hCMEC/D3永生化人脑微血管内皮细胞株

hCMEC/D3永生化人脑微血管内皮细胞株

英文名称：HCMEC/D3; CMEC/D3

描述：The hCMEC/D3 celll line was derived from human temporal lobe microvessels isolatedfrom tissue excised during surgery for control of epilepsy. The primary isolate wasenriched in cerebral endothelial cells (CECs). In the first passage, cells weresequentially immortalized by lentiviral vector transduction with the catalytic subunit ofhuman telomerase (hTERT) and SV40 large T antigen, following which CEC wereselectively isolated by limited dilution cloning, and clones were extensivelycharacterized for brain endothelial phenotype. This brain microvascular endothelialcell line represents one such model of the human BBB that can be easily grown and isamenable to cellular and molecular studies on pathological and drug transport

mechanisms with relevance to the central nervous system (CNS).

种属：人

组织来源：脑微血管

形态：内皮样

生长方式：贴壁

无菌检测：细菌、酵母、支原体检测匀为阴性

病原体检查：HIV、乙型肝炎、丙型肝炎检查均为阴性

运输方式：复苏发货或干冰冷冻发货

安全性：所有肿瘤和病毒转染的细胞均视为有潜在的生物危害性,必须在二级生物安全台内操作,并请注意防护。

温度: 37°C

气相: 95%空气, 5%二氧化碳

保存：冻存条件:细胞保存液

保存条件:液氮存储

供应限制：仅供科学研究之用。