Anti-SARS-CoV-2 Nucleocapsid Protein antibody
种属反应性SARS-CoV-2
验证应用ELISA, WB
抗体类型小鼠单抗
免疫原Recombinant SARS-CoV-2 Nucleocapsid Protein.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4).
亚型IgG1
纯化方式Protein G affinity purified.
亚细胞定位Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus.
其它名称
SARS-CoV-2 Nucleocapsid Protein antibody
SARS-CoV-2 NP antibody
nucleocapsid protein antibody
ELISA: Use at an assay dependent concentration.
WB: 1:500-1:5,000
Fig1: The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.
Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml.
Fig2: Western blot analysis of with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and Inactivated SARS-CoV-2 lysate (2) at 20ng.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
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企业名称
上海泽叶生物科技有限公司
企业信息已认证
企业类型
信用代码
91310116MAJ8N934E
成立日期
2016-08-09
注册资本
100
经营范围
生物科技
上海泽叶生物科技有限公司
公司地址
上海市松江区国家经济开发区北松公路5629号聚科生物松江园区
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