在细胞水平上用共聚焦显微PIV方法研究高压积红细胞流动的近壁不稳定性
In hemodynamics, the inherent intermittency of two-phase cellular-level flow has received little attention.Unsteadiness is reported and quantified for the first-time in the literature using a combination offluorescent dye labelling, time-resolved scanning confocal microscopy, and micro-particle imagevelocimetry (μ PIV). The near-wall red blood cell (RBC) motion of physiologic high-hematocrit blood ina rectangular microchannel was investigated under pressure driven flow. Intermittent flow was associatedwith (1) the stretching of RBCs as they passed through RBC clusters with twisting motions (2) externalflow through local obstacles and (3) transitionary rouleaux formations. Velocity profiles are presentedfor these cases. Unsteady flow clustered in local regions. Extra-cellular fluid flow generated byindividual RBCs was examined using submicron fluorescent microspheres. The capabilities of confocalμ PIV post-processing were verified using synthetic raw PIV data for validation. Cellular interactions andoscillating velocity profiles are presented and 3D data are made available for computational modelvalidation.