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产品介绍
超敏ECL发光液
| 产品编号 | 产品名称 | 规格 | 价格(元) | 说明书 |
| ZY-62301 | 超敏ECL化学发光液 | 50ml/500ml | 300/2,000 |
| Western Blot结果的好坏与否与ECL发光液的选择有着密切关系，好的ECL发光液不仅可曝光得到漂亮的目的条带，还可节省昂贵的抗体。海基超敏ECL发光液用于Western、Northern和Southern blot分子杂交的化学发光检测，原理是采用新型发光底物（Luminol），其与辣根过氧化物酶反应时产生很强的发光信号，以此检测微量蛋白而获得理想的实验结果。比传统化学显色法灵敏度高，可达pg水平，发光持续时间长，信号30min内不会发生明显降低。 |  | |
组分 | ||
| 组分名称数量保存Super ECL A25 ml4℃Super ECL B25 ml4℃ | ||
应用 | ||
| 在Western Blot印迹分析，核酸杂交以及EMSA实验中，HRP标记的二抗或探针与靶分子结合再与底物反应产生可见光。 | ||
储存 | ||
| 2-8°C，Super ECL A要求避光保存。 | ||
实例 | ||
 |  | |
| Fig1.检测Hela细胞裂解物的GAPDH蛋白，应用超敏ECL发光液(,M2301)显色，一抗为兔抗GAPDH多克隆抗体，稀释比例为1:1000。 | Fig2.检测A549细胞裂解物的AKT蛋白，应用超敏ECL发光液(和Competitor P公司的ECL发光液分别显色， 一抗为AKT rabbit mab(CST,#4691)，稀释比例为1:1000。 | |
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应用本产品文献 | ||
| 1.Calcium oxalate crystals induces tight junction disruption in distal renal tubular epithelial cells by activating ROS/Akt/p38 MAPK signaling pathway. 2.Nrf2 mediates the protective effects of homocysteine by increasing the levels of GSH content in HepG2 cells. 3.EHD1 confers resistance to cisplatin in non-small cell lung cancer by regulating intracellular cisplatin concentrations. 4.Nuclear factor erythroid 2-related factor 2 activation mediates hyperhomocysteinemia-associated lipolysis suppression in adipocytes. 5.Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L. 6.Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content. 7.The Wnt/β-Catenin/LEF1 Pathway Promotes Cell Proliferation at Least in Part Through Direct Upregulation of miR-17-92 Cluster. 8.Membrane-permeabilized sonodynamic therapy enhances drug delivery into macrophages. 9.MicroRNA‐125a inhibits tumorigenesis by targeting Smurf1 in colorectal carcinoma. 10.Double Knockdown of PHD1 and Keap1 Attenuated Hypoxia-Induced Injuries in Hepatocytes. 11.Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells. 12.LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non‐small cell lung cancer. 13.Wnt4 is significantly upregulated during the early phases of cisplatin-induced acute kidney injury. 14.CCAT2 is an oncogenic long non-coding RNA in pancreatic ductal adenocarcinoma. 15.Chronic Moderate Alcohol Intakes Accelerate SR-B1 Mediated Reverse Cholesterol Transport. 16. Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway. 17.Tissue cholesterol content alterations in streptozotocin-induced diabetic rats. 18.Arsenic trioxide promotes senescence and regulates the balance of adipogenic and osteogenic differentiation in human mesenchymal stem cells. 19.STAT1 negatively regulates hepatocellular carcinoma cell proliferation. 20.Preparation and Characterization of a Novel Aspirin Derivative with Anti-Thrombotic and Gastric Mucosal Protection Properties. 21.Effects of hepatocyte growth factor onMMP-2 expression in scleral fibroblasts from a guinea pig myopia model. 22.Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells. | ||
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超敏ECL发光液信息由上海泽叶生物科技有限公司为您提供，如您想了解更多关于超敏ECL发光液报价、型号、参数等信息，欢迎来电或留言咨询。除供应超敏ECL发光液外，上海泽叶生物科技有限公司还可为您提供OPRM1试剂盒；人黏液型阿片样受体检测试剂盒（ELISA方法）、SIGLEC2；人唾液酸结合Ig样凝集素2检测试剂盒、SIGLEC8；人唾液酸结合Ig样凝集素8检测试剂盒等产品，公司有专业的客户服务团队，是您值得信赖的合作伙伴。
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2016-08-09
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