3D单分子荧光成像系统 abbelight SAFe360是一款基于单分子定位的显微成像(SMLM)的3D单分子成像系统,它有的DAISY技术整合了散光技术和超临界角光技术,能够大的提高定位精度,达到高的xyz同时15nm的定位精度,可以提供清晰度高的三维的亚细胞结构图像,高同时四色成像观测,可以实时研究不同的结构功能蛋白的共定位信息,在单分子水平研究分子动力学反应以及细胞间的相互作用等。3D单分子荧光成像系统-SAFe 360 设备参数 + 成像模式:PALM、STORM、PAINT、smFRET 、SPT+ 光源模式:Epi、TIRF、HILO+ 高分辨率:15 nm的XYZ轴分辨率+ 超大视野:200 × 200 μm2的视野+ 一次可同时采集1.2 μm深度图像信息+ 高图像深度:10 μm+ 实时漂移矫正+ 高四色同时成像+ 活细胞成像模式 加装TIRFPALMSTORMSPTsmFRET...... 兼容ConfocalSpinning-DeskWidefieldSIMSTED Now We See...... 3D线粒体结构核孔复合物老鼠海马神经元微管蛋白网络 配套试剂 Smart kitCompatible dyes• 10 doses per box• 200 μL per dose• 30 sec prepartion• 2 months in a fridge• 2 weeks on sample• Atto 488, WGA-AF488• AF532, CF532, Cy3b• AF555, AF594, CF555, AF568, CF568, Cy5, MemBriteTM 568, TMR• AF647, CF647, AF680, CF680, MemBriteTM 640, Actin-stain 670, SiR647 发表文献列表[1] Radhakrishnan, A. V., et al. "Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration." The Integrin Interactome. Humana, New York, NY, (2021). 85-113.[2] Jouchet, Pierre, et al. "Nanometric axial localization of single fluorescent molecules with modulated excitation." Nature Photonics (2021): 1-8.[3] Pernier, Julien, et al. "Myosin 1b flattens and prunes branched actin filaments." Journal of cell science 133.18 (2020).[4] Jimenez, Angélique, Karoline Friedl, and Christophe Leterrier. "About samples, giving examples: optimized single molecule localization microscopy." Methods 174 (2020): 100-114.[5] Mau, Adrien, et al. "Fast scanned widefield scheme provides tunable and uniform illumination for optimized SMLM on large fields of view." bioRxiv (2020).[6] Orre, Thomas, et al. "Molecular motion and tridimensional nanoscale localization of kindlin control integrin activation in focal adhesions." bioRxiv (2020).[7] Cabriel, Clément, et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature communications 10.1 (2019): 1980.[8] Woodhams, Stephen G., et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain 160.11 (2019): 2641-2650.[9] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).[10] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.[11] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082. [12] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.[13] Culley, Sian, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.[14] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.[15] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177. [16] Bouissou, Ana?s, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040. [17] Sellés, Julien, et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports 7.1 (2017): 14732.[18] Bourg, Nicolas, et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics 9.9 (2015): 587. 用户单位
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