HAT培养基添加剂 (50×)

HAT培养基添加剂 (50×)

HAT Supplement (50×)

产品规格：10ml ‍

保存条件: Store at -20℃.

产品介绍:

   HAT Supplement is a liquid mixture of sodium hypoxanthine, aminopterin and thymidine. After dilution, HAT is suitable for use as a post-fusion selective medium to eliminate unfused or self-fused HGPRT-myeloma cells. 

  γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma; 

Application:

HAT Media Supplement (50×) Hybri-Max has been used:

in the selection and validation of hypoxanthine-guanine phosophoribosyltransferase (HPRT)-deficient clones.

in the generation of mAbs (monoclonal antibodies).

in the luciferase-based bioassay (LUC) assay.

Reconstitution:

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！