Fig3: Western blot analysis of CD114 against A431 (1), K562 (2), THP-1 (3), MOLT4 (4), and Jurkat (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibodywas used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.