MC1000 8通道藻类培养与在线监测系统 MC1000 8通道藻类培养与在线监测系统由8个100ml藻类培养试管、水浴控温系统、LEDs光源控制系统及光密度和溶解氧(选配)在线监测系统等组成,可用于藻类培养与控制实验、梯度对比实验等,适于水体生态毒理学研究检测、藻类生理生态研究、水生态研究等,其主要功能特点如下:1. 8通道藻类培养,每个藻类培养试管可培养85ml藻液2. LEDs光源,可对每个培养试管独立调节控制和设置光强度和时间,如昼夜变化等3. 光密度在线监测,包括OD680、OD720,监测数据自动存储4. 溶解氧在线监测(备选)以测量分析藻类光合作用等5. 温度、光照控制可用户设置不同的程序模式6. 气泡混匀:可通过调节阀手动调节气流量以对培养试管内的藻类进行混匀7. 可选配O2/CO2监测系统,在线监测藻类光合放氧和CO2吸收8. 可选配藻类荧光测量模块应用领域:l 多通道同步藻类培养l 同步梯度胁迫实验l 培养条件优化l 控制培养条件与藻类生长动力学监测仪器型号:MC 1000-OD: 8个通道光源颜色相同,标配冷白光LEDMC 1000-OD-WW:8个通道光源颜色相同,标配暖白光LEDMC 1000-OD-MULTI: 8个通道光源颜色不同,分别为1)紫光405nm,2)品蓝光450nm,3)蓝光470nm,4)暖白光,5)绿光540nm,6)黄橙光590nm,7)深红光660nm,8)远红光730nmMC 1000-OD-MIX:每个通道可配备8种不同颜色的LED光源,LED颜色为1)紫光405nm,2)品蓝光450nm,3)蓝光475nm,4)2个暖白光LED,5)绿光530nm,6)橙红光615nm,7)深红光660nm,8)远红光730nm技术指标:1. 藻类同步培养通道:8个2. 培养管容量:100ml,建议最大培养容量85ml3. 在线即时监测参数:分别监测每个培养管的OD680和OD720,数据自动保存到主机内存中,PIN光电二极管检测器,665-750nm带通滤波器4. 精确控温范围:标准配置高于环境温度5-10℃(与光强有关)~60℃,可选配15℃-60℃(环境温度20℃,需加配制冷单元)5. 加热系统:150W筒形加热器,水浴控温6. 水浴体积:5L7. 水浴自动补水模块(选配):水浴水位因蒸发降低后可自动补水8. 光源系统:全LED光源,可在0-100%范围内调控,每个通道的光强可分别独立调控1) MC 1000-OD:标配冷白光LED,可选配暖白光、红光(635nm)或蓝光(470nm)LED;光强0-1000μmol/m2/s可调, 可升级至0-2500μmol/m2/s2) MC 1000-OD-WW:标配暖白光LED,光强0-1000μmol/m2/s可调,更高光强可定制3) MC 1000-OD-MULTI:8个通道光源颜色不同,分别为紫光405nm,品蓝光450nm,蓝光470nm,暖白光,绿光540nm,黄橙光590nm,深红光660nm,远红光730nm;光强0-1000μmol/m2/s可调4) MC 1000-OD-MIX:每个通道可配备8种不同颜色的LED光源,最大光强可达2500μmol/m2/s9. 控光模式:可静态或动态设置光照程序,如正弦、昼夜节律、脉冲等10. 控制单元显示屏:可调控培养程序和显示数据11. 气流调控:通过多管调节阀对8个培养管手动独立调控气体流量12. OD测量程序:将主机内存中的OD数据下载到电脑中并以图表形式显示,数据可导出为TXT或Excel文件13. MC实时在线监测分析模块(含专用工作站和软件基础版或高级版,选配)1) 同时控制2台MC1000(基础版)或无限台MC1000(高级版)2) 通过PBR软件动态调控光照和温度模式3) 通过光密度(OD680、OD720)变化实时监测藻类生物量4) 对生长速率进行实时回归分析5) 多数据管理功能(过滤、查找、多重导出)6) 可将测量数据、培养程序和其他信息保存到数据库中7) 通过GUI图形用户界面设置培养程序并在线显示测量数据图8) 数据可导出为CSV文件9) 支持GMS高精度气体混合系统(仅限高级版)10) 用户自编程培养程序(仅限高级版)11) 设定实验起始时间(仅限高级版)12) 电子邮件通知(仅限高级版)14. GMS150高精度气体混合系统(选配):可控制气体流速和成分,标配为控制氮气/空气和二氧化碳,气源需用户自备15. 恒浊控制模块(选配):带有8个控制阀,可独立控制8个培养管的浊度,由软件自动控制 16. O2/CO2监测系统(选配):8通道续批式监测藻类CO2吸收或光合放氧通量:1) 氧气分析测量:氧气测量范围0-100%,分辨率0.0001%,精确度优于0.1%,温度、压力补偿,数码过滤(噪音)0-50秒可调,具两行文字数字LCD背光显示屏,可同时显示氧气含量和气压2) 二氧化碳分析测量:双波长非色散红外技术,测量范围0-5%或0-15%两级选择(双程),分辨率优于0.0001%或1ppm(可达0.1ppm),精确度1%,通过软件温度补偿,具两行文字数字LCD背光显示屏,可同时显示CO2含量和气压,具数码过滤(噪音)功能3) 气体抽样与气路切换:具备隔膜泵、气流控制针阀和精密流量计,气路自动定时切换功能17. 藻类荧光测量模块(选配):用于测量藻类荧光参数以反映藻类生理状态及浓度,荧光测量程序包括Ft,QY,OJIP-test,NPQ、光响应曲线等,可选配探头式测量或试管式测量:1) 探头式测量:具备光纤测量探头,可插入培养液中原位测量藻类荧光参数 2) 试管式测量:具备测量杯,可取样精确测量藻类荧光参数及光密度值18. 通讯方式:USB19. 尺寸:71×33×21 cm20. 重量:13kg21. 供电:110-240V应用案例:莱茵衣藻全基因组重测序的样品预培养与生长动态监测(Flowers, 2015, Plant Cell)通过基因工程改造莱茵衣藻控制生物污染(Loera-Quezada, 2016, Plant Biotechnology Journal)产地:捷克参考文献:1. Barera S, et al. 2021. Effect of lhcsr gene dosage on oxidative stress and light use efficiency by Chlamydomonas reinhardtii cultures. Journal of Biotechnology 328: 0168-1656.2. Pivato M, et al. 2021. Heterologous expression of cyanobacterial Orange Carotenoid Protein (OCP2) as a soluble carrier of ketocarotenoids in Chlamydomonas reinhardtii. Algal Research 55(16):102255.3. Gachelin M, et al. 2021. Enhancing PUFA-rich polar lipids in Tisochrysis lutea using adaptive laboratory evolution (ALE) with oscillating thermal stress. Applied Microbiology and Biotechnology 105: 301-312.4. Chen H, et al. 2021. A Novel Mode of Photoprotection Mediated by a Cysteine Residue in the Chlorophyll Protein IsiA. mBio 12(1).5. Cecchin M, et al. 2021. CO2 supply modulates lipid remodelling, photosynthetic and respiratory activities in Chlorella species 18(2): 431842.6. Dixit RB, et al. 2021. Secretomics: A Possible Biochemical Foot Printing Tool in Developing Microalgal Cultivation Strategies. doi: 10.21203/rs.3.rs-163118/v17. Kareya MS, et al. 2020. Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587. Frontiers in Plant Science 11: 981.8. Billey E, et al. 2021. Characterization of the Bubblegum acyl-CoA synthetase of Microchloropsis gaditana. Plant Physiology 185(3): 815-835.9. Vonshak A, et al. 2020. Photosynthetic characterization of two Nannochloropsis species and its relevance to outdoor cultivation. Journal of Applied Phycology 32(2):909-922.10. Dienst D, et al. (2020). High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803. Scientific Reports 10(1): 5932.11. Weiner I, et al. 2020. CSO -A sequence optimization software for engineering chloroplast expression in Chlamydomonas reinhardtii. Algal Research 46: 101788.12. Akma C, et al. 2020. Two-phase method of cultivating Coelastrella species for increased production of lipids and carotenoids. Bioresource Technology Reports 9: 100366.13. Cecchin M, et al. 2020. Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants. Biotechnology for Biofuels 13(1): 78.14. Alvarenga D, et al. 2020. AcnSP – A Novel Small Protein Regulator of Aconitase Activity in the Cyanobacterium Synechocystis sp. PCC 6803. Frontiers in Microbiology 11: 1445.15. Zhang B, et al. 2020. The carbonate concentration mechanism of Pyropia yezoensis (Rhodophyta): evidence from transcriptomics and biochemical data. BMC Plant Biology 20(1): 424.16. Nzayisenga, JC, et al. 2020. Effects of light intensity on growth and lipid production in microalgae grown in wastewater. Biotechnology for Biofuels 13(284): 1179-1184.17. Cecchin M, et al. 2020. Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants. Biotechnology for Biofuels 13(6): 312. 18. Flamholz AI, et al. 2020. Functional reconstitution of a bacterial CO2 concentrating mechanism in Escherichia coli. eLife 9: e59882.19. Gupta JK, et al. 2020. Overexpression of bicarbonate transporters in the marine cyanobacterium Synechococcus sp. PCC 7002 increases growth rate and glycogen accumulation. Biotechnology for Biofuels 13: 17. 20. Valev D, et al. 2020. Testing the Potential of Regulatory Sigma Factor Mutants for Wastewater Purification or Bioreactor Run in High Light. Current Microbiology 77(8) : 1590-1599.21. Yao L, et al.. 2020. Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes. Nature Communications 11(1): 1666.22. Shrameeta S, et al. 2020. Glycogen Metabolism Supports Photosynthesis Start through the Oxidative Pentose Phosphate Pathway in Cyanobacteria1. Plant Physiology 182(1):507-517.23. Alessandra B, et al. 2020. Photosynthesis Regulation in Response to Fluctuating Light in the Secondary Endosymbiont Alga Nannochloropsis gaditana. Plant & Cell Physiology 61(1): 41-52..
留言咨询