Anti-NQO1 antibody

Anti-NQO1 antibody

• 种属反应性Human,Rat

• 验证应用WB,IHC-P

• 抗体类型小鼠单抗

• 免疫原Recombinant protein within Human NQO1 aa 1-285.

• 偶联Non-conjugated

• Anti-NQO1 antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG2b

• 纯化方式Protein affinity purified.

• 亚细胞定位Cytoplasm.

• 其它名称

  more

• Azoreductase antibody

• Cytochrome b 5 reductase antibody

• DHQU antibody

• Anti-NQO1 antibody应用

  WB:1:1,000-1:5,000
  IHC-P:1:50-1:100

• 

  Fig1: Western blot analysis of NQO1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: SiHa cell lysate, untreated
  Lane 2: A549 cell lysate, untreated

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody  at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NQO1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.

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