Anti-Cyclin A2 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P
抗体类型小鼠单抗
免疫原recombinant protein
偶联Non-conjugated
Anti-Cyclin A2 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位--
Anti-Cyclin A2 antibody应用
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Fig1: Western blot analysis on different cell lysates using anti-Cyclin A2 Mouse mAb.
Positive control:
Lane 1: 293
Lane 2: F9
Lane 3: PC12
Lane 4: Hela
Fig2: ICC staining Cyclin A2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Cyclin A2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Hela cells with Cyclin A2 antibody at 1/100 dilution (black) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the second
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