Anti-GFPT1 antibody
种属反应性Human,Monkey,Rat
验证应用WB,ICC,FC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-GFPT1 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Cytosol. Extracellular exosome.
其它名称
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Anti-GFPT1 antibody应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:100-1:200
Fig1: Western blot analysis of GFPT1 on human GFPT1 recombinant protein using anti-GFPT1 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of GFPT1 on HEK293 (1) and GFPT1-hIgGFc transfected HEK293 (2) cell lysate using anti-GFPT1 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of GFPT1 on different cell lysate using anti-GFPT1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: HEK293
Lane 3: BEL-7402
Lane 4: SMMC-7721
Lane 5: SK-MES-1
Lane 6: C6
Lane 7: COS7
Fig4: ICC staining GFPT1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with GFPT1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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