地磅测感仪

配套CEM赶酸仪消解管实验室有CEM微波消解仪，也有配套的赶酸仪，可是目前需要做一些简单的预处理或者是常规消解，重新买套设备既浪费也占空间，原本的CEM的微波管一只好几千，怕做常规的消解影响微波管的使用，怎么呢？南京瑞尼克为您推出一款消解管，四氟材质尺寸和CEM微波管尺寸一致，放在原本的赶酸仪里面，让赶酸仪既能做消解仪，也能赶酸使用，设计上我们有密封压力消解的，也有敞口常压下消解，让您有多种选择，若需要一些其他设计可以定做。

1+2单功能火灾探测器加温器(ABS-W03) 本产品为不锈钢设计，牢固耐用，加温试验时只需将本试验器充满电和补足丁烷气体，就可加温试验，无需交流电源，机动能力强。●技术性能电源电压：DC7.4V、2200MA/H；加温电路连续工作时间：＞8小时；加温试验数量：＞800只；枪杆长度：1－2.5米（可根据需要加长）；连接杆长度：0.5米输出温度：＞75℃；温源：气体燃烧；气源：丁烷气体。充电时间：8小时；适用适用温度：-5-40℃；外形尺寸：560*190*100净重：1.3公斤。●组成枪头、弯头、连接杆、电池杆、充电器、丁烷气体，简易试验笔、手报吸盘。GA 1157-2014全套消防设施维护保养检测设备2014年5月国家消防法新规范出台后，全国范围内开展实行GA 1157-2014(国标) 一、二、三级全套消防设施维护保养检测设备，对此次执行标准，北京朋利驰科技有限公司，针对此次标准进行设备配置调整，本次调整后完全符合国家GA 1157-2014(国标) 一、二、三级全套消防设施维护保养检测设备的设备配套标准，北京朋利驰科技有限公司在全国范围内都有检测设备的销售，本产品百分百通过本地消防局及技术质监局的通过，如果有不能通过的，北京朋利驰科技有限公司郑重承诺，质保一年，终身维护，免费提供不能检测通过的设备更换，直至检测通过为准。北京朋利驰科技有限公司是致力于生产和代理国际，国内先进的检测仪器仪表和设备诊断工具的高新技术企业。拥有多项自主研发设备产品：感烟探测器功能试验器(HL-08A)，感温探测器功能试验器（HL-3.5）,消火栓测压接头(HL-3),喷水末端试水接头(HL-MD),单功能电子不锈钢感烟探测器功能试验器（KW-JY03）,4+2多功能火灾探测器(AH12),细水雾末端试验装置(HL-S06),多功能消防试水装置，HL-9垂直度检测仪，TDS-100H超声波流量计，各种消防检测箱等。主要经营消防检测设备，主要有红外热像仪，感烟探测器试验装置；感温探测器试验装置；水喷淋系统试水检测装置；消火栓系统试水检测装置；数字兆欧表；数字噪音计；红外测温仪；数字微压计；数字测距仪；垂直度测定仪；多功能工程坡度测定仪；四合一测量仪（温度、湿度、风速、照度） 钳形电流表；数字万用表；数字秒表；附件：数字试电笔、钢卷尺、打火机、加热笔附件盒、发烟棒盒、烟嘴、充电器、丁烷气瓶、仪表测试笔、温度测试探头、多功能刀、测压管、操作手册、铝塑携带箱秒表，数字照度计，数字声级计，数字测距仪，卷尺，数字风速计，数字微压计，消火栓测压接头，超声波流量计，防火涂料测厚仪，喷水末端试水接头，点型感烟探测器功能试验器，点型感温探测器功能试验器，线性光束感烟探测器滤光片，火焰探测器功能试验器，漏电电流检测表，红外测温仪，红外热像仪（选配），便携式可燃气体检测仪，防爆静电电压表，接地电阻测量仪，绝缘电阻测量仪，数字万用表，钳形电流表，泡沫称重电子称，便携式气相色谱仪、便携式红外光谱仪、易燃液体探测仪、便携式可燃气体检测仪、可燃气体探测仪、可燃气体检测管、采样器、薄层色谱分析装置、炭化深度测定仪、金属硬度检验仪、回弹仪、数字温度计、现场勘查灯、碘钨灯、电源线盘、特斯拉计、金属探测器、万用表、接地电阻测量仪、绝缘电阻测试仪、静电电压表、便携式金相显微镜、体视显微镜、小型X光检测仪、照相机、照相冲洗设备、数码照相机、数码摄像机、现场勘查工具箱、数字测距仪、望远镜、尸体袋、物证保存袋、火灾现场勘查专用车、火灾勘查仪器箱、火灾现场勘查工具箱，有毒气体探测仪、军事毒剂侦检仪、可燃气体检测仪、水质分析仪、电子气象仪、音频生命探测仪、视频生命探测仪、雷达生命探测仪、红外热像仪、漏电探测仪、核放射探测仪、电子酸碱测试仪、红外测温仪、移动式生物快速侦检仪、激光测距仪、烟雾视像仪。建筑消防设施检测箱、消防电气检测箱、消防工程检测箱、火灾探测器功能试验箱、火灾报警系统功能检测箱，自动喷水系统、消火栓系统功能检测箱，防排烟系统功能检测箱，火灾勘查仪器箱、火灾现场勘查工具箱、火灾原因调查器材箱、电器性能检查箱、消防监督检查箱、消防工程验收箱、消防监督技术装备箱、消防监督器材包装箱、消防检查辅助器材箱、消防电气性能检查箱、消防监督检查验收箱、消防监督技术装备产品、消防产品现场检测箱、消防产品现场检查判定箱、防火门破拆工具箱、防火涂料检测箱、常规新产品检测箱、专业测量仪器箱、地铁消防检测箱、防火检查仪器箱、派出所消防监督装备、机场、油田、军事设施、铁路地铁、港口码头、学校消防检查设备、智能建筑与安防工程检测仪器序号设备名称一级标配二级标配备注1秒表43量程不小于15min；精度：0.1s2卷尺43量程不小于30m；精度：1mm3游标卡尺43量程不小于150m；精度：0.02mm4钢直尺43量程不小于50cm；精度：1mm5直角尺43主要用于对消防软管卷盘的检查6电子秤11量程不小于30kg7测力计21量程：50N～500N；精度：±0.5%8强光手电64充电式，LED冷光源9激光测距仪43量程不小于50m；精度：3mm10数字照度计43量程不小于2000lx；精度：±5%11数字声级计43量程：30dB～130dB；精度：1.5dB12数字风速计43量程：0m/s～45m/s；精度：±3%13数字微压计21量程：0Pa～3000Pa；精度：±3%；具有清零功能，并配有检测软管14数字温湿度计21用于环境温湿度检测15超声波流量计21测量管径范围：15mm～700mm；精度：±1%16数字坡度仪11量程：0-±90o；精度：±0.1o17垂直度测定仪21量程：0mm～500mm；精度：±0.2μm18消火栓测压接头43压力表量程：0MPa～1.6MPa；精度：1.6级19喷水末端试水接头43压力表量程：0MPa～0.6MPa；精度：1.6级20防爆静电电压表1-量程：0kV～30kV；精度：±10%21接地电阻测量仪32量程：0Ω～1000Ω；精度：±2%22绝缘电阻测量仪32量程：1MΩ～2000 MΩ；精度：±2%23数字万用表43可测量交直流电压、电流、电阻、电容等24感烟探测器功能试验器43检测杆高度不小于2.5ｍ，加配聚烟罩，内置电源线；连续工作时间不低于２h25感温探测器功能试验器43检测杆高度不小于2.5ｍ，内置电源线；连续工作时间不低于２h26线型光束感烟探测器滤光片11减光值分别为0.4dB和10.0dB各一片；具备手持功能27火焰探测器功能试验器11红外线波长大于等于850nm，紫外线波长小于等于280nm。检测杆高度不小于2.5ｍ28漏电电流检测仪21量程：0A～2A；精度：0.1mA29超声波泄漏检测仪1-用于可燃气体、液体泄漏检测30便携式可燃气体检测仪21可检测一氧化碳、氢气、氨气、液化石油气、甲烷等可燃气体浓度，并发出声光报警。31数字压力表21量程：0MPa～20MPa；精度：0.4级；具有清零功能32细水雾末端试水装置21压力表量程：0MPa～20MPa；精度：0.4级秒表HL-3消火栓测压接头：压力表量程：0MPa～1.6MPa；精度：1.6级细水雾末端试水装置超声波流量计线型光束感烟探测器滤光片数字微压计火焰探测器功能试验器卷尺电子称HL-4消火栓测压接头压力表量程：0MPa～1.6MPa；精度：1.6级HL-MD喷水末端试水接头:压力表量程：0MPa～0.6MPa；精度：1.6级HL-XM30消防试水检测箱（消火栓测压接头HL-3，喷水末端试水接头HL-MD，30米卷尺）感烟感温探测器功能试验器二合一AH-YW19(里面一个烟一个温)：检测杆高度不小于2.5ｍ，加配聚烟罩，内置电源线；连续工作时间不低于２hSD-T6-2B强光手电：充电式，LED冷光源超声波泄露检测仪HL-GA1157消防常用仪器箱（北京朋利驰科技有限公司配置：消防不锈钢防水秒表，数字声级计，数字风速仪，数字照度计，激光测距仪，数字微压计，数字温湿度计，数字万用表，绝缘阻测试仪，接电阻测试仪，压力测试管，检测笔）

北京核地FD216-FD-216测氡仪土壤采样杆， 说明书，操作指南，售后服务，特点1.体积小、重量轻，便于携带。2.灵敏度高、功耗低，交、直流两用，直流电源可支持仪器工作30h。3.USB数据传输接口，蓝牙打印。北京核地FD216-FD-216测氡仪土壤采样杆，北京核地FD216-FD-216测氡仪土壤采样杆，技术性能1.灵敏度：≥0.68cpm/[Bq?m-3]2.本底计数率：≤0.3cpm3.测量范围环境空气氡：（3～100000）Bq/m3土壤氡：（300～300000）Bq/m3氡析出率：（0.001～10.000）Bq/[m2?s]水中氡：（0.003～100）Bq/L4.测量重复性误差：≤5%（氡室浓度2000Bq/m3，环境湿度65%，温度25℃）。5.长期稳定性（8h）误差:≤10%6.电源：锂离子充电电池/交流电，电池供电可连续工作30h。7.工作环境温度：-10℃～+40℃湿度：相对湿度≤90%（+40℃）8.探测器：硫化锌ZnS(Ag)和光电倍增管组合系统9.数据存储：可存储2000个数据10.操作模式：单点检测或连续监测11.显示器：LCD液晶显示12.取气方式：主动泵吸式13.测量时间空气氡：31min土壤氡：11min氡析出率：30min水中氡：31min14.打印数据：日期、时间、点号和检测结果15.尺寸：（330×210×170）mm16.重量：5㎏（主机）北京核地FD216-FD-216测氡仪土壤采样杆，北京核地FD216-FD-216测氡仪土壤采样杆，说明书，操作指南，售后服务，特点，认证中国计量科学研究院检定并出具检定证书

感烟探测器拆装器，感温探测器拆装器，探测器折装工具，消防专用拆卸工具，感烟探测器功能试验器拆卸工具烟温探测器拆装器专门用来海湾消防烟温感二次拆装，特别适宜先装设备底座及大面积拆装、或探测器下方有障碍物——诸如，楼梯坡道、桌椅、床铺、吊灯上方等及特别高的场所，可规避高空作业安全，节省了登高设施使用费及大量的人工准备工时费。实为不可多得的划时代安装工具。此产品出现可颠覆现有施工安装程序，可在穿线完毕的同时先安装接线固定底座，最后在测量线路无故障后再使用拆装器安装设备，施工效率高，且测试线路绝缘时不用考虑已经安装部分设备而有所顾忌。 高压配电室、配电机柜上方、内燃机组上方、变压器上等特殊无法触及到的场合，以及高架仓库、高空超高位置安装的探测器，出现误报后无法更换，造成整个火灾报警系统运行不稳定。

赶酸器GS赶酸器:又称赶酸电热板、赶酸板、样品处理器、赶酸仪、消解器。适用于食品、疾控、农科院、医药、农业、林业、环保、化工、生化等行业以及高等院校、科研部门对微波消解、高压消解的后期赶酸,是原子吸收、原子荧光、ICP- AES、AA、ICP- MS等分析仪器的理想配套产品。技术参数型号GS型孔数、孔径要求可根据客户样品量加工成12 、16、20、24、36、42、54、63、72等位数及特殊孔径×深度的电热板加热方式环绕式电加热 PID数显温控范围室温-200℃控温精度±1℃加热板块表面防腐进口Teflon涂层加热板材质铝合金额定电压220V连续工作时间＞48h配套产品美国CEM、迈尔斯通、利曼、上海新仪、北分瑞利、安东帕、上海屹尧等厂家的内罐，消解罐内杯、烧杯、试管、坩埚、消解罐内杯、PFA溶样罐等产品特点：1、进口优质PFA 涂层, 一抹即净、永不生锈,特氟隆防腐铸铝加热板升温速度快2、工作温度：室温至260℃，可连续工作48h,5-30分钟可升到200℃（因孔深而异）3、一次性处理样品量大，用时短，与只是靠平面供热辐射立面的一般孔式消解仪相比，能够提高50%的工作效率4、环绕式加热，孔间温差小，加热均匀，使用方便，寿命长。5、板面与控制盒可以做成分体式，裸露部分都采用PFA管子包裹，这样在实验过程中的酸雾就不会腐蚀控制盒里的元器件，从而增加了使用寿命，多个样品同时处理无交叉污染6、消解均匀：器皿底部及360°包裹式加热，使得样品加热无盲点，稳定性好，均匀性很好8、仪器周围可增置外壳，美观且保温用途：专为实验室设计的，是样品加热消解、蒸酸、煮沸、赶酸的好帮手，也是原子吸收，原子荧光等分析仪器的理想配套产品。可以检测食品、药品、乳制品等等中铅、镉、铬、汞、砷、锌、硒、锰等重金属元素，以及微量元素；测定土壤中重金属和微量元素的消解等。

北京核地FD-216测氡仪土壤采样杆套件北京核地FD216环境氡、土壤氡测量仪，说明书，操作指南，售后服务，特点，技术性能1.灵敏度：≥0.68cpm/[Bq?m-3]2.本底计数率：≤0.3cpm3.测量范围环境空气氡：（3～100000）Bq/m3土壤氡：（300～300000）Bq/m3氡析出率：（0.001～10.000）Bq/[m2?s]水中氡：（0.003～100）Bq/L4.测量重复性误差：≤5%（氡室浓度2000Bq/m3，环境湿度65%，温度25℃）。5.长期稳定性（8h）误差:≤10%6.电源：锂离子充电电池/交流电，电池供电可连续工作30h。7.工作环境温度：-10℃～+40℃湿度：相对湿度≤90%（+40℃）8.探测器：硫化锌ZnS(Ag)和光电倍增管组合系统9.数据存储：可存储2000个数据10.操作模式：单点检测或连续监测11.显示器：LCD液晶显示12.取气方式：主动泵吸式13.测量时间空气氡：31min土壤氡：11min氡析出率：30min水中氡：31min14.打印数据：日期、时间、点号和检测结果15.尺寸：（330×210×170）mm16.重量：5㎏（主机）北京核地FD216环境氡、土壤氡测量仪， 说明书，操作指南，售后服务，特点，认证中国计量科学研究院检定并出具检定证书

波长敏感探测器(WS)筱晓光子供应波长敏感探测器(WS)，利用特定波长在硅基材料的辐射吸收深度来实现。硅晶体两个相互垂直的p-n结的结构特征，特别适合于单色光的波长检测。Wavelength sensitive diodes (particularly suitable for monochromatic light)Type No.Dark currentRise time Diode 1Rise time Diode 2ChipPackage5V0V 1kΩ0V 1kΩnAnsnsWS7.56(Discontinued)PCBA10100001000WS7.56TO510100001000WS7.56TO5i5100001000

石墨赶酸器、石墨赶酸电热板一、产品介绍石墨赶酸器又称赶酸板、样品处理器、赶酸仪、消解器。适用于食品、疾控、农科院、医药、农业、林业、环保、化工、生化等行业以及高等院校、科研部门对微波消解、高压消解的后期赶酸,是原子吸收、原子荧光、ICP- AES、AA、ICP- MS等分析仪器的理想配套产品。石墨赶酸器参数型 号ZH控温范围室温-260℃控温精度±0.1℃，高温度差3-5℃加热材质优质石墨+特氟龙防腐涂层样品孔数20、25、30、40、42、48孔等（可根据客户要求加工定制）控显方式PID控温数显电 源220v/50Hz优点1、孔间温差±1-1.5℃，板面低温度和高温度＜3-5℃2、保证了消解、赶酸的均一性配套器皿消解管（31*100mm）、55ml微波罐（29*160-170mm），其他品牌微波，消解罐内杯，烧杯、坩埚等我司石墨消解器的优点优点1：耐高温耐酸碱及有机溶剂腐蚀，稳定性好；优点2：消解样品速度快，可批量处理样品；优点3：全封闭设计，很大程度防止热量散失的同时，还可有效避免避免酸雾入侵，对设备元件造成损害；优点4：清洁耐腐蚀：采用特别的喷涂工艺，涂层均匀、牢固、长期使用也不会脱落；优点5：采用先进的一体环绕加热方式，样品各部位受热均匀，消解快速；优点6：操作方便，环保节能，节省经济成本；优点7：PID温控系统，性能优良，控温精度高，可达±1℃。优点8：可调节加热速率，实现程序升温并控制加热保持时间，升温速率稳定；仪器的维护1、仪器主机：定期对仪器的电源线及电源线所接电源如插排、墙插等进行检查，如有损坏 老化及时更换。2、清理：将仪器工作面擦拭干净，上面不要有水滴，污物等3、安全：样品处理需要用到各种不同的酸，如HCl、HNO₃、HF等。大部分酸具有挥发性而且对人体不好，因此建议在通风的环境下使用仪器，并做好各种防护措施。4、使用：在说明书指定的工作环境下使用，不用时要拔掉电源，清洁石墨块表面污垢时，要用布轻擦，尽量不使用有机溶剂，不能损坏表面的涂层。5、存储：消解或者加热后，不再用到消解管时应该对其进行清理、并可以到下次实验再次使用，可以节省成本

赶酸器GS赶酸器:又称赶酸电热板、赶酸板、样品处理器、赶酸仪、消解器。适用于食品、疾控、农科院、医药、农业、林业、环保、化工、生化等行业以及高等院校、科研部门对微波消解、高压消解的后期赶酸,是原子吸收、原子荧光、ICP- AES、AA、ICP- MS等分析仪器的理想配套产品。技术参数型号GS型孔数、孔径要求可根据客户样品量加工成12 、16、20、24、36、42、54、63、72等位数及特殊孔径×深度的电热板加热方式环绕式电加热 PID数显温控范围室温-200℃控温精度±1℃加热板块表面防腐进口Teflon涂层加热板材质铝合金额定电压220V连续工作时间＞48h配套产品配套美国CEM、配套迈尔斯通、配套利曼、配套上海新仪、配套北分瑞利、配套安东帕、配套上海屹尧等厂家的内罐，配套消解罐内杯、烧杯、试管、坩埚、消解罐内杯、PFA溶样罐等

OA-360智能石墨赶酸仪被广泛应用于实验室样品消解的赶酸处理，采用包裹式加热，具有可设置多段温度赶酸的智能程序功能，支持孔数、孔径及孔深定制，实现高效赶酸处理。可作为各种微波消解仪配套赶酸设备，解决微波消解赶酸难的问题。产品特点1、多孔设计，赶酸更快批量处理能力强，大大缩短赶酸时间；标配36位，可根据消解罐大小定制。2、高纯石墨块包裹式加热高纯石墨体具有良好的导热性，加热均匀，平行性好。立体包裹式加热，热量损失更少，效率更高。3、远程控制，程序升温采用PID程序温控系统，控温精度±0.2℃；实现多段升温、保持时间、升温时间，自动停止加热。4、工步指示灯程序工步显示灯，指示当前实验样品消解步骤，状态一目了然。5、高品质工艺，耐腐蚀石墨体及整机壳体喷涂特氟龙涂层，易清洁、耐腐蚀；整机外围无金属部件，可在强酸强碱等恶劣环境中放心使用。6、真实的样品温度石墨赶酸仪可选配外置温度探针，保证了样品的真实温度，使样品赶酸更加到位。应用领域环境监测：污水、饮用水、淤泥、矿泥、排污、土壤等农业食品检验：大米、奶粉、鱼类、蔬菜、烟草、植物、化肥等产品质量控制：化妆品、副食品、工业制品等科学研究：实验分析、项目开发等疾病预防控制：生物样品、人体毛发等

用途299/300型摆杆硬度试验仪用于作摆杆阻尼试验，评价清漆、色漆和相关涂料的机械阻尼 特性。测试原理置于被测表面上的标准摆杆的摆动跟被测面的&ldquo 柔软度&rdquo 是成比例的。结构和功能标准器具由托架和带调节水平螺栓的底盘组成。托架上装有一样板架可放样板250 100 15mm。仪器有一丙烯酸玻璃罩防尘防气流，样板位有一开口。摆杆硬度试验仪用罩外的控制操作。可选附件有两种带支点架，不锈钢制摆杆。 摆杆：按科尼格法，依DIN 53 157标准。 摆杆：按珀萨兹法，依NFT 30-016标准。摆杆可自动调整，避免了费时的调校光电感应器。有两种测量模块 基本型用一个机械杠杆让摆杆偏移，松开钢缆摆杆开始摆动。 自动型摆杆的偏移和释放自动完成。自动型有下列优点： 使用方便 结果稳定 摆杆换型简单使用任何一款测量模块，都须在控制器上输入所用摆杆。从4位液晶显示器上可交替读到 摆动次数 测试时间，或 摆动周期技术数据基本仪表外形尺寸： 高： 700 mm宽： 325 mm深： 345 mm净重： 约 17 kg测量组件 (基本型)外形尺寸： 高：60mm宽：235mm深：70mm净重： 约0.5kg测量组件 (自动型)外形尺寸： 高：65mm宽：235mm深：107mm净重:： 约0.75kg控制器外形尺寸： 高：35mm宽：167mm深：67mm净重： 约0.4kg电压： 230V AC  10%功耗： 25VA

产品名称：草甘膦检测专用色谱柱 品牌：莱博瑞特 产品型号： LABORAT LET-SAX(阴离子)（5um*250*4.6mm） 应用领域：食品、药品、环境、卫生、制药、科研院所 产品简介： 色谱柱作为色谱系统的心脏，色谱柱的优劣直接影响分离效果。莱博瑞特同知名色谱柱生产商合作，从硅胶原料的选择到键合工艺，从色谱柱的装填到检测,各道工序都经过严格监管、精心设计及科学检验，生产的色谱柱具有柱效高、选择性好、分析速度快等特点，在草甘膦类农药的分析实验中，具有很好的分离效果和能力，深受用户好评。 产品应用案例：生活饮用水中草甘膦检测应用方案1. 参照标准GB/T 5750.9-2006 《生活饮用水标准检验方法 农药指标》 2. 色谱图

北京/上海/广州液相色谱仪配件-柱塞杆南京科捷分析仪器有限公司是专业研究、开发、制造和销售色谱仪（气相色谱仪、液相色谱仪）、比表面积测定仪、高纯气体分析设备、色谱配件、色谱试剂以及其他分析仪器的高科技企业。以下是各柱塞杆详细介绍：安捷伦系列柱塞杆：产品名称规格型号相关配置产地柱塞杆Agilent1050/11005063-6589安捷伦柱塞杆Agilent10505062-244安捷伦柱塞杆Agilent1050/11005062-8516安捷伦岛津系列的柱塞杆：（1）LC-10ADvp柱塞杆：228-35601-91（相关配置）（2）LC-10ATvp柱塞杆：228-35009-92TSP的柱塞杆：SP8800/8810柱塞杆:A3102-010北京/上海/广州液相色谱仪配件-柱塞杆南京科捷是专业研究、开发、制造和销售色谱仪（气相色谱仪、液相色谱仪）、高纯气体分析设备、色谱配件（气相进样针,微量注射器,毛细管柱,填充色谱柱,色谱柱,进样垫,气体三通阀,四通阀,六通阀,八通阀,十通阀,十二通阀,检测器灯,检测器灯,自动进样器,泵零件,检测灯,自动进样器,泵零件,检测灯,泵零件,检测灯, 自动进样器,泵零件,保护柱套,管路配件,国产液相色谱柱,进口液相色谱柱,SPE固相萃取柱,过滤膜,液相进样针,阀配件,柱塞杆,密封圈,氘灯,高效色谱级试剂）、色谱试剂、顶空,顶空进样器以及其他分析仪器的高科技企业。

干胶仪配件是干燥凝胶的凝胶干燥仪,采用微处理器控制温度，提供室温+5～90℃的温度范围，干胶仪配件需要连接真空泵，从底板均匀去除凝胶湿气，均匀干燥凝胶。干胶仪配件特点周围采用沟槽设计采用微处理器控制温度双LED屏显示采用优质硅胶填充以确保连接真空泵时具有良好气密性凝胶从底部加热，真空也从凝胶下边驱走湿气使用硅橡胶盖密封，具有绝佳的密封性干胶仪配件参数 温度分辨率：0.1℃温度标定：是温度范围：室温+5～90℃温度均匀度：+/-0.2℃干燥面积： 21x31cm，或 35x45cm计时器：0-999min工作温度：室温-40℃孚光精仪是全球领先的进口科学仪器和实验室仪器领导品牌服务商，产品技术和性能保持全球领先,拥有包括凝胶成像仪在内的全球最为齐全的实验室和科学仪器品类，世界一流的生产工厂和极为苛刻严谨的质量控制体系，确保每个一产品是用户满意的完美产品。我们海外工厂拥有超过3000种仪器的大型现代化仓库，可在下单后12小时内从国外直接空运发货，我们位于天津保税区的进口公司众邦企业（天津)国际贸易公司为客户提供全球零延误的进口通关服务。更多关于干胶仪价格表,干胶仪品牌等诸多信息，孚光精仪会在第一时间更新并呈现出来，了解更多内容请关注孚光精仪官方网站方便获取！

线型光束感烟探测器滤光片减光片减光片：减光值为0.9db和10db。检查方法：确认线型光束感烟火灾探测器与火灾报警控制器连接并接通电源，处于正常监视状态。将减光值为0.9dB的滤光片置于线型光束感烟火灾探测器的光路中并尽可能靠近接收器，观察火灾报警控制器的显示状态和线型光束感烟火灾探测器的报警确认灯状态。如果30s内发出火灾报警信号，记录其响应阈值小于1.0dB，结束试验。如果30s内未发出火灾报警信号，继续试验，将减光值为10dB的滤光片置于线型光束感烟火灾探测器的光路中并尽可能靠近接收器，观察火灾报警控制器的显示状态和线型光束感烟火灾探测器的报警确认灯状态。如果30s内未发出火灾报警信号，记录其响应阈值大于10.0dB。判断标准：探测器的响应阈值应不小于1.0db，不大于10db. 规格单位厂价25mL支1200.0050mL支1600.00100mL支2000.00

石墨赶酸器、石墨赶酸电热板一、产品介绍石墨赶酸器又称赶酸板、样品处理器、赶酸仪、消解器。适用于食品、疾控、农科院、医药、农业、林业、环保、化工、生化等行业以及高等院校、科研部门对微波消解、高压消解的后期赶酸,是原子吸收、原子荧光、ICP- AES、AA、ICP- MS等分析仪器的理想配套产品。石墨赶酸器参数型 号ZH控温范围室温-260℃控温精度±0.1℃，最高温度差3-5℃加热材质优质石墨+特氟龙防腐涂层样品孔数20、25、30、40、42、48孔等（可根据客户要求加工定制）控显方式PID控温数显电 源220v/50Hz优点1、孔间温差±1-1.5℃，板面低温度和高温度＜3-5℃2、保证了消解、赶酸的均一性配套器皿消解管（31*100mm）、55ml微波罐（29*160-170mm），其他品牌微波，消解罐内杯，烧杯、坩埚等我司石墨消解器的优点优点1：耐高温耐酸碱及有机溶剂腐蚀，稳定性好；优点2：消解样品速度快，可批量处理样品；优点3：全封闭设计，很大程度防止热量散失的同时，还可有效避免避免酸雾入侵，对设备元件造成损害；优点4：清洁耐腐蚀：采用特别的喷涂工艺，涂层均匀、牢固、长期使用也不会脱落；优点5：采用先进的一体环绕加热方式，样品各部位受热均匀，消解快速； 优点6：操作方便，环保节能，节省经济成本； 优点7：PID温控系统，性能优良，控温精度高，可达±1℃。优点8：可调节加热速率，实现程序升温并控制加热保持时间，升温速率稳定；仪器的维护1、仪器主机：定期对仪器的电源线及电源线所接电源如插排、墙插等进行检查，如有损坏 老化及时更换。2、清理：将仪器工作面擦拭干净，上面不要有水滴，污物等3、安全：样品处理需要用到各种不同的酸，如盐酸、HNO₃ 、HF等。大部分酸具有挥发性而且对人体不好，因此建议在通风的环境下使用仪器，并做好各种防护措施。4、使用：在说明书指定的工作环境下使用，不用时要拔掉电源，清洁石墨块表面污垢时，要用布轻擦，尽量不使用有机溶剂，不能损坏表面的涂层。5、存储：消解或者加热后，不再用到消解管时应该对其进行清理、并可以到下次实验再次使用，可以节省成本。安全使用及注意事项1、 使用含HClO4的消解剂时，控制好HClO4量和消解温度等条件，安全才有保障，否则有燃烧、爆炸的危险。2、 在操作仪器时出现安全问题，立刻关闭仪器，禁止继续操作。3、 由于加热H₂ SO₄ 、盐酸等消解液及样品，消化管温度比较高，小心灼伤。4、 在操作仪器的时候，要穿着防护设备和佩带护眼罩。5、 仪器出现故障时，及时与厂家联系。

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。Product Description:bioGenousTMHepatocellular Carcinoma Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human hepatocellular carcinoma organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMHepatocellular Carcinoma Organoid Basal MediumK2105-HCC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMHepatocellular Carcinoma Organoid Supplement B (50x)K2105-HCC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMHepatocellular Carcinoma Organoid Supplement C (250x)K2105-HCC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Hepatocellular Carcinoma Organoid Complete MediumUse a sterile technique to prepare the hepatocellular carcinoma organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Hepatocellular Carcinoma Organoid Supplement B(50x) and Hepatocellular Carcinoma Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Hepatocellular Carcinoma Organoid Supplement B(50x) and 40μL Hepatocellular Carcinoma Organoid Supplement C(250x) to 9.76mL Hepatocellular Carcinoma Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Hepatocellular Carcinoma Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Hepatocellular Carcinoma OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human hepatocellular carcinoma tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 15 min to 45 min. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of hepatocellular carcinoma organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived hepatocellular carcinoma organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥5 times every 1 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 3 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm hepatocellular carcinoma organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

适用于CE/Thermo Finnigan 1108/1110/2500仪器，Costech ECS4010Thermo EA 、SerCon货号：46820001 用途：用作各种填充、还原、燃烧管

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Cellulose Filter - organics - 10 ml, 100 each | 060941Cellulose Filter - organics - 10 ml, 100 each订货信息：部件号品名描述数量060941Cellulose Filter - organics - 10 ml, 100 eachCellulose Filter - organics - 10 ml, 100 eachEA

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Traceable长杆温度计产品特点:开/关开关提供更长的电池寿命，°F/°C可切换范围长杆允许在搅拌时读取读数，非常适合在培养箱和烤箱中使用包括：电池、保护套、Traceable证书货号量程分辨率精度杆长长度90225-15 ‒ 50 to 150°C0.1° from ‒ 20 to 200°(1° otherwise)±1°C8 inches11 inches90205-01‒ 50 to 150°C0.1° from ‒ 20 to 200°(1° otherwise)±0.2°C at tested points8 inches11 inches94460-40‒ 50 to 300°C0.1° from ‒ 20 to 200°(1° otherwise)±1°C11%-inches14%-inches90205-02‒ 50 to 300°C0.1° from ‒ 20 to 200°(1° otherwise)±0.5°C at tested points11%-inches14%-inches

序号产品名称型号参数数量1（金属）秒表KW-15A不锈钢量程不小于15min 精度0.1s(32卷尺30m量程：30m 精度：1mm2卷尺长城5m量程：5m 精度：1mm23游标卡尺广陆150mm量程：150mm 精度：0.02mm34钢直尺长城50cm量程：50cm 精度：1mm35直角尺加厚30cm主要用于对消防软管卷盘的检测查36（台称）电子称30kg量程不小于30kg，计重称17（数显）测力计HL-500N数显型量程：50N-500N 精度：±0.5%18强光手电SD-T6-2B警用充电式，LED冷光源49激光测距仪KW-60T量程不小于50m 精度：3mm310数字照度计HL-1550量程不小于2000lx 精度：±5%311数字声级计GM1357量程：30dB～130dB 精度1.5db312数字风速计KW-45S量程：0m/s～45m/s 精度：±3%313数字微压计DP1000-IIIB量程：0Pa～3000pa 精度：±1% 具有清零功能，并配有检测软管114数字温湿度计HTC-1用于环境温湿度检测115超声波流量计TDS-100H测量管径范围：0mm～300mm；精度：±1%116数字坡度仪LS160/IP65量程：0°~±90° 精度：±0.1° 118垂直度测定仪HL-9量程：0mm～500mm；精度：±0.2μm118消火栓测压接头HL-4(标准表)压力表量程：0MPa～1.6MPa；精度：1.6级319喷水末端试水接头HL-MD(标准表)压力表量程：0MPa～0.6MPa；精度：1.6级320接地电阻测量仪HL1214A量程：0Ω～1000Ω；精度：±2%221绝缘电阻测量仪HL-1652C量程：1MΩ～2000 MΩ；精度：±2%222数字万用表UT890D可测量交直流电压、电流、电阻、电容等323感烟探测器功能试验器不锈钢KW-JY03检测杆高度不小于2.5ｍ，加配聚烟罩，内置电源线；连续工作时间不低于２h324感温探测器功能试验器不锈钢KW-W03检测杆高度不小于2.5ｍ，内置电源线；连续工作时间不低于２h325线型光束感烟探测器滤光片AH410减光值分别为0.4dB和10.0dB各一片；具备手持功能125火焰探测器功能试验器无明火全不钢AH-ZH28红外线波长大于等于850nm，紫外线波长小于等于280nm。检测杆高度不小于2.5ｍ127漏电电流检测仪KW-1220量程：0Ａ～2Ａ；精度：0.1mＡ128便携式可燃气体检测仪HL-90可检测一氧化碳、氢气、氨气、液化石油气、甲烷等可燃气体浓度129数字压力表HL-20M量程：0MPa～20MPa；精度：0.4级；具有清零功能130细水雾末端试水装置HL-S06压力表量程：0MPa～20MPa；精度：0.4级131消防检测仪器箱PLC-JD盛放消防检测仪器332消防检测仪器箱VC11B盛放消防检测工具3

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMSmall Cell Lung Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human Small Cell Lung Cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMSmall Cell Lung Cancer Organoid Basal MediumK2121-SL-A100/A500100mL/500mL4℃，12 monthsbioGenousTMSmall Cell Lung Cancer Organoid Supplement B (50x)K2121-SL-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMSmall Cell Lung Cancer Organoid Supplement C (250x)K2121-SL-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Small Cell Lung Cancer Organoid Complete MediumUse a sterile technique to prepare the small cell lung cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Small Cell Lung Cancer Organoid Supplement B(50x) and Small Cell Lung Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Small Cell Lung Cancer Organoid Supplement B(50x) and 40μL Small Cell Lung Cancer Organoid Supplement C(250x) to 9.76mL Small Cell Lung Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Small Cell Lung Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Small Cell Lung Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human small cell lung cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of small cell lung cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived small cell lung cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥3 times every 1 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 3 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm small cell lung cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMCervical Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human cervical cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMCervical Cancer Organoid Basal MediumK2169-CC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMCervical Cancer Organoid Supplement B (50x)K2169-CC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMCervical Cancer Organoid Supplement C (250x)K2169-CC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Cervical Cancer Organoid Complete MediumUse a sterile technique to prepare the cervical cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Cervical Cancer Organoid Supplement B(50x) and Cervical Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Cervical Cancer Organoid Supplement B(50x) and 40μL Cervical Cancer Organoid Supplement C(250x) to 9.76mL Cervical Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Cervical Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Cervical Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human cervical cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of cervical cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived cervical cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm cervical cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

用途：用于盛装固体样本的反应管 适用仪器为： CE/Thermo Finnigan 1108/1110/1500(2)/2500Costech: 061104Thermo: 46820060Costech ECS4010Thermo EA1108CHNSOThermo EA1110CHNSOThermo NA1500NCS2Thermo NA2000/2100Thermo NA2500NCS

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMBreast Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human breast cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMBreast Cancer Organoid Basal MediumK2147-BC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMBreast Cancer Organoid Supplement B (50x)K2147-BC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMBreast Cancer Organoid Supplement C (250x)K2147-BC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Breast Cancer Organoid Complete MediumUse a sterile technique to prepare the breast cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Breast Cancer Organoid Supplement B(50x) and Breast Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Breast Cancer Organoid Supplement B(50x) and 40μL Breast Cancer Organoid Supplement C(250x) to 9.76mL Breast Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Breast Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Breast Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human breast cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1.5 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well.CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of breast cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived breast cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm breast cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMOvarian Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human Ovarian Cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMOvarian Cancer Organoid Basal MediumK2168-OC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMOvarian Cancer Organoid Supplement B (50x)K2168-OC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMOvarian Cancer Organoid Supplement C (250x)K2168-OC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Ovarian Cancer Organoid Complete MediumUse a sterile technique to prepare the ovarian cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Ovarian Cancer Organoid Supplement B(50x) and Ovarian Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Ovarian Cancer Organoid Supplement B(50x) and 40μL Ovarian Cancer Organoid Supplement C(250x) to 9.76mL Ovarian Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Ovarian Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Ovarian Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human ovarian cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1.5 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of ovarian cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived ovarian cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm ovarian cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

类器官（Organoids）是指将成体干细胞或多能干细胞在体外三维培养形成的具有一定空间结构的组织类似物。类器官在组织结构、细胞类型、自我更新能力和功能等方面与来源组织高度一致，从而在发育生物学、疾病造模、精准医学、药物研发、基因和细胞疗法、感染和免疫以及再生医学等生物医学的多个领域展现出独特的优势。产品介绍Product Description:bioGenousTMGastric Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human gastric cancer organoid s. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.技术参数Product Information:ComponentComponent Cat#VolumeStorage & StabilitybioGenousTMGastric Cancer Organoid Basal MediumK2179-GC-A100/A500100mL/500mL4℃，12 monthsbioGenousTMGastric Cancer Organoid Supplement B (50x)K2179-GC-B100/B5002mL/10mL-20℃,avoid repeated freeze-thaw cycles, 12 monthsbioGenousTMGastric Cancer Organoid Supplement C (250x)K2179-GC-C100/C5000.4mL/2mL-20℃, avoid repeated freeze-thaw cycles, 12 monthsPreparation of Gastric Cancer Organoid Complete MediumUse a sterile technique to prepare the gastric cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.1.Thaw Gastric Cancer Organoid Supplement B(50x) and Gastric Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.NOTE:Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.2.Add 200μL Gastric Cancer Organoid Supplement B(50x) and 40μL Gastric Cancer Organoid Supplement C(250x) to 9.76mL Gastric Cancer Organoid Basal Medium. Mix thoroughly.NOTE:If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Gastric Cancer Organoid Supplement B contains fungicide and antibiotics (50x).Protocol for Establishment ofPatient-Derived Gastric Cancer OrganoidsCAUTIONStudies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.Establishment of Organoids from Primary Tissue1.Collect primary human gastric cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.2.Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.3.Rinse the tissuewith Cancer Organoid Basal Medium (B213152) or DPBS twice.4.Mince the tissue into small fragments of 1-3 mm3in a cell culture dish using surgical scissors or scalpels.5.Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.6.Add FBS to the tissuedigestionmixture to a final concentration of 2%, and filter using a 100 μm cell strainer.7.Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.8.Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C,repeat this step once more time.9.Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.CRITICAL:Do not overly dilute the Matrigel (Matrigel should be 70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.10.Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30μL each around the center of the well..CRITICAL:Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.11.Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for15-25 minto let the Matrigel solidify.12.Prepare the required amount of gastric cancer organoid complete medium.13.Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.CRITICAL:Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.14.Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO2.15.Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.16.Closely monitor the organoid formation. Ideally, patient-derived gastric cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.Splitting and Passaging of Organoids17.Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.18.Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.19.Centrifuge organoids at 250g for 3 min at room temperature.20.Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time inthe Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL ofCancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.CRITICAL:Do not dissociate in Organoid Dissociation Solution for 5 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.21.After shearing is complete, wash once by topping up with 1 mL ofCancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.22.Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO2for 15–25 min.23.Pre-warm gastric cancer organoid complete medium at 37 °C.24.After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.25.Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO2until the organoids are needed for further experiments.

立体定位仪耳杆EB-1是Narisihige公司SN系列小动物立体定位仪器的配件，立体定位仪耳杆EB-1用于对小动物耳朵立体定位。用于SN系列的耳固定杆EB-1Narisihige的 EB-1 是用于SN系列立体定位仪器的标准耳固定杆（一对）。立体定位仪耳杆EB-1规格长度/重量长115mm, 80g*这是一个NARISHIGE“组2”的特殊订单项目。因为需要根据用户需求进行定制，订货交付时间通常为4周，一旦下单则不可撤销和不可退换。用于SN系列的中空耳固定杆-EB-2A Narisihige 的 EB-2A 是专门用于SN系列仪器的耳固定杆。类型A内部中空，可用于听觉实验。也请考虑以下这些耳固定杆：EB-2B 专用耳固定杆（无断裂型，一对）EB-2C 专用大鼠耳固定杆（一对）EB-2D 专用豚鼠耳固定杆（一对）Specifications规格长度/重量长140mm, 95g (per pair)*这是一个NARISHIGE“组2”的特殊订单项目。因为需要根据用户需求进行定制，订货交付时间通常为4周，一旦下单则不可撤销和不可退换。无断裂耳固定杆-EB-2BNarishige的 EB-2B是专门用于SN系列仪器的耳固定杆。类型B有专门设计的尖端，不会伤害柔软的耳孔。也请考虑以下这些耳固定杆：EB-2A 专用耳固定杆（中空型，一对）EB-2C 专用大鼠耳固定杆（一对）EB-2D 专用豚鼠耳固定杆（一对）规格长度/重量长140mm, 95g (per pair)*这是一个NARISHIGE“组2”的特殊订单项目。因为需要根据用户需求进行定制，订货交付时间通常为4周，一旦下单则不可撤销和不可退换。立体定位仪耳杆EB-1 大鼠耳固定杆-EB-2CNarishige的EB-2C是专门用于SN系列仪器的耳固定杆。类型C与适配器RA-4结合，用于大鼠。也请考虑以下这些耳固定杆：EB-2A 专用耳固定杆（中空型，一对）EB-2B 专用耳固定杆（无断裂型，一对）EB-2D 专用豚鼠耳固定杆（一对）规格长度/重量长140mm, 95g (per pair)*这是一个NARISHIGE“组2”的特殊订单项目。因为需要根据用户需求进行定制，订货交付时间通常为4周，一旦下单则不可撤销和不可退换。立体定位仪耳杆EB-1 豚鼠耳固定杆-EB-2DNarishige的EB-2D是专门用于SN系列仪器的耳固定杆。类型D与适配器RA-3相结合用于豚鼠。也请考虑下面这些耳固定杆：EB-2A 专用耳固定杆（中空型，一对）EB-2B 专用耳固定杆（无断裂型，一对）EB-2C 专用大鼠耳固定杆（一对）规格长度/重量长140mm, 95g (per pair)*这是一个NARISHIGE“组2”的特殊订单项目。因为需要根据用户需求进行定制，订货交付时间通常为4周，一旦下单则不可撤销和不可退换。用于SR系列的大鼠耳固定杆-EB-3ANarishige的 EB-3A是用于SR系列立体定位仪器的大鼠耳固定杆。规格尺寸/重量长70mm, 36g用于SR系列的小鼠耳固定杆-EB-3BNarishige的 EB-3B 是用于SR系列立体定位仪器的小鼠耳固定杆。规格尺寸/重量长70mm, 36g大鼠耳固定杆辅助器-EB-4NNarishige的EB-4N 是大鼠耳固定杆的辅助器，用于SR系列立体定位仪器。在将大鼠放置在立体定位仪器之前，可把辅助器固定到大鼠。用户通过平缓的滑移系统可以感受到细腻的触感。使用后，EB-4N可以进行拆卸清洗。规格尺寸/重量宽36-53 x 深37 x 10mm, 32g小鼠耳固定杆辅助器-EB-5NNarishige 的 EB-5N是小鼠耳固定杆的辅助器，用于SR系列立体定位仪器。在将小鼠放置在立体定位仪器之前，可把辅助器固定到小鼠。用户通过平缓的滑移系统可以感受到细腻的触感。使用后，EB-5N可以进行拆卸清洗。尺寸/重量宽36-53 x 深37 x 10mm, 32g