Anti-CD49D antibody

Anti-CD49D antibody

• 种属反应性Human

• 验证应用WB,ICC,FC

• 抗体类型小鼠单抗

• 免疫原Purified recombinant fragment of human CD49D (AA: extra 833-977) expressed in E. Coli.

• 偶联Non-conjugated

• Anti-CD49D antibody性能

• 形态Liquid

• 浓度1 mg/mL

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS with 0.05% sodium azide.

• 亚型IgG2a

• 纯化方式Protein G purified.

• 亚细胞定位Membrane.

• 其它名称

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• 269C wild type antibody

• Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody

• CD49 antigen like family member D antibody

• Anti-CD49D antibody应用

  WB: 1:500-1:2,000
  ICC: 1：50-1:200
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of CD49D against human CD49D (AA: extra 833-977)) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of  against HEK293 (1) and CD49D (AA: extra 833-977))-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig3: Western blot analysis of 0 against HL-60 (1) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig4: Flow cytometric analysis of CD49D was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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