WB: 1:100-1:1,000 IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) IF: 1:50-1:500 IHC-P: 1:50-1:500
Fig1: Western blot analysis of Nrf1 expression in non-transfected Hela (A), TF-1 (B), K562 (C), NIH/3T3 (D), RPE-J (E) and 3T3-L1 (F) whole cell lysates.
Fig2: Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-Nrf1 antibody. Counter stained with hematoxylin.