提高当前的测序效率,备以今后的规模需求查看NextSeq 1000/1000-CN和NextSeq 2000/2000-CN测序系统的规格,从零开始重新设计,满足不断发展的需求所有系统 /测序平台 /NextSeq 1000/1000-CN和NextSeq 2000/2000-CN:每个流动槽的测序产出*NextSeq 1000/1000-CN和NextSeq 2000/2000-CN流动槽类型P1P2P3 &dagger 1 × 50 bp60 Gb2 × 50 bp10 Gb40 Gb120 Gb2 × 100 bp80 Gb240 Gb2 × 150 bp30 Gb120 Gb360 Gb2 × 300 bp60 Gb180 Gb(300 M)* 输出规格基于单个流动槽,使用Illumina PhiX对照文库,在支持的簇密度下产出进行计算。&dagger P3试剂仅适用于NextSeq 2000/2000-CN系统。保质期NextSeq 1000/1000-CN和NextSeq 2000/2000-CN条目储存温度保质期卡盒-25°C至-15°C6个月流动槽2°C至8°C6个月RSB(含Tween 20)*2°C至8°C6个月* 室温下运输每个流动槽通过过滤的readNextSeq 1000/1000-CN和NextSeq 2000/2000-CN流动槽类型P1P2P3 *单端reads100M400M &dagger 1.2B双端reads200M800M &Dagger 2.4B* P3试剂仅适用于NextSeq 2000/2000-CN系统。&dagger 对于2 × 300 bp的测序深度,为300M单端reads。&Dagger 对于2 × 300 bp的测序深度,600M双端reads。质量分值*与运行时间&dagger NextSeq 1000/1000-CN和NextSeq 2000/2000-CN流动槽类型P1P2P3 &Dagger 质量分值 1 × 50 bp在1 × 50 bp时,≥ 90%的碱基分值高于Q302 × 50 bp在2 × 50 bp时,≥ 90%的碱基分值高于Q302 × 100 bp在2 × 100 bp时,≥ 85%的碱基分值高于Q302 × 150 bp在2 × 150 bp时,≥ 85%的碱基分值高于Q302 × 300 bp在2 × 300 bp时,≥ 80%的碱基分值高于Q30运行时间 1 × 50 bp约11小时2 × 50 bp约10小时约13小时约19小时2 × 100 bp约21小时约33小时2 × 150 bp约19小时约29小时约48小时2 × 300 bp约34小时约44小时* 质量分值(Q值)是对碱基检出中错误发生几率的预测。>Q30的碱基百分比根据整个运行中的数据取平均值。质量分值是基于NextSeq试剂盒在NextSeq 1000/1000-CN和NextSeq 2000/2000-CN系统上的运行所计算的,利用了Illumina PhiX对照文库。根据文库类型和质量、插入片段大小、上样浓度及其他实验因素的不同,性能表现可能有所差异。&dagger 运行时间包括NextSeq 1000/1000-CN或NextSeq 2000/2000-CN系统上的簇生成、测序和碱基检出。&Dagger P3试剂仅适用于NextSeq 2000/2000-CN系统。对关键应用估计的样本通量*NextSeq 1000/1000-CN和NextSeq 2000/2000-CN流动槽类型P1P2P3 &dagger 小型全基因组测序(300个循环)130 Mb基因组;覆盖度大于30倍~7~30~90小型全基因组测序(600个循环)130 Mb基因组;覆盖度大于30倍~12~36~ 全外显子组测序(200个循环)平均目标区域覆盖度为50倍;90%的目标区域覆盖度为20倍~4&Dagger ~16~48总RNA-Seq(200个循环) §每个样本50M read对~2&Dagger ~16~24mRNA-Seq(200个循环)§每个样本25M read对~4&Dagger ~32~48单细胞RNA-Seq(100个循环)**5K细胞,20K reads/细胞1&dagger &dagger ~4~11miRNA-Seq或小RNA分析(50个循环)11M reads/样本~9&Dagger &Dagger 36§§~10816S RNA测序(600个循环)~384***~384***~ * 表格数据代表每次运行预计样本数。&dagger P3试剂仅适用于NextSeq 2000/2000-CN系统。&Dagger 使用P1 300个循环试剂盒。§ 对于Illumina Stranded Total RNA Prep和Illumina Stranded mRNA Prep,建议读长是2 × 75 bp,对于Illumina RNA Prep with Enrichment建议读长是2 × 100 bp。** 建议的测序深度在很大程度上取决于样本类型和实验目标,需要针对每项研究进行优化。&dagger &dagger P1试剂是单细胞质量控制实验的好选择。&Dagger &Dagger 使用P1 100个循环试剂盒。§§ 使用P2 100个循环试剂盒。*** 因美纳提供最多384个唯一双标签序列。Sequencing output per flow cellaNextSeq 1000 and NextSeq 2000Flow cell typeP1bP2bP3b,cP4c1 × 50 bp90 Gb2 × 50 bp10 Gb40 Gb120 Gb180 Gb2 × 100 bp80 Gb240 Gb360 Gb2 × 150 bp30 Gb120 Gb360 Gb540 Gb2 × 300 bp60 Gb240 Gba. Output specifications are based on an Illumina PhiX control library at supported cluster densities.b. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.c. P3 and P4 reagents are available for the NextSeq 2000 System only.Shelf lifeNextSeq 1000 and NextSeq 2000ItemStorage TemperatureP1, P2, P3 Shelf LifeaP4 Shelf LifebCartridge-25°C to -15°C6 months3 monthsFlow Cell2°C to 8°C6 months3 monthsRSB with Tween 20c2°C to 8°C6 months6 monthsa. P1, P2, and P3 with XLEAP-SBS chemistry will launch in Q2 of 2024 with a shelf life of 6 months.b. In 1H of 2024, P4 shelf life will be 3 months. This will be extended to 6 months in 2H of 2024.c. Shipped at room temperature.Reads passing filter per flow cellNextSeq 1000 and NextSeq 2000 with XLEAP-SBS chemistryFlow Cell TypeP1aP2aP3a,bP4bSingle-end Reads100M400M c1.2B1.8BPaired-end Reads200M800M d2.4B3.6Ba. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.b. P3 and P4 reagents are available for the NextSeq 2000 System only.c. 300M single-end reads for 2 × 300 bp with standard SBS chemistry. 400M single-ends reads for 2 x 300 bp with XLEAP-SBS chemistry.d. 600M paired-end reads for 2 × 300 bp with standard SBS chemistry. 800M single-ends reads for 2 x 300 bp with XLEAP-SBS chemistry.Quality scores and run timeNextSeq 1000 and NextSeq 2000 with XLEAP-SBS chemistryFlow cell typeP1aP2aP3a,bP4bQuality scoresc1 × 50 bp≥ 90% of bases higher than Q302 × 50 bp≥ 90% of bases higher than Q302 × 100 bp≥ 90% of bases higher than Q302 × 150 bp≥ 90% of bases higher than Q302 × 300 bp≥ 85% of bases higher than Q30Run timed1 × 50 bp12 hr2 × 50 bp8 hr12 hr18 hr20 hr2 × 100 bp19 hr31 hr34 hr2 × 150 bp17 hr22 hr40 hr44 hr2 × 300 bp34 hr42 hra. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.b. P3 and P4 reagents are available for the NextSeq 2000 System only.c. Quality scores are based on an Illumina PhiX control library performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.d. Run time includes cluster generation, sequencing, and base calling on a NextSeq 1000 or NextSeq 2000 System.Estimated sample throughput for key applicationsaNextSeq 1000 and NextSeq 2000 with XLEAP-SBS chemistryFlow Cell TypeP1bP2bP3b,cP4cSmall Whole-Genome Sequencing (300 cycles)130 Mb genome 30× coverage73092138Whole-Exome Sequencing (200 cycles)~8 Gb per exome, 90× mean coverage~2d103045Total RNA-Seq (200 cycles)50M read pairs per sample2d82436mRNA-Seq (200 cycles)25M read pairs per sample4d164872Single-Cell RNA-Seq (100 cycles)5K cells, 20K reads/sample1e41248miRNA-Seq or Small RNA Analysis (50 cycles)11M reads/sample9f36f108f16316S RNA sequencing (600 cycles)384g384ga. Table values represent estimated number of samples per run. Recommended sequencing depth will largely depend on sample type and experimental objective and will need to be optimized for each study.b. XLEAP-SBS reagents for P1, P2, and P3 flow cells available Q2 of 2024.c. P3 and P4 reagents are available for the NextSeq 2000 System only.d. Use P1 300-cycle kit.e. P1 reagents are a good option for single-cell quality control experiments.f. Use 100-cycle kits.g. A maximum of 384 unique dual indexes is available from Illumina. NextSeq 1000/1000-CN和NextSeq 2000/2000-CN仪器规格表仪器配置具有集成式DRAGEN Bio-IT FPGA二级分析的独立干燥仪器仪器控制计算机操作环境激光尺寸包装尺寸电源要求网络连接带宽产品安全与合规安全性:1级激光产品NRTL认证IEC61010-1 CE认证FCC/IC批准内部网络上传速度:200 Mb/s/仪器BaseSpace Sequence Hub上传速度:200 Mb/s/仪器仪器操作数据上传速度:5 Mb/s/仪器仪器输入电压:100 V到240 V交流电仪器输入频率:50/60 Hz包装宽×深×高:92 cm × 120 cm × 118 cm毛重:232 kg宽x深x高:60 cm × 65 cm × 60 cm重量:141 kg波长:449 nm、523 nm、820 nm温度:15°C-30°C湿度:相对湿度20%–80%,非冷凝海拔:0–2000米仅供室内使用主机:位于仪器内部的2U微型服务器内存:288 GB硬盘:3.8 TB SSD操作系统:Linux CentOS 7.6
留言咨询