Anti-Myc-tag antibody
验证应用WB,ELISA
抗体类型小鼠单抗
免疫原Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin..
偶联Non-conjugated
Anti-Myc-tag antibody性能
形态Liquid
浓度2 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
其它名称
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Anti-Myc-tag antibody应用
WB: 1:1,000-1:5,000
ELISA: Use at an assay dependent concentration.
Fig1: Western blot analysis of Myc-tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: N-terminal Myc-tagged recombinant protein lysate
Lane 2: C-terminal Myc-tagged recombinant protein lysate
Fig2: Immobilized C-terminal Myc-tagged recombinant protein. at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃.
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