Anti-RUBCN antibody
种属反应性Human
验证应用WB
抗体类型小鼠单抗
免疫原Purified recombinant fragment of human RUBCN (AA: 859-972) expressed in E. Coli.
偶联Non-conjugated
Anti-RUBCN antibody性能
形态Liquid
浓度1 mg/mL
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS with 0.05% sodium azide.
亚型IgG1
纯化方式Protein G purified.
亚细胞定位Lysosome, late endosome, early endosome
Anti-RUBCN antibody应用
WB: 1:500-1:2,000
Fig1: Western blot analysis of RUBCN against human RUBCN (AA: 859-972) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of RUBCN against HEK293 (1) and RUBCN (AA: 859-972)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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