Anti-Histone H3 (tri methyl K9) antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P
抗体类型小鼠单抗
免疫原Synthetic peptide within N terminal human Histone H3 including tri methyl K9 conjugated to keyhole limpet haemocyanin.
偶联Non-conjugated
Anti-Histone H3 (tri methyl K9) antibody性能
形态Liquid
浓度1 mg/ml
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein G purified.
亚细胞定位Nucleus.
其它名称
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Anti-Histone H3 (tri methyl K9) antibody应用
WB:1:5,000-1:10,000
ICC:1:500-1:1,000
IHC-P:1:200
Fig1: Western blot analysis of Tri-Methyl-Histone H3 (Lys9) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane A: F9 cell lysate
Lane B: F9+non-methyl peptide
Lane C: F9+Tri-methl (lys9) peptide
Lane D: PC12 cell lysate
Lane E: NCCIT cell lysate
Fig2: ICC staining of Tri-Methyl-Histone H3 (Lys9) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution.
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Tri-Methyl-Histone H3 (Lys9) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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