能量过滤式透射显微镜

为了促进国内分析电镜技术的普及， 介绍电子能量损失谱仪硬件及应用发展， 美国 Gatan 公司与日本电子公司将与 12 月 2 日在上海交通大学举办能量过滤分析电镜技术讲座。 本次讲座特别针对能量损失谱仪（Electron Energy Loss Spectrometer--EELS） 技术 及透射电子显微镜技术， 邀请了美国 Gatan 公司、 日本电子株式会社（JEOL Ltd.） 的专 业技术人员， 向大家介绍电子能量损失谱仪及分析型电镜的先进技术， 将最新的信息分享 给国内的电镜用户。 除了国外专家分享电镜技术的发展现状之外， 我们还安排了现场上机演示环节。 包括能量 过滤元素面分布（EFTEM）、 扫描透射(Scanning Transmission Electron Microscopy, STEM)模式下利用 High-Speed Spectrum Imaging 与 DualEELS 技术进行成分鉴定及 物相分析等先进技术。上海交通大学购买了两台日本电子的球差校正透射电镜JEM-ARM200F,冷场发射和热场发射各一台，分别安装了聚光镜和物镜球差校正器，都配置了Gatan的EELS系统。

据JEOL USA消息, 美国德克萨斯大学埃尔帕索校区(UTEP)购买日本电子株式会社最新型300kV高衬度能量过滤冷冻透射电镜(cryo-TEM)JEM-3200FS用于结构生物学研究。包括日本电子株式会社最新开发的病毒自动分析软件(JADAS)。　　项目负责人是结构生物学家Dr. Ricardo Bernal and Dr. Chuan “River” Xiao：研究方向为单颗粒重构和断层扫描显微分析(single particle reconstructions and tomography)，该研究组还将同时引进大分子NMR，大分子x射线衍射仪等大型设备。　　使用冷冻透射电镜JEM-3200FS的研究包括测定感染并杀死细菌的噬菌体和病毒的结构，从而找到因细菌耐药性而失去效力的抗生素替代品。还将研究引起人类50%以上感冒的小核糖核酸结构/受体复合物。附：December 16, 2009 (Peabody, Mass.) -- The University of Texas El Paso has selected a JEOL cryo-electron microscope for its emerging structural biology program to be housed in a new building planned for completion in 2011 on the UTEP campus. The JEM-3200FS that was ordered from JEOL USA is a 300 keV cryo-TEM with an omega energy filter for increased contrast and high resolution. The UTEP research program directed by structural biologists Dr. Ricardo Bernal and Dr. Chuan “River” Xiao, will utilize cryo-electron microscopy for single particle reconstructions and tomography. “This 300 keV field emission gun TEM will provide all the capabilities we need to establish a world class facility at UTEP,” Dr. Bernal said. “Our goal is to set up a structural biology program that includes macromolecular NMR, macromolecular X-ray crystallography, and cryo-EM.” Research projects for the new JEOL cryo-TEM include the structure determination of bacteriophages, viruses that infect and kill bacteria, in an effort to find alternatives to antibiotics which are losing their effectiveness due to bacterial resistance. The TEM will also be used to analyze the structure of picornavirus/receptor complexes, responsible for more than 50 percent of all human common colds. Other projects include the structure of ATPases and chaperonins. The expertise of Drs. Bernal and Xiao has resulted in collaborations on more than 12 research projects involving nine researchers across four departments and two universities. Research projects for the new JEOL cryo-TEM include the structure determination of bacteriophages, viruses that infect and kill bacteria, in an effort to find alternatives to antibiotics which are losing their effectiveness due to bacterial resistance. The TEM will also be used to analyze the structure of picornavirus/receptor complexes, responsible for more than 50 percent of all human common colds. Other projects include the structure of ATPases and chaperonins. The expertise of Drs. Bernal and Xiao has resulted in collaborations on more than 12 research projects involving nine researchers across four departments and two universities. In one of the largest instrumentation grants awarded to UTEP, the National Science Foundation will fund the purchase of the new high resolution, high performance cryo-TEM, which will be shipped from JEOL, Ltd. in Akashima, Japan. The JEM-3200FS will include an optional JEOL Automated Data Acquisition System (JADAS) specially developed for cryo-electron microscopy, and a high tilt tomography cryo-transfer system. The JEM-3200FS combines high resolution and 0-loss sample imaging with analytical performance in a 300kV class analytical TEM. The modular nature of the JEM-3200FS will allow easy adaptation to emerging and promising techniques, such as phase-plate microscopy using Zernike and electro-static phase plates. “This is one of the JEOL high end TEMs that is gaining widespread use in the U.S. specifically for cryo-tomography. We have worked closely with customers to develop more of the features structural biologists need,” said Dr. Thomas Isabell, Director of TEM at JEOL USA

中国科学院长春应用化学研究所高分子物理与化学国家重点实验室近期购买了日本电子株式会社（JEOL）的极低温透射电子显微镜JEM-3200FSC。该设备是世界顶级产品，其显著特点是①样品台具有双层冷冻保温设计，最内层的部分可灌入液氦，可在液氦温度下实现透射电镜的极限观察，最大程度地减小了电子束的热损伤效应；②独特的欧米伽能量过滤器可以把影响图像衬度的能量损失电子过滤掉，从而大大地提高样品的衬度和信息量，这些都是日本电子株式会社为高端研究开发的独有技术。 中国科学院长春应用化学研究所是集基础研究，应用研究和高技术创新研究为一体的综合性化学研究所，在国内外具有很高的影响力。该所的高分子物理与化学国家重点实验室定位于高分子科学的基础研究和高分子材料的高技术研究，选择高分子的高性能化、高分子复杂体系和功能高分子的分子工程为主要研究方向，以建设具有国际一流水平的高分子科学研究平台为目标。近年来，他们在高分子科学基础和应用基础研究方面取得了在国际上具有原创性的研究成果。不仅如此，在高分子材料应用研发领域，他们还获得了一批具有自主知识产权、有国际竞争力的重要成果，其中一些在产业化方面取得显著成效。在“国家化学学科重点实验室评估”中连续五次被评为优秀实验室。高分子样品的共同特点与生物样品很类似，都是由轻元素构成，经不住电子束的轰击和热损伤，图像衬度极差，为解决此类问题，必须对电子显微镜进行有针对性地设计和改进，而日本电子株式会社制造的JEM-3200FSC极低温透射电子显微镜正是突破了这些限制。JEM-3200FSC的引进标志着中国的冷冻电镜达到世界领先水平，为高分子材料的研究提供了一个新思路、高手段和高平台，必将进一步促进我国相关研究领域科研大发展。