Anti-CD31/PECAM-1 antibody
种属反应性Human,Mouse
验证应用WB,ICC/IF,IHC-P,FC
抗体类型小鼠单抗
免疫原Synthetic peptide (KLH-coupled) within C-terminal residues of human CD31.
偶联Non-conjugated
Anti-CD31/PECAM-1 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2a
纯化方式Peptide affinity purified
亚细胞定位Cell junction. Cell membrane. Membrane.
Anti-CD31/PECAM-1 antibody应用
WB: 1:1,000-1:5,000
ICC/IF: 1:50-1:200
IHC-P: 1:2,000-1:5,000
FC:1:50-1:100
Fig1:
Western blot analysis of CD31 on THP-1 cells lysates using anti-CD31 antibody at 1/1,000 dilution.
Fig2: ICC staining CD31 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD31 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse uterus muscle tissue using anti-CD31 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Jurkat cells with CD31 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary
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