活体基因导入仪

活体转基因--钳状电极用于体内药物/基因/蛋白质传递及基因治疗电极．活体转基因--钳状电极Tweezertrodes是一种可复用的小钳状电极，主要用于体内的药物或基因的传递。电极是一个标准的11.5厘米的小钳子，其尖部为嵌入的不锈钢圆盘状电极，两盘的间隙可调至2厘米，并有一个阳极指示器。活体转基因--钳状电极Tweezertrodes电极有两种规格，45-0118为直径：7mm的电极盘，45-0119为直径：10mm的电极盘当把感兴趣的分子注射到体内后，用电极盘夹住相关组织，然后给出电脉冲，使孔道开始形成，将分子整合到直接与电极盘接触的组织细胞内。Tweezertrodes are reusable, tweezer-style in vivo electrodes fordrug or gene delivery in animals. Tweezertrodes consist of astandard 11.5 cm tweezer that has been modified with stainlesssteel circular or disk electrodes at the tip. The gap between theelectrode disks may be adjusted from under 1 mm to over 2 cm.Tweezertrodes come in two models. Model 520 has an electrode diskdiameter of 7 mm and Model 522 has a diameter of 10 mm.Tweezertrodes may be cleaned with a mild detergent and sterilizedwith ethanol or ethylene oxide. These electrodes are connected to apulse generator with the Model 524 Connection Cable, and arecompatible with most BTX Electroporation SystemApplicationsTweezertrodes may be used for many in vivo applications, includinggene and drug delivery. Following localized or systemic injectionof the molecule of interest, the Tweezertrode electrode disks areused to grasp the tissue of interest. An electroporation pulse isthen given, initiating pore formation and incorporation of themolecule into the cells of the tissue in direct contact with theelectrode disks. Suzuki et al. used Tweezertrodes to deliver aGreen Fluorescent Protein (GFP) gene into rat liver. The authorsdemonstrated successful gene transfer and expression in situ.1,2Saito and Nakatsuji used a similar electrode to introduce plasmidDNA during ex-utero and in-utero electroporation.7 Sato et al. usedthe Tweezertrode with the T820 to introduce EGFP expressing plasmidin mouse testis.8 Tweezertrodes complement BTX Genetrode Models508, 510, Caliper Electrode Models 384 and 384L, 2-Needle ArrayModels 530 and 532 as the newest addition to our in vivo electrodeline. The Tweezertrodes can be used to reproduce applicationsrequiring the caliper electrodes, including gene therapy,transdermal drug delivery and electroporation therapy.3,4,5,6 Technical SpecificationsStandard Capabilities Voltage Range: 0V - 200V (Do not use AC current) Pulse Length Range: 1 μsec - 200 msec Physical Characteristics Model 520 Tweezertrode Length: 11.5 cm Electrode Diameter: 7 mm Electrode Material: Stainless steel Model 522 Tweezertrode Length: 11.5 cm Electrode Diameter: 10 mm Electrode Material: Stainless steel Generator Compatibility ECM T820, 630, 830, 2001 Ordering InformationSystem Model Available Configurations 520 Tweezertrode 520Tweezer Electrode with stainless steel plated 7 mm diameterelectrode disk for in vivo applications 523 Tweezertrode 523Tweezer Electrode with stainless steel plated 10mm diameterelectrode disk for in vivo applications Accessory Model Description 524 Tweezertrode Connection Cable ReferencesSuzuki et al., Direct Gene Transfer into Rat Liver Cells by in vivoElectroporation, FEBS Letters, 425: 436-440 (1998) BTX Electroporation Protocol PR0363 (1998) Zhang et al., In vivo transdermal delivery of large molecules bypressure-mediated electroincorporation and electroporation: a novelmethod for drug and gene delivery, Bioelectrochemistry andBioenergetics, 42: 283-292 (1997) Dev, Giordano and Brown, In vivo delivery of gene to rabbit carotidartery by electroporation, Third U.S. / Japan Symposium on DrugDelivery Systems (Abstr.), 38 (1995) Dev, Killing cancer cells with a combination of pulsed electricfields and chemotherapeutic agents, Cancer Watch, 3: 12-14 (1994)BTX Electroporation Therapy Protocols ECT001-ECT005 (1995) Saito and Nakatsuji, Developmental Biology, 240: 237-246 (2001)Sato et al., Molecular Reproduction and Development, 61: 49-56(2002)

BTX 活体专业电极， 专为您的活体电穿孔实验精心设计。BTX为您的活体实验提供了种类繁多，不同尺寸的专业电极，为您的活体电穿孔实验提供最大便利的同时，让您获得最高的转染效率。

人EAR（EGFR/ALK/ROS1）基因联合检测试剂盒（数字PCR法）用于体外定性检测非小细胞肺癌（NSCLC）患者组织样本中EGFR基因突变、ALK基因融合和ROS1基因融合。其中EGFR基因突变可检测19外显子缺失突变（Ex19Del、共检测29种基因型）、21外显子L858R突变及20外显子T790M突变、18外显子G719X突变（3种突变型）、20外显子插入突变（5种基因型）、S768I突变、21外显子L861Q突变、20外显子C797S突变（2种突变型）。ALK基因融合和ROS1基因融合检测采用融合诱导的不平衡转录法（Fusion-induced asymmetric transcription assay， FIATA），根据目标基因的5’端和3’端转录本数量差异，来确定目标基因是否发生融合。该方法不受融合伙伴和融合位点影响，既可检测已知融合，又可检测未知融合，有效避免假阴性结果。灵敏度高，准确性好，结果客观，实验操作简单快速。试剂盒采用新羿生物自主专利的微液滴数字PCR技术，具有准确、简便、快速、特异性高等优点，灵敏度高达0.1%。本试剂盒参照了FDA伴随诊断试剂标准，是高品质、严格质控的基因检测产品，可用于筛选适合EGFR酪氨酸激酶抑制剂（TKI）或ALK/ROS1抑制剂进行治疗的NSCLC患者，同时可辅助对肺癌患者进行高灵敏度早期复发监测，及用药期间疗效与耐药监测。订购信息产品名称目录号规格 人EGFR 41位点突变检测试剂盒1221724测试 人ALK融合基因检测试剂盒1225324测试 人ROS1基因融合检测试剂盒1225524测试