Anti-HSPA5 antibody
种属反应性Human
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Peptides
偶联Non-conjugated
Anti-HSPA5 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -391℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein A purified.
亚细胞定位Endoplasmic reticulum lumen. Melanosome. Cytoplasm.
其它名称
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Anti-HSPA5 antibody应用
WB: 1:1000-1:5,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of HSPA5 on different cells lysates using anti-HSPA5 antibody at 1/1,000 dilution.
Positive control:
Line1: Hela
Line2: HepG2
Line3: NIH/3T3
Fig2: ICC staining HSPA5 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining HSPA5 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining HSPA5 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HSPA5 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of Hela cells with HSPA5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary
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