and epithelial cells, fibroblasts, osteoblasts, melanoma, glioma越大的细胞 – 建议使用越大的孔径。 不同应用的建议孔径:μ-Slide ibiPoreμ-Slide ibiPore 细胞侵袭载玻片结构交叉通道结构和中间ibiPore膜结构的3D示意图应用实例扫描电镜下的多孔玻璃膜 3微米孔 0.5微米孔