Anti-RALA antibody

Anti-RALA antibody

• 种属反应性Human,Mouse,Monkey

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• Anti-RALA antibody性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cell surface. Cell membrane. Cleavage furrow.

• 其它名称

  more

• MGC48949 antibody

• Ral a antibody

• Ral A protein antibody

• Anti-RALA antibody应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of RALA on human RALA recombinant protein using anti-RALA antibody at 1/1,000 dilution.

  

  Fig2: Western blot analysis of RALA on HEK293 (1) and RALA-hIgGFc transfected HEK293 (2) cell lysate using anti-RALA antibody at 1/1,000 dilution.

  

  Fig3: Western blot analysis of RALA on different cell lysate using anti-RALA antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HepG2
  Lane 2: MCF-7
  Lane 3: A549
  Lane 4: K562
  Lane 5: Raji
  Lane 6: MOLT4
  Lane 7: Hela
  Lane 8: COS7
  Lane 9: NIH/3T3

  

  Fig4: ICC staining RALA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-RALA antibody. Counter stained with hematoxylin.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RALA antibody. Counter stained with hematoxylin.

  

  Fig7: Flow cytometric analysis of MCF-7 cells with RALA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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