Anti-NBN antibody
种属反应性Human,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-NBN antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2a
纯化方式Protein A purified.
亚细胞定位Nucleus. Nucleus, PML body.
其它名称
more
AT V1 antibody
AT V2 antibody
ATV antibody
Anti-NBN antibody应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of p95 NBS1 on human p95 NBS1 recombinant protein using anti- p95 NBS1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of p95 NBS1 on HEK293 (1) and p95 NBS1 -hIgGFc transfected HEK293 (2) cell lysate using anti- p95 NBS1 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of p95 NBS1 on different cell lysate using anti- p95 NBS1 antibody at 1/1,000 dilution.
Positive control: Line1: A549 Line2: Jurkat Line3: PC-12
Fig4: ICC staining p95 NBS1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti- p95 NBS1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- p95 NBS1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with p95 NBS1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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