Anti-APOER2 antibody

Anti-APOER2 antibody

• 种属反应性Human

• 验证应用WB,ICC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein corresponding to N terminal of Human APOER2.

• 偶联Non-conjugated

• Anti-APOER2 antibody性能

• 形态Liquid

• 浓度2 mg/ml.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG2a

• 纯化方式Protein A purified

• 亚细胞定位Cell membrane, Membrane, Secreted.

  
  

• 其它名称

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• APOER2 antibody

• Apolipoprotein E receptor 2 antibody

• low density lipoprotein receptor-related protein 8 antibody

• Anti-APOER2 antibody应用

  WB: 1:500
  ICC: 1:50

• 

  Fig1: Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: A549 cell lysate
  Lane 2: SHSY5Y cell lysate
  Lane 3: SKBR-3 cell lysate
  Lane 4: U937 cell lysate

  

  Fig2: ICC staining of APOER2 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

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