Fig3: Western blot analysis of CD299 against L-02 (1), HepG2 (2), BEL-7402 (3), SMMC-7702 (4), and HL-7702 (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.